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 UNITED
STATES

 SECURITIES
AND EXCHANGE COMMISSION

 Washington,
D. C. 20549

 FORM

 Annual
 Report Pursuant to Section 13 or 15( d)
 of the Securities Exchange Act of 1934

 For
 the fiscal year ended December 31, 2024 or

 TRANSITION
 REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934

 For
 the transition period from __________ to __________

 Commission
file number: 001-34810

 (Exact
name of registrant as specified in its charter)

 Delaware

 33-0595156

 (State
 or Other Jurisdiction of Incorporation or Organization)

 (I.R.S.
 Employer Identification No.)

 12117
 Bee Caves Road, Building III, Suite 100

 Austin,
 Texas

 78738

 (Address
 of Principal Executive Offices)

 (Zip
 Code)

 Registrant's
telephone number, including area code: (512) 519-0400

 Securities
registered pursuant to Section 12(b) of the Act:

 Title
 of each class

 Trading
 Symbol(s)

 Name
 of each exchange on which registered

 Common
 Stock, par value $0.001 per share

 AWH

 The
 Nasdaq Capital Market

 Securities
registered pursuant to Section 12(g) of the Act: None

by check mark if the registrant is a well-known seasoned issuer, as defined in Rule 405 of the Securities Act. Yes No

by check mark if the registrant is not required to file reports pursuant to Section 13 or Section 15(d) of the Act. Yes No

by check mark whether the registrant (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange
Act of 1934 during the preceding 12 months (or for such shorter period that the registrant was required to file such reports), and (2)
has been subject to such filing requirements for the past 90 days. Yes No

by check mark whether the registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule
405 of Regulation S-T (§ 232.405 of this chapter) during the preceding 12 months (or for such shorter period that the registrant
was required to submit such files). Yes No

by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting
company, or an emerging growth company. See the definitions of "large accelerated filer," "accelerated filer,"
"smaller reporting company" and "emerging growth company" in Rule 12b-2 of the Exchange Act.

 Large
 accelerated filer

 Accelerated
 filer

 Non-accelerated
 filer

 Smaller
 reporting company

 Emerging
 growth company

 If
an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying
with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act.

by check mark whether the registrant has filed a report on and attestation to its management's assessment of the effectiveness
of its internal control over financial reporting under Section 404(b) of the Sarbanes-Oxley Act (15 U.S.C. 7262(b)) by the registered
public accounting firm that prepared or issued its audit report. Yes No

 If
securities are registered pursuant to Section 12(b) of the Act, indicate by check mark whether the financial statements of the registrant
included in the filing reflect the correction of an error to previously issued financial statements.

by check mark whether any of those error corrections are restatements that required a recovery analysis of incentive-based compensation
received by any of the registrant's executive officers during the relevant recovery period pursuant to §240.10D-1(b).

by check mark whether the registrant is a shell company (as defined in Rule 12b-2 of the Act). Yes No

 The
aggregate market value of voting common stock held by non-affiliates of the registrant is $ 21,088,293 and is based upon the last sales
price as quoted on The Nasdaq Capital Market as of June 30, 2024.

 As
of March 25, 2025, the registrant had 29,764,248 shares of common stock, par value $ 0.001 per share, outstanding.

 DOCUMENTS
INCORPORATED BY REFERENCE

information from the registrant's Definitive Proxy Statement for its 2025 Annual Meeting of Stockholders is incorporated by reference
into Part III of this report.

 ASPIRA
WOMEN'S HEALTH INC.

 Table
of Contents

 Page
 No.

 ITEM
 1.

 Business

 ITEM
 1A.

 Risk Factors

 ITEM
 1B.

 Unresolved Staff Comments

 ITEM
 1C.

 Cybersecurity

 ITEM
 2.

 Properties

 ITEM
 3.

 Legal Proceedings

 ITEM
 4.

 Mine Safety Disclosures

 ITEM
 5.

 Market For Registrant's Common Equity, Related Stockholder Matters and Issuer Purchases of Equity Securities

 ITEM
 6.

 [Reserved]

 ITEM
 7.

 Management's Discussion and Analysis of Financial Condition and Results of Operations

 ITEM
 7A.

 Quantitative and Qualitative Disclosures About Market Risk

 ITEM
 8.

 Consolidated Financial Statements and Supplementary Data

 ITEM
 9.

 Changes in and Disagreements With Accountants on Accounting and Financial Disclosure

 ITEM
 9A.

 Controls and Procedures

 ITEM
 9B.

 Other Information

 ITEM
 9C.

 Disclosure Regarding Foreign Jurisdictions that Prevent Inspections

 ITEM
 10.

 Directors, Executive Officers and Corporate Governance

 ITEM
 11.

 Executive Compensation

 ITEM
 12.

 Security Ownership of Certain Beneficial Owners and Management and Related Stockholder Matters

 ITEM
 13.

 Certain Relationships and Related Transactions, and Director Independence

 ITEM
 14.

 Principal Accountant Fees and Services

 ITEM
 15.

 Exhibits and Financial Statement Schedules

 ITEM
 16.

 Form 10-K Summary

 SIGNATURES

 The
following are registered and unregistered trademarks and service marks of Aspira Women's Health Inc.: VERMILLION SM,
 Aspira Women's Health, OVA1, OVERA,
 ASPiRA LABS, OvaCalc,
OVASUITE SM, ASPiRA GenetiX SM, OVA1PLUS, OVAWATCH,
 EndoCheck SM, ENDOINFORM TM, OVAINFORM TM, OvaInherit SM,
 Aspira Synergy,, OVA360 SM, ASPIRA
IVD SM, and YOUR HEALTH, OUR
PASSION.

 i

 SPECIAL
NOTE REGARDING FORWARD-LOOKING STATEMENTS

 This
Annual Report on Form 10-K contains forward-looking statements, within the meaning of Section 27A of the Securities Act of 1933, as amended
(the "Securities Act"), and Section 21E of the Securities Exchange Act of 1934, as amended (the "Exchange Act"),
about us and our industry that involve substantial risks and uncertainties.

 These
statements involve a number of risks and uncertainties. Words such as "may," "expects," "intends,"
"anticipates," "believes," "estimates," "plans," "seeks," "could,"
"should," "continue," "will," "potential," "projects" and similar expressions
are intended to identify such forward-looking statements. Readers are cautioned that these forward-looking statements speak only as of
the date on which the was filed with the Securities and Exchange Commission (the "SEC"), and, except as required by law,
Aspira Women's Health Inc. ("Aspira" and, together with its subsidiaries, the "Company," "we,"
"our," or "us") does not assume any obligation to update, amend or clarify them to reflect events, new information
or circumstances occurring after such date.

 Examples
of forward-looking statements include, without limitation:

 projections
 or expectations regarding our future test volumes, revenue, average unit price, cost of revenue, operating expenses, research and
 development expenses, gross profit margin, cash flow, results of operations and financial condition;

 the
 ability to maintain the listing of our common stock and public warrants on The Nasdaq Capital Market;

 our
 plan to broaden our commercial focus from ovarian cancer to differential diagnosis of women with a range of gynecological diseases,
 including additional pelvic disease conditions such as endometriosis and benign pelvic mass monitoring;

 our
 planned business strategy and the anticipated effects thereof, including partnerships such as those based on our Aspira Synergy platform,
 specimen or research collaborations, licensing arrangements, commercial collaborations and distribution agreements;

 plans
 to expand our current or future products to markets outside of the United States through distribution collaborations or out-licensing;

 plans
 to develop new algorithms, molecular diagnostic tests, products and tools and otherwise expand our product offerings;

 plans
 to develop, launch and establish payer coverage and secure contracts for current and new products, including ENDOinform (formerly
 EndoMDx) and OVAinform (formerly OvaMDx);

 regarding local and/or national coverage under Novitas, our Medicare Administrative Carrier;

 anticipated
 efficacy of our products, product development activities and product innovations, including our ability to improve sensitivity and
 specificity over traditional diagnostics;

 expected
 competition in the markets in which we operate;

 plans
 with respect to Aspira Labs, Inc. ("Aspira Labs"), including plans to expand Aspira Labs' testing capabilities,
 specifically molecular lab capabilities;

 regarding continuing future services provided by Quest Diagnostics Incorporated;

 regarding continuing future services provided by BioReference Health, LLC;

 plans
 to develop informatics products as laboratory developed tests ("LDTs") and potential Food and Drug Administration ("FDA")
 oversight changes of LDTs;

 regarding existing and future collaborations and partnerships for our products, including plans to enter into decentralized arrangements
 for our Aspira Synergy platform and to provide and expand access to our risk assessment tests;

 plans
 regarding future publications and presentations;

 regarding potential collaborations with governments, legislative bodies and advocacy groups to enhance awareness and drive policies
 to provide broader access to our tests;

 our
 ability to continue to comply with applicable governmental regulations, including regulations applicable to the operation of our
 clinical lab, expectations regarding pending regulatory submissions and plans to seek regulatory approvals for our tests within the
 United States and internationally, as applicable;

 our
 continued ability to expand and protect our intellectual property portfolio;

 anticipated
 liquidity and capital requirements;

 anticipated
 future losses and our ability to continue as a going concern;

 regarding raising capital and the amount of financing anticipated to be required to fund our planned operations;

 regarding attrition and recruitment of top talent;

 regarding the results of our clinical research studies and our ability to recruit patients to participate in such studies;

 our
 ability to use our net operating loss carryforwards and anticipated future tax liability under U.S. federal and state income tax
 legislation;

 expected
 market adoption of our current and prospective diagnostic tests, including Ova1, Overa, Ova1Plus, OvaWatch, ENDOinform and OVAinform,
 as well as our Aspira Synergy platform;

 regarding our ability to launch new products we develop, license, co-market or acquire;

 regarding the size of the markets for our products;

 regarding reimbursement for our products, and our ability to obtain such reimbursement, from third-party payers such as private insurance
 companies and government insurance plans;

 potential
 plans to pursue clearance designation with the FDA with respect to OvaWatch, ENDOinform and OVAinform;

 expected
 potential target launch timing for future products;

 regarding compliance with federal and state laws and regulations relating to billing arrangements conducted in coordination with
 laboratories;

 plans
 to advocate for legislation and professional society guidelines to broaden access to our products and services;

 ability
 to protect and safeguard against cybersecurity risks and breaches; and

 regarding the results of our academic research agreements.

 We
caution you that the foregoing list may not contain all of the forward-looking statements made in this Annual Report on Form 10-K.

 Other
sections of this Annual Report on Form 10-K may include additional factors that could harm our business and financial performance. Moreover,
we operate in a very competitive and rapidly changing environment. New risk factors emerge from time to time, and it is not possible
for our management to predict all risk factors, nor can we assess the impact of all factors on our business or the extent to which any
factor, or combination of factors, may cause actual results to differ materially from those contained in, or implied by, any forward-looking
statements.

 Forward-looking
statements are subject to significant risks and uncertainties, including those discussed in Part I Item 1A, "Risk Factors,"
that could cause actual results to differ materially from those projected in such forward-looking statements due to various factors,
including our ability to continue as a going concern; our ability to comply with the continued listing requirements of the Listing Qualifications
Department of The Nasdaq Stock Market, LLC ("Nasdaq"); impacts resulting from potential changes to coverage of Ova1 through
our Medicare Administrative Carrier for Ova1; anticipated use of capital and its effects; our ability to increase the volume of our product
sales; failures by third-party payers to reimburse for our products and services or changes to reimbursement rates; our ability to continue
developing existing technologies and to develop, protect and promote our proprietary technologies; plans to develop and perform LDTs;
our ability to comply with FDA regulations that relate to our products and to obtain any FDA clearance or approval required to develop
and commercialize medical devices; our ability to develop and commercialize additional diagnostic products and achieve market acceptance
with respect to these products; our ability to compete successfully; our ability to obtain any regulatory approval required for our future
diagnostic products; our or our suppliers' ability to comply with FDA requirements for production, marketing and post-market monitoring
of our products; our ability to maintain sufficient or acceptable supplies of immunoassay kits from our suppliers; in the event that
we succeed in commercializing our products outside the United States, the political, economic and other conditions affecting other countries;
changes in healthcare policy; our ability to comply with environmental laws; our ability to comply with the additional laws and regulations
that apply to us in connection with the operation of Aspira Labs; our ability to use our net operating loss carryforwards; our ability
to use intellectual property; our ability to successfully defend our proprietary technology against third parties; our ability to obtain
licenses in the event a third party successfully asserts proprietary rights; the liquidity and trading volume of our common stock; the
concentration of ownership of our common stock; our ability to retain key employees; our ability to secure additional capital on acceptable
terms to execute our business plan; business interruptions or force majeure or acts of God; the effectiveness and availability of our
information systems; our ability to integrate and achieve anticipated results from any acquisitions or strategic alliances; future litigation
against us, including infringement of intellectual property and product liability exposure; and additional costs that may be required
to make further improvements to our laboratory operations.

 SUMMARY
OF RISK FACTORS

 The
following is only a summary of the principal risks that may materially adversely affect our business, financial condition, results of
operations and cash flows. The following should be read in conjunction with the more complete discussion of the risk factors we fact,
which are set forth in the section entitled "Item 1A. Risk Factors" in this report.

 Risks
Related to Our Business and Industry

 If
 we are unable to increase the volume of OvaSuite sales, our business, results of operations and financial condition will be adversely
 affected.

 There
 is substantial doubt about our ability to continue as a going concern, and this may adversely affect our stock price and our ability
 to raise capital.

 We
 may need to sell additional shares of our common stock or other securities in the future to meet our capital requirements, which
 could cause significant dilution.

 We
 will need to raise additional capital in the future, and if we are unable to secure adequate funds on terms acceptable to us, we
 may be unable to execute our business plan.

 Failures
 by third-party payers to reimburse for our products and services or changes in reimbursement rates could materially and adversely
 affect our business, financial condition and results of operations. In addition, changes in medical society guidelines may also adversely
 affect payers and result in a material change in coverage, adversely affecting our business, financial condition and results of operations.

 to continue coverage of Ova1 through Novitas, our Medicare Administrative Contractor for Ova1, could materially and adversely affect
 our business, financial condition and results of operation.

 to expand commercial, Medicare or Medicaid coverage for our products could materially and adversely affect our business, financial
 condition and results of operations.

 We
 are currently offering and developing multiple tests as LDTs and intend to develop and perform LDTs at Aspira Labs in the future.
 FDA's newly-issued rule for LDTs, which will be phased in over a period of four years, will significantly change the regulatory
 landscape for LDTs. Unless the rule is overturned by a court or superseded by Congressional action, our currently marketed LDTs and
 those we develop in the future will be subject to new requirements including, for some tests, premarket authorization. The new rule
 will lead to additional compliance costs and may delay or prevent market entry for new or modified tests and there is a risk that
 their commercialization, and our results of operations and financial condition, will be negatively affected.

 Our
 diagnostic tests and software are subject to ongoing regulation by the FDA, and any delay by or failure of the FDA to authorize our
 diagnostic tests submitted to the FDA may adversely affect our business, results of operations and financial condition.

 If
 we or our suppliers fail to comply with FDA requirements for production, marketing and post-market monitoring of our products, we
 may not be able to market our products and services and may be subject to stringent penalties, product restrictions or recall.

 If
 our suppliers fail to produce acceptable or sufficient stock, fail to supply stock due to supply shortages, make changes to the design
 or labeling of their biomarker kits or discontinue production of existing biomarker kits or instrument platforms, we may be unable
 to meet market demand for OvaSuite products.

 The
 operation of Aspira Labs requires us to comply with numerous laws and regulations, which is expensive and time-consuming and could
 adversely affect our business, financial condition and results of operations, and any failure to comply could result in exposure
 to substantial penalties and other harm to our business.

 If we are unable to complete the required milestones under our federal award milestone-based funding agreement, our business, results
of operations and financial condition will be adversely affected.

 Our
 milestone-based funding from a federal award could be delayed or eliminated based on actions from the Trump Administration.

 Risks
Related to Intellectual Property and Product Liability

 If
 we fail to maintain our rights to utilize intellectual property directed to diagnostic biomarkers, we may not be able to offer diagnostic
 tests using those biomarkers.

 If
 a third party infringes on our proprietary rights, we may lose any competitive advantage we have as a result of diversion of our
 time, enforcement costs and the loss of the exclusivity of our proprietary rights.

 Operational
Risks

 Because
 our business is highly dependent on key executives and employees, our inability to recruit and retain these people could hinder our
 business plans.

 The
 operation of Aspira Labs and our Aspira Synergy business depends on the effectiveness and availability of our information systems,
 including the information systems we use to provide services to our customers and to store employee data. If our information technology
 systems or those third parties upon which we rely or our data, are or were compromised, we could experience adverse consequences
 resulting from such compromise, including but not limited to regulatory investigations or actions; litigation; fines and penalties;
 disruptions of our business operations; reputational harm; loss of revenue or profits; loss of customers or sales; and other adverse
 consequences.

 Risks
Related to Owning Our Stock

 If
 we fail to maintain compliance with the Nasdaq minimum listing requirements, our common stock will be subject to delisting. Our ability
 to publicly or privately sell equity securities and the liquidity of our common stock could be adversely affected if our common stock
 is delisted.

 Unless
 our common stock continues to be listed on a national securities exchange it will become subject to the so-called "penny stock"
 rules that impose restrictive sales practice requirements.

 PART
I

 ITEM
 1.

 BUSINESS

 Company
Overview

Women's Health Inc. is dedicated to the discovery, development, and commercialization of noninvasive, AI-powered tests to aid in
the diagnosis of gynecologic diseases, starting with ovarian cancer.

 We
plan to broaden our focus to the differential diagnosis of other gynecologic diseases that typically cannot be assessed through traditional
non-invasive clinical procedures. We expect to continue commercializing our existing and new technology and to distribute our tests through
our decentralized technology transfer service platform, Aspira Synergy. We also intend to continue to raise public awareness regarding
the higher sensitivity and negative predictive value for ovarian malignancy of Ova 1 as compared to cancer antigen 125 ("CA-125")
on its own for women with adnexal masses planned for surgery, as well as the performance of our machine learning algorithms in detecting
ovarian cancer risk in different racial and ethnic populations. We plan to continue to expand access to our tests among Medicaid patients
as part of our corporate mission to make the best care available to all women, and we plan to advocate for legislation and the adoption
of our technology in professional society guidelines to provide broad access to our products and services.

 We
expect our extensive experience with gynecologists and healthcare providers, along with the historical adoption of our OvaSuite tests,
to continue to drive growth as we introduce new products. We believe our ability to successfully develop novel AI-enabled assays is superior
to others based on our know-how and extensive experience in designing and successfully launching FDA-approved and laboratory developed
blood tests to aid in the diagnosis of ovarian cancer. Moreover, our history of successfully collaborating with world-class research
and academic institutions allows us to innovate and provide outstanding patient care.

 We
own and operate Aspira Labs, Inc., a research and commercial CLIA laboratory in Texas.

 Our
product pipeline is focused on two areas: ovarian cancer and endometriosis.

 In
ovarian cancer, we have developed clinical data to support the use of our OvaWatch test multiple times for the monitoring of an adnexal
mass. In the second quarter of 2024, we expanded the features of our commercially available OvaWatch test for monitoring of adnexal masses
through periodic testing at physician prescribed intervals, marking the successful completion of the vision for OvaSuite. The successful
expansion of the OvaWatch mass monitoring feature in the second quarter of 2024 resulted in a tenfold increase in the market for our
tests when compared to the addressable market for Ova1Plus of approximately 200,000 to 400,000 based on patients identified for surgery.
As a result, we believe the addressable market for our tests to have increased to between 2 and 4 million tests per year.

 Our
OVAinform development program continues to progress. OVAinform is a multi-marker test that combines serum proteins, clinical data (metadata),
and miRNA for assessing the risk of ovarian cancer in women with an adnexal mass. We believe that by including patients with genetic and familial risk, it will increase the addressable market to
2,800,000.

 In
endometriosis, we are developing and intend to introduce a new non-invasive test to aid in the diagnosis of this debilitating disease
that impacts millions of women worldwide. We completed the design of a protein-based non-invasive blood test to aid the detection of
endometrioma, one of the most common forms of endometriosis. The algorithm was confirmed with three independent cohorts and is an important
input for our ENDOinform program focused on developing a multi-marker test that combines serum proteins, clinical data (metadata) and
miRNA for the identification of endometriosis.

 Our
endometriosis portfolio addresses an even larger addressable market. According to the U.S. Department of Health and Human Services, endometriosis
affects more than 6.5 million women in the United States. We believe the proliferation of commercially available and in-development therapeutics
for the treatment of endometriosis will create a significant demand for a non-invasive diagnostic.

 Recent
developments:

 Our
current capital resources are not sufficient to fund our operations for the remainder of 2025. Accordingly, we have been pursuing strategic
alternatives and seeking to raise additional capital.

 On
March 5, 2025, we entered into a securities purchase agreement with certain existing accredited shareholders ("the "Purchasers")
for the issuance and sale in a private placement (the "March 2025 Private Placement") of an aggregate principal amount of
approximately $1,365,000 in the form of Senior Secured Convertible Promissory Notes (the "Convertible Notes").

 The
Convertible Notes, which are convertible into units consisting of one share of common stock and 2.25 warrants (the "March 2025
Warrants"), will be senior, secured obligations of the Company. Interest will accrue and be payable on a quarterly basis in
kind at 3.34%, the applicable federal rate at the time of the transaction. The Convertible Notes will mature on March 6, 2030, unless earlier
converted in accordance with the terms of the Convertible Notes. In addition, the Company shall have the option to convert the Convertible Notes into units at the Conversion Price if the sum
of the net proceeds from the sale of the Convertible Notes and the net proceeds from the sale of any shares of common stock and
warrants by the Company subsequent to the March 2025 Private Placement equals or exceeds $4 million.

 The
March 2025 Warrants are exercisable for five years at $0.25 per share for the first 24 months after issuance, and $0.50 per share thereafter.

 The holders of the Notes are entitled to three representatives on our board of directors.

 Scientific
Basis for Our Products:

 Science
of Biomarkers: Our focus on translational biomarkers and informatics enables us to address the market for novel diagnostic tests
that simultaneously measure multiple biomarkers. A biomarker is a biomolecule or variant biomolecule (e.g., DNA, RNA or protein) that
is present at measurably greater or lesser concentrations, or is present in an altered form, in a disease state versus a normal condition.
Conventional protein tests measure a single protein biomarker whereas most diseases are complex. We believe cancer and other complex
diseases are heterogeneous at the causative level (i.e., most diseases can be traced to multiple potential etiologies) and at the human
response level (i.e., each individual afflicted with a given disease can respond to that ailment in a specific manner). Protein biomarkers
(our entrenched technology), miRNA molecular biomarkers and metadata (age, body mass index, etc.) each provide independent and non-overlapping
evidence for a disease state. This increases the accuracy, sensitivity and specificity of the test in most cases.

 Consequently,
measuring a single biomarker when multiple biomarkers may be altered in a complex disease is unlikely to provide meaningful information
about the disease state.

 We
believe that our approach of monitoring and combining multiple biomarkers using a variety of analytical techniques has allowed and will
continue to allow us to create diagnostic tools that provide information about the disease state with sufficient sensitivity and specificity
to aid the physician considering treatment options for patients with complex diseases. Such assays are sometimes referred to as Multivariate
Index Assays ("MIAs") and often utilize advanced algorithms based on logistic regression, pattern recognition and the like.
Often, MIA algorithms are non-intuitive and; therefore, require rigorous clinical validation and error modeling. Aspira and its collaborators
are considered experts in these areas and, in the case of Ova1 and Overa, presented both the clinical validation and error modeling needed
to gain pre-market authorization from the FDA. In the case of Ova1, the FDA granted a request for de novo classification of an
ovarian adnexal mass assessment score test system, a type of in vitro diagnostic device; in the case of Overa (previously Ova1 Next Generation),
FDA granted a 510(k) clearance.

 Our
Business and Products:

 We
currently commercialize the following blood test products and related services:

 (1)
the Ova1Plus workflow, which uses Ova1 as the primary test and Overa as a reflex for Ova1 intermediate range results. Ova1 is a qualitative
serum test intended as an aid to further assess the likelihood of malignancy in women with an ovarian adnexal mass for which surgery
is planned when the physician's independent clinical and radiological evaluation does not indicate malignancy. Overa is a second-generation
biomarker test intended to maintain Ova1's high sensitivity while improving specificity. The Ova1Plus workflow leverages the strengths
of Ova1's MIA sensitivity and Overa's (MIA2G) specificity to increase performance; and

 (2)
OvaWatch, which is intended to assist in the initial and periodic clinical assessment of malignancy risk in all women thought to have
an indeterminate or benign adnexal mass.

 Our
products are distributed through our own national sales force, including field sales, inside sales and a contracted sales team,
through our proprietary decentralized testing platform and cloud service, marketed as Aspira Synergy, and through marketing and
distribution agreements with BioReference Health, LLC. ("BioReference") and ARUP Laboratories. In November of 2024, we
expanded our distribution agreement with BioReference to include OvaWatch. This timing aligns with our approval from New York State
to sell OvaWatch and increases our ability to market the test in New York. This important addition will allow providers who
currently order Ova1 through BioReference to also order Aspira's products for any woman with a mass within their existing
BioReference workflows.

 Our
Ova1 test received FDA de novo classification in September 2009. Ova1 comprises instruments, assays, reagents, and the OvaCalc
software, which includes a proprietary algorithm that produces a risk score. Our Overa test, which includes an updated version of OvaCalc,
received FDA 510(k) clearance in March 2016. Ova1, Overa and OvaWatch, our first LDT, each use the Roche Cobas 4000, 6000 and 8000 platforms
for analysis of proteins. Revenue from Ova1 and OvaWatch is included in the results of operations in total revenue for the year ended
December 31, 2024.

 In
2021, we began entering into decentralized arrangements with large healthcare networks and physician practices for our Aspira Synergy
platform, our decentralized testing platform and cloud service for decentralized global access of protein biomarker testing. Ova1, Overa,
and the Ova1Plus workflow continue to be available through the Aspira Synergy platform. As of December 31, 2024, we had two active Aspira
Synergy contracts.

 OvaWatch
has been developed and is validated for use in Aspira's CLIA-certified lab as a non-invasive blood-based risk assessment test for
use in conjunction with clinical assessment and imaging to determine ovarian cancer risk for patients with an adnexal mass whose adnexal
mass has been determined by an initial clinical assessment as indeterminate or benign. OvaWatch is the only commercially available blood
test available for assessment of the risk of ovarian cancer in women diagnosed with an adnexal mass considered indeterminate or benign
by a physician's preliminary clinical assessment.

 We
collected clinical data to support the utility of OvaWatch to aid in surgical referral and as a longitudinal monitoring test, resulting
in two manuscripts that were published in peer review journals in May 2024. In addition, an abstract highlighting data evaluating the
use of OvaWatch to assess ovarian cancer risk in pre- and post-menopausal women was accepted for a poster presentation at the Annual
Meeting of The Menopause Society in September 2024.

 Outside
of the United States, we sponsored studies in the Philippines aimed at validating Overa and Ova1 in specific populations. In February
2024, we signed an exclusive license agreement with Hi-Precision Laboratories to offer OvaSuite tests in the Philippines. In November
2024, Hi-Precision Laboratories communicated the completion of all laboratory and regulatory processes required for it to offer Ova1Plus
commercially to patients in the Philippines under the terms of our licensing agreement. Accordingly, it began marketing the test to physicians
through its existing sales and marketing channels at that time. We intend to assist Hi-Precision in the design and execution of its commercialization
and physician adoption strategy. Building on the successful launch of Ova1Plus in the Philippines, we have created a process roadmap
for future global expansion efforts.

 We
own and operate Aspira Labs, based in Austin, Texas, a Clinical Chemistry and Endocrinology Laboratory accredited by the College of American
Pathologists, which specializes in applying biomarker-based technologies to address critical needs in the management of gynecologic cancers
and disease. Aspira Labs provides expert diagnostic services using a state-of-the-art biomarker-based risk assessment to aid in clinical
decision making and advance personalized treatment plans. The lab currently performs our Ova1Plus workflow and OvaWatch testing, and
we plan to expand the testing to other gynecologic conditions with high unmet need. Aspira Labs holds a CLIA Certificate of Accreditation
and a state laboratory license in California, Maryland, New York, Pennsylvania and Rhode Island. The Centers for Medicare & Medicaid
Services ("CMS") issued a supplier number to Aspira Labs in 2015. Aspira labs also hold a current ISO 13485 certification
which is the most accepted standard worldwide for medical device.

 In
the United States, revenue for diagnostic tests comes from several sources, including third-party payers such as insurance companies,
government healthcare programs, such as Medicare and Medicaid, client bill accounts and patients. Novitas Solutions, a Medicare Administrative
Carrier, covers and reimburses for Ova1 tests performed in certain states, including Texas. Due to our billed Ova1 tests being performed
exclusively at Aspira Labs in Texas, the Local Coverage Determination ("LCD") from Novitas Solutions provides national coverage
for patients enrolled in Medicare as well as Medicare Advantage health plans. We have applied for an LCD for OvaWatch, which remains
under review.

 In
November 2016, the American College of Obstetricians and Gynecologists ("ACOG") issued Practice Bulletin Number 174 which
included Ova1, defined as the "Multivariate Index Assay", outlining ACOG's clinical management guidelines for adnexal
mass management. Practice Bulletin Number 174 recommends that obstetricians and gynecologists evaluating women with adnexal masses who
do not meet Level A criteria of a low-risk transvaginal ultrasound should proceed with Level B clinical guidelines. Level B guidelines
state that the physician may use risk assessment tools such as existing CA-125 technology or Ova1 ("Multivariate Index Assay")
as listed in the bulletin. Based on this, Ova1 achieved parity with CA-125 as an ACOG Level B clinical recommendation for the management
of adnexal masses.

 Practice
Bulletins summarize current information on techniques and clinical management issues for the practice of obstetrics and gynecology. Practice
Bulletins are evidence-based documents, and recommendations are based on the evidence. This is also the only clinical management tool
used for adnexal masses. Although there are Practice Bulletins, guidelines do not exist for adnexal masses. ACOG guidelines do exist,
however, for ovarian cancer management.

Pipeline

 We
aim to introduce new gynecologic diagnostic products and to expand our product offerings to additional women's gynecologic health
diseases by adding additional gynecologic bio-analytic solutions involving biomarkers, clinical risk factors and patient data to aid
diagnosis and risk stratification. Future product expansions will be accelerated by the development of lab developed testing in a CLIA
environment, relationships with strategic research and development partners, and access to specimens in our biobank.

 OVAinform
 is a multi-marker test that combines serum proteins, clinical data (metadata), and miRNA for assessing the risk of ovarian cancer
 in women with an adnexal mass. The test is being developed in collaboration with Harvard's Dana-Farber Cancer Institute (providing
 clinical and trial design expertise), Brigham & Women's Hospital (providing miRNA technical expertise), and Medical University
 of Lodz (providing miRNA biomarker and bioinformatics analytic support).

 The
 miRNAs used in the OVAinform test were the subject of a 2017 paper, " Diagnostic potential for a serum miRNA neural network
 for detection of ovarian cancer " published in the peer-reviewed journal Cancer Biology. In October 2023, a poster entitled
 " Improving the diagnostic accuracy of an ovarian cancer triage test using a joint miRNA-protein model," was presented
 at the AACR Special Conference in Cancer Research: Ovarian Cancer by senior author, Dr. Kevin Elias M.D., Director, Gynecologic Oncology
 Laboratory at Brigham and Women's Hospital and Assistant Professor of Obstetrics, Gynecology and Reproductive Biology at Harvard
 Medical School. The poster highlighted data from a study that combined serum protein and patient clinical information (metadata)
 from Aspira's ovarian cancer registry studies with miRNA determined by the Elias laboratory. The data showed that using miRNA
 in combination with serum proteins, provided superior performance over existing ovarian cancer risk assessment blood tests.

 We
 have tested our entire set of selected miRNA biomarkers and, based on their performance, we are refining the features on our droplet
 digital PCR commercial platform. As a next step, we intend to increase our patient sample testing to refine the algorithm.

 ENDOinform
 (formerly EndoMDx) is a multi-marker test program that combines serum proteins, clinical data (metadata), and miRNA for the
 identification of endometriosis. The test is being developed in collaboration with a consortium of academic and clinical partners
 led by Dana-Farber Cancer Institute. We are currently in the process of analyzing the first 180 patient samples to verify protein
 and miRNA biomarkers. These investigations will establish analytical properties on our droplet digital PCR commercial platform for
 miRNA detection. Additionally, this data set will provide information on initial disease classification capability of miRNA and
 proteins. This is a critical step in evaluating the strength of algorithms that incorporate miRNAs and proteins.

 Studies
and Publications

 On
May 7, 2024, we announced the publication of two peer-reviewed manuscripts. The first manuscript, entitled " Ovarian Cancer Surgical
Consideration is Markedly Improved by the Neural Network Powered-MIA3G Multivariate Index Assay " was published in the peer-reviewed
journal Frontiers of Medicine on May 2, 2024. The findings of this study demonstrate that use of OvaWatch to stratify risk in patients
with an adnexal mass might help to reduce surgical backlogs and unnecessary surgical referrals. The second manuscript, entitled " Neural
Network-derived Multivariate Index Assay Demonstrates Effective Clinical Performance in Longitudinal Monitoring of Ovarian Cancer Risk "
was published in the journal Gynecologic Oncology on May 3, 2024. The findings of this study demonstrate that OvaWatch could be an effective
tool for the monitoring of ovarian cancer risk over time in women with indeterminate or low risk adnexal masses. Based on common practice
for adnexal mass management and consistent with the study, OvaWatch can be drawn by the provider every three to six months for active
surveillance of an adnexal mass.

 A
publication entitled Serum miRNA improves the accuracy of a multivariate index assay for triage of an adnexal mass was published
in the journal Gynecologic Oncology, August of 2024 from the laboratory of our collaborator Dr. Kevin Elias at the Brigham and
Women's Hospital. The paper describes the novel combination of microRNAs (miRNAs) and serum proteins to achieve increased performance
in the assessment of malignancy risk in patients with an adnexal mass. The miRNAs, discovered by Dr Elias' team, in combination
with serum proteins from Aspira Women's Health's proprietary multivariate index assays Ova1 and Overa showed increased sensitivity
for detection of malignancy and broader detection of diverse ovarian cancer subtypes. This publication establishes the feasibility of
improved tests using multi-omic information. ( Webber JW, Wollborn L, Mishra S, Vitonis AF, Cramer DW, Phan RT, Pappas TC, Stawiski
K, Fendler W, Chowdhury D, Elias KM. Serum miRNA improves the accuracy of a multivariate index assay for triage of an adnexal mass. Gynecol
Oncol. 2024 Aug 23;190:124-130.)

 An
abstract entitled " Application of a Deep Neural Network-Based Algorithm to Provide Additional Information in the Assessment
of Adnexal Masses Classified as Indeterminate by Imaging " was presented as a poster at the Annual Meeting of The Menopause
Society in September 2024. This presentation highlighted data evaluating the use of OvaWatch to assess ovarian cancer risk in pre- and
post-menopausal women. The data demonstrated that in women with an adnexal mass and an indeterminate ultrasound imaging result, the OvaWatch
result indicated low malignant potential of the mass in more than 70% of patients. The use of OvaWatch could provide additional information
to reduce surgical referrals.

 An
EndoCheck-related abstract entitled " Association of the Endometriosis Health Profile-5 (EHP-5) with Non-Invasive Biomarkers
in Patients with Suspected Endometriosis " was presented as a poster at the 27 th Annual National Association of Nurse
Practitioner's in Women's Health Women's Healthcare Conference in September 2024. This poster examined the association
of biomarkers for ovarian endometriosis (endometrioma) with quality-of-life survey responses before and after surgical intervention.
There was no association between endometrioma biomarkers and self-reported patient quality of life either prior to or after surgery,
and this was consistent with other research.

 An
EndoCheck-related virtual poster entitled " A Proprietary Protein-Based Algorithm May Increase Sensitivity of Endometrioma Detection
When Combined with Imaging " was presented at the annual meeting of the American Association of Gynecologic Laparoscopists in
November 2024. This poster summarizes a preliminary study on the performance of imaging combined with a protein biomarker-based algorithm.
The combination of these diagnostic tools resulted in increased sensitivity of detection of endometrioma and could be effective in risk
assessment and surgical planning for this condition.

 The
Diagnostic Field

 The
economics of healthcare demand effective and efficient allocation of resources which can be accomplished through disease prevention,
early detection of disease leading to early intervention, and diagnostic tools that can triage patients to more appropriate therapy and
intervention. In 2024, Fortune Business Insight, a market research and business consulting partnership, published a study which forecasts
the global in vitro diagnostic ("IVD") market to reach $117.6 billion by 2032, growing at a compound annual growth
rate of 6.0% from 2024 to 2032. We have chosen to concentrate our business focus in the areas of gynecologic oncology and disease where
we have established strong key opinion leaders, and provider and patient relationships. Demographic trends suggest that, as the population
ages, the burden from gynecologic diseases will increase and the demand for quality diagnostic, prognostic and predictive tests will
escalate. In addition, the areas of gynecologic oncology and disease generally lack quality diagnostic tests and, therefore, we believe
patient outcomes can be significantly improved by the development of novel diagnostic tests. Furthermore, an increasing number of women
are becoming aware of the importance of early detection, particularly in gynecologic diseases.

 Ovarian
Cancer Background

 Commonly
known as the "silent killer," ovarian cancer leads to nearly 13,000 deaths each year in the United States. In 2024, The American
Cancer Society ("ACS") estimated that nearly 20,000 new ovarian cancer cases were diagnosed, with the majority of patients
diagnosed in the late stages of the disease in which the cancer has spread beyond the ovary. Unfortunately, ovarian cancer patients in
the late stages of the disease have a poor prognosis, which leads to high mortality rates. According to the ACS, when ovarian cancer
is diagnosed at its earliest stage (stage 1), patients have up to a 93%, 5-year survival rate following surgery and/or chemotherapy.
The 5-year survival rate falls to as low as 31% for ovarian cancer patients diagnosed in the late-stages of the disease.

 While
the diagnosis of ovarian cancer in its earliest stages greatly increases the likelihood of long-term survival from the disease, another
factor that predicts clinical outcomes from ovarian cancer is the specialized training of the surgeon who operates on the ovarian cancer
patient. Numerous studies have demonstrated that treatment of malignant ovarian tumors by specialists such as gynecologic oncologists
coupled with specialist medical centers improves outcomes for women with these tumors. Published guidelines from the SGO and the ACOG
recommends referral of women with malignant ovarian tumors to specialists. Accordingly, there is a clinical need for a diagnostic test
that can provide adequate predictive value to stratify patients with a pelvic mass into those with a high-risk of invasive ovarian cancer
versus those with a low-risk of ovarian cancer, which is essential for improving overall survival in patients with ovarian cancer. The
goal is to catch the mass early before it becomes late-stage cancer.

 Although
adnexal masses are relatively common, malignant tumors are less so. Studies have indicated that the prevalence of simple ovarian cysts
in women 55 years of age and older can be as high as 14%. Adnexal masses are thought to be even more common in premenopausal women. For
instance, a University of Kentucky ovarian cancer screening study found that the rate of postmenopausal women with persistently abnormal
ultrasound findings requiring surgery was 1.4%. According to 2020 U.S. census data, there are 42.6 million women between the ages of
50 and 70 in the U.S., suggesting that there are nearly 600,000 suspicious adnexal masses in this segment alone. When managing an adnexal
mass, physicians will either take a surgical management approach or a clinical management approach. Patients that do require surgical
management could potentially benefit from the use of the Ova1Plus workflow. Patients not referred for surgical intervention may benefit
from the use of OvaWatch to confirm the low risk of malignancy of a mass that was determined to be indeterminate or benign by initial
clinical assessment.

 The
ACOG Ovarian Cancer Guidelines and the SGO guidelines help physicians evaluate adnexal masses for malignancy. These guidelines take into
account menopausal status, CA125 levels, and physical and imaging findings. However, these guidelines have notable shortcomings because
of their reliance on diagnostics with certain weaknesses. Most notably, studies have shown that the CA125 blood test, which is cleared
by the FDA for the monitoring for recurrence of ovarian cancer only, is negative in up to 31% of early-stage ovarian cancer cases. Moreover,
CA125 can be elevated in numerous conditions and diseases other than ovarian cancer, including menstruation, benign ovarian masses, liver
disease, endometriosis, pelvic inflammatory disease, pregnancy and uterine fibroids.

 These
shortcomings limit the CA125 blood test's utility in distinguishing benign from malignant ovarian tumors or for use in detection
of early-stage ovarian cancer.

 Transvaginal
ultrasound is another diagnostic modality used with patients with ovarian masses. Attempts at defining specific morphological criteria
that can aid in a benign versus malignant diagnosis have led to the morphology index and the risk of malignancy index, with reports of
40-70% predictive value. However, ultrasound interpretation can be variable and dependent on the experience of the operator. Accordingly,
the ACOG and SGO guidelines indicate that transvaginal ultrasounds are rarely conclusive in identifying early-stage ovarian cancer and
malignancy in pre-menopausal women. Efforts to improve detection of cancer by lowering the cutoff for CA125 (the "Modified ACOG/SGO
Guidelines") provide only a modest benefit, since CA125 is absent in about 20% of epithelial ovarian cancer cases and is poorly
detected in early-stage ovarian cancer overall.

 ACOG
practice bulletin 174 (November 2016) states the following regarding our Ova1-branded product "The multivariate index assay has
demonstrated higher sensitivity and negative predictive value for ovarian malignancy when compared with clinical impression and CA 125
alone."

 The
ovarian cancer information page on American Cancer Society's website indicates that:

 For
women who have an ovarian tumor, a test called Ova1 can measure the levels of 5 proteins in the blood. The levels of these proteins,
when looked at together, are used to determine whether a woman's tumor should be considered low-risk or high-risk. If the tumor
is labeled 'low-risk' based on this test, the woman is not likely to have cancer. If the tumor is considered 'high-risk,'
the woman is more likely to have a cancer and should see a specialist (a gynecologic oncologist). This test is NOT a screening test and
it is NOT a test to decide if you should have surgery or not- it is meant for women who have an ovarian tumor where surgery has
been decided but have not yet been referred to a gynecologic oncologist.

is committed to developing diagnostic tools for women of all ages, races and ethnicities. In 2019, two studies were released indicating
superior clinical performance of Ova1 over CA125 and Ova1 over CA125, HE4 and Risk of Ovarian Malignancy Algorithm ("ROMA")
in African American women. In 2022, another study was released indicating superior clinical performance of Ova1 over CA125 in Filipino
women.

 Commercialization
and Distribution

 We
market and distribute our products through 1) a national sales team, 2) the Aspira Synergy cloud-based technology transfer platform,
and 3) various commercial partnerships.

 Starting
in 2014, we offered Ova1 via Aspira Labs. In March 2015, we entered into a commercial agreement with Quest Diagnostics. Pursuant to this
agreement, as subsequently amended, all Ova1 U.S. testing services for Quest Diagnostics customers were transferred to Aspira's
wholly-owned subsidiary, Aspira Labs and Quest Diagnostics has continued to provide blood draw and logistics support by transporting
specimens from its clients to Aspira Labs for testing in exchange for a market value fee. In 2022, the agreement was amended to include
OvaWatch testing services. In October 2022, we launched a co-marketing and distribution collaboration with BioReference, as a new channel
for volume growth. Under terms of the agreement, the Aspira and BioReference and sales teams collaborate to sell Ova1Plus to gynecologists
and other women's healthcare providers nationwide. In November 2024, Aspira and BioReference announced the expansion of the sale
team collaboration to include OvaWatch.

 Customers

 In
the United States, our clinical customer base includes physicians (including women's care super-groups), physician office laboratories
and national and regional laboratories. Both within and outside the United States, our customer specimens are sent directly to us, and
we either bill third party payers or bill clients through client bill arrangements. We also offer access to our Ova1 and Overa assays
via our decentralized technology transfer relationships established between us and authorized distributors.

 Research
and Development

 Our
research and development efforts center on the discovery and validation of biomarkers and the combinations of biomarkers with other "omics"
that can be developed into diagnostic assays. We have done this predominantly through collaborations we have established with academic
institutions such as the Johns Hopkins University School of Medicine, the University of Texas, M.D. Anderson Cancer Center, Harvard's
Dana-Farber Cancer Institute, Brigham & Women's Hospital and Medical University of Lodz. In addition, we actively seek collaborations
and initiate dialog with clinical academics and other organizations, in order to generate publications, intellectual property or test
development in broader areas of gynecologic oncology and other gynecologic diseases.

 Our
research and development efforts are detailed in the "Product Pipeline" section above.

 In
2019, two studies identified a disparity in diagnosis for African American women and demonstrated that Ova1 has superior sensitivity
for detection in this population over CA125 or ROMA. In 2022, another study demonstrated the superiority of Overa over CA125 in Filipino
women.

 In
2022 and early 2023, two OvaWatch peer-reviewed validations were published. The first, "Analytical Validation of a Deep Neural
Network Algorithm for the Detection of Ovarian Cancer," validates the OvaWatch algorithm in the detection of ovarian cancer and
demonstrates the potential of OvaWatch in accurately assessing the risk of ovarian malignancy in patients with pelvic masses. Ovarian
cancer is the deadliest gynecologic cancer, with most cases being diagnosed at late stage. Early detection of ovarian cancer is key to
helping to reduce mortality; however, other current non-invasive risk assessment measures on the market vary in their usefulness. The
other paper, "Validation of Deep Neural Network-based Algorithm Supporting Clinical Management of Adnexal Mass," presents
findings from the multi-site clinical study of our new assay, OvaWatch, describing real-world evidence supporting the use of OvaWatch
for the clinical management of adnexal masses.

 On
May 7, 2024, we announced the publication of two peer-reviewed manuscripts, "Ovarian Cancer Surgical Consideration is Markedly
Improved by the Neural Network Powered-MIA3G Multivariate Index Assay" and "Neural Network-derived Multivariate Index Assay
Demonstrates Effective Clinical Performance in Longitudinal Monitoring of Ovarian Cancer Risk." See "Studies and Publications"
above for additional information.

 Commercial
Operations

 We
have a commercial infrastructure, including sales and marketing and reimbursement expertise. We also operate Aspira Labs, a CLIA certified
clinical laboratory in Austin, Texas. Our sales representatives work to identify opportunities for educating general gynecologists and
gynecologic oncologists on the benefits of Ova1. In February 2015, Aspira received ISO 13485:2003 certification for our quality management
system from the British Standards Institution (BSI), one of the world's leading certification bodies. We currently hold CE marks
for Ova1 and Overa. We are targeting markets outside of the United States now that we have Overa cleared on the Roche cobas platform,
which is available globally.

 24,305 OvaSuite tests were performed in 2024 compared to 23,990 in 2023. In 2024, we continued to increase sales through our commercial
team, including field sales, strategic alliances, and inside sales representatives. As awareness of our product continues to build, these
representatives are focused on efforts that will have a positive impact on regional payers and create positive payer coverage decisions
by driving physician demand. They work with local key opinion leaders and meeting with medical directors to discuss the clinical need,
our technology solutions package and increasing patient experience and cases studies showing the positive outcomes utilizing OvaSuite.

 We
believe OvaWatch will continue to have a significant impact on the ordering behavior of physicians with respect to our ovarian
cancer blood tests. OvaWatch was developed for use with women with adnexal masses that have been identified as either benign or
indeterminate through initial clinical assessment. It is estimated that physicians see more than three times as many women with
benign or indeterminate masses compared to women with masses that are planned for surgery. In addition, we believe that the OvaWatch
longitudinal monitoring test could further expand the patient population and the
ordering frequency of our ovarian cancer blood tests.

 Revenue
and Reimbursement

 In
the United States, revenue for diagnostic tests comes from several sources, including third-party payers such as insurance companies,
government healthcare programs, such as Medicare and Medicaid, client bill accounts and patients. Novitas Solutions, our Medicare Administrative
Contractor, covers and reimburses for Ova1 tests performed based on an LCD in its jurisdiction. Due to Ova1 tests being performed at
Aspira Labs in Texas, an LCD from Novitas Solutions provides national coverage for patients enrolled in Medicare as well as Medicare
Advantage health plans. Aspira Labs also bills third-party commercial and other government payers as well as client bill accounts and
patients for Ova1. Through December 31, 2024, Aspira's product and related services revenue was primarily limited to revenue generated
by sales of Ova1 and OvaWatch.

 In
December 2013, the CMS made its final determination and authorized Medicare contractors to set prices for Multianalyte Assays with Algorithmic
Analyses ("MAAA") test CPT codes when they determine it is payable. In late 2016, Ova1 was included on the list of clinical
diagnostic laboratory test procedure codes as one for which the CMS would require reporting of private payer rates as part of the implementation
of Protecting Access to Medicare Act of 2014 ("PAMA"). In November 2017, we announced that the CMS released the Final 2018
Clinical Laboratory Fee Schedule ("CLFS"), effective January 1, 2018. Under the fee schedule, the price for Ova1 (CPT code
81503) is $897. This rate was based on the median of private payer payments submitted to CMS by companies, including Aspira Labs, as
part of the market-based payment reform mandated through PAMA. The rate was scheduled to be in effect for a three-year term from January
2018 through December 2020. This rate is now extended through 2025. In 2024, CMS announced that it would continue to delay the period
during which rates would be evaluated for another year. Therefore, we will not be responsible for providing reimbursement rates until
January 2026. There are no assurances that reimbursement rates will not be changed.

 Despite
gains in positive medical policy coverage and contract agreements, insurance coverage and patient bills remain a concern to the physician
and can disrupt the ordering pattern of a provider who is supportive of our products. We have instituted a "Patient Transparency
Program" to assist with this process by proactively assessing insurance and educating patients on testing costs prior to testing
being performed. Legislation to expand access to multi-cancer early detection technology under Medicare was reintroduced in the current
legislative session. HR 2407, introduced in March 2023, and S 1085, introduced in June 2023, would create the authority for CMS to cover
blood-based multi-cancer early detection tests once approved by FDA and shown to have clinical benefit.

 We
have a comprehensive reimbursement plan for Ova1 and OvaWatch, and have targeted third-party payers, Medicare, Medicare Advantage, State
Medicaid and Managed Medicaid plans for coverage and reimbursement. In April 2023 we began billing OvaWatch with our newly awarded Proprietary
Laboratory Analyses ("PLA Code") 0375U. Since we began billing OvaWatch with the PLA Code, our reimbursement has been more
in-line with historical Ova1Plus experience, resulting in the OvaWatch average unit price ("AUP") of $362 for the year ended
December 31, 2024.

 Ova1
is considered medically necessary in the Lab Management Guidelines for one of the largest lab benefit management companies who works
with payers to ensure adherence to clinical guidelines. We continue to make gains toward reimbursement for OvaWatch as we build clinical
evidence to support coverage.

 In
February 2023, we signed a contract with a national commercial payer which provides patient coverage for Ova1 and OvaWatch beginning
in April 2023. Further, CMS approved the crosswalk of the fee to be paid for OvaWatch to the fee paid for Ova1. Effective January 1,
2024, we have been reimbursed at a rate of $897 for all OvaWatch and Ova1 tests processed for Medicare patients meeting applicable coverage
requirements.

 In
addition, the United States and some foreign jurisdictions are considering or have enacted a number of legislative and regulatory proposals
to change the healthcare system in ways that could affect our ability to sell our products profitably. Among policy makers and payers
in the United States and elsewhere, there is significant interest in promoting changes in healthcare systems with the stated goals of
containing healthcare costs, improving quality or expanding access.

 Further,
other legislative changes have been proposed and adopted since the Affordable Care Act was enacted. For example, the Budget Control Act
of 2011, among other things, included reductions to CMS payments to providers of 2% per fiscal year, which went into effect on April
1, 2013 and, due to subsequent legislative amendments to the statute, will remain in effect until 2032 unless additional congressional
action is taken.

 The
Nancy Gardner Sewell Medicare Multi-Cancer Early Detection Screening Coverage Act and the Medicare Multi-Cancer Early Detection Screening
Coverage Act are bills that modernize the Medicare program and create a benefit category for MCED tests, which allow the CMS to initiate
an evidence-based coverage process for multi-cancer tests upon approval by the FDA. The House bill (H.R. 2407) was introduced with bipartisan
support on March 30, 2023 and its Senate companion (S. 2085) was introduced on June 22, 2023.

 There
may be additional health reform initiatives by legislators at both the federal and state levels, regulators and third-party payers to
reduce costs while expanding individual healthcare benefits. Certain of these changes could impose additional limitations on the rates
we will be able to charge for our current and future products or the amounts of reimbursement available for our current and future products
from governmental agencies or third-party payers.

 Biomarker
legislation continues to gain momentum on the state level with many states enacting legislation requiring coverage in both public and
private insurance plans. Additionally, more states are evaluating legislation and introduced biomarker access bills in 2024.

 Competition

 The
diagnostics industry in which we operate is competitive and evolving. There is intense competition among healthcare, biotechnology and
diagnostics companies attempting to discover candidates for potential new diagnostic products. These companies may:

 develop
 new diagnostic products in advance of us or our collaborators;

 develop
 diagnostic products that are more effective or cost-effective than those developed by us or our collaborators;

 obtain
 regulatory clearance or approval of their diagnostic products more rapidly than us or our collaborators; or

 obtain
 patent protection or other intellectual property rights that would limit our or our collaborators' ability to develop and commercialize,
 or a customers' ability to use our or our collaborators' diagnostic products.

 We
compete with companies in the United States and abroad that are engaged in the development and commercialization of novel biomarkers
that may form the basis of novel diagnostic tests. These companies may develop products that are competitive with and/or perform the
same or similar functions as the products offered by us or our collaborators, such as biomarker specific reagents or diagnostic test
kits. Also, clinical laboratories may offer testing services that are competitive with the products sold by us or our collaborators.
For example, a clinical laboratory can either use reagents purchased from manufacturers other than us or use its own internally developed
reagents to make diagnostic tests. If clinical laboratories make tests in this manner for a particular disease, they could offer testing
services for that disease as an alternative to products sold by us used to test for the same disease. The testing services offered by
clinical laboratories may be easier to develop and market than test kits developed by us or our collaborators because the testing services
are not subject to the same clinical validation requirements that are applicable to FDA-cleared or approved diagnostic test kits.

 Fujirebio
Diagnostics sells ROMA. ROMA combines two tumor markers and menopausal status into a numerical score using a publicly available algorithm.
This test has the same intended use and precautions as Ova1. ROMA is currently marketed as having utility limited to epithelial ovarian
cancers, which accounts for 80% of ovarian malignancies. Based upon the results of studies done in 2013 and 2019, we believe that Ova1
has superior sensitivity when compared to the Fujirebio Diagnostics test.

 In
addition, competitors such as AOA Dx, ClearNote Health, Cleo Diagnostics and Mercy BioAnalytics, and others have publicly disclosed that
they have been or are currently working on ovarian cancer diagnostic assays. Exact Sciences, Grail and others are working on multi-cancer
early diagnostic tests that include ovarian cancer detection. Academic institutions periodically report new findings in ovarian cancer
diagnostics that may have commercial value.

 A
number of diagnostic and academic organizations have announced plans or published studies related to the development of a non-invasive
diagnostic tool for the identification of endometriosis. If successful, the product may be competitive with our endometriosis offerings.
Competitors include, but are not limited to, Afynia, DotLab, Endodiag, HERA Biotech Heranova, Proteomics International and Ziwig. We
believe our experience developing multi-biomarker assays, particularly those focused on gynecologic diseases and pelvic masses, as well
as our experienced women's health field sales team and our focus on developing a clinical assay in our CLIA laboratory environment,
is a significant competitive advantage.

 Intellectual
Property Protection

 Our
intellectual property includes federally registered trademarks and service marks as well as federally pending trademark and service mark
applications for our product and service offerings, and a portfolio of owned, co-owned or licensed patents and patent applications. As
of the date of the filing of the Form 10-K, our clinical diagnostics patent portfolio included 18 issued United States patents, 8
pending United States patent applications and numerous pending patent applications and issued patents outside the United States. These
patents and patent applications are directed to diagnostic technologies.

 Manufacturing

 We
are the manufacturer of FDA cleared products Ova1 and Overa, which are part of the Ova1Plus workflow. We also perform OvaWatch as an
LDT. The component assays use purchased reagents. Because we do not directly manufacture the component assays, we are required to maintain
supply agreements with manufacturers of each of the assays. As part of our quality systems, reagent lots for these assays are tested
to ensure they meet specifications required for inclusion. Only reagent lots determined by us as having met these specifications are
permitted for use in our testing. Our principal supplier for the component reagents is Roche Diagnostics Corporation.

 Environmental
Matters

 Medical
Waste

 We
are subject to licensing and regulation under federal, state and local laws relating to the handling and disposal of medical specimens
and hazardous waste as well as relating to the safety and health of laboratory employees. Aspira Labs is operated in material compliance
with applicable federal and state laws and regulations relating to disposal of all laboratory specimens. We utilize outside vendors for
disposal of specimens. We cannot eliminate the risk of accidental contamination or discharge and any resultant injury from these materials.
Federal, state and local laws and regulations govern the use, manufacture, storage, handling and disposal of these materials. We could
be subject to fines, penalties and damages claims in the event of an improper or unauthorized release of, or exposure of individuals
to, hazardous materials. In addition, claimants may sue us for injury or contamination that results from our use, or the use by third
parties, of these materials, and our liability may exceed our total assets. Compliance with environmental laws and regulations is expensive,
and current or future environmental regulations may impair our research, development or production efforts.

 Occupational
Safety

 In
addition to its comprehensive regulation of safety in the workplace, the Federal Occupational Safety and Health Administration has established
extensive requirements relating to workplace safety for healthcare employers whose workers may be exposed to blood-borne pathogens such
as HIV and the hepatitis virus. These regulations, among other things, require work practice controls, protective clothing and equipment,
training, medical follow-up, vaccinations and other measures designed to minimize exposure to chemicals and transmission of blood-borne
and airborne pathogens. Although we believe that we have complied in all material respects with such federal, state and local laws, failure
to comply could subject us to denial of the right to conduct business, fines, criminal penalties and other enforcement actions.

 Specimen
Transportation

 Regulations
of the Department of Transportation, the International Air Transportation Agency, the Public Health Service and the Postal Service apply
to the surface and air transportation of clinical laboratory specimens. Although we believe that we have complied in all material respects
with such federal, state and local laws, failure to comply could subject us to denial of the right to conduct business, fines, criminal
penalties and other enforcement actions.

 Government
Regulation

 FDA
Regulation of Medical Devices

 In
the U.S., medical devices, including IVD products ("IVDs"), are subject to extensive regulation by the FDA, under the Federal
Food, Drug, and Cosmetic Act (the "FDC Act"), and its implementing regulations, and certain other federal and state statutes
and regulations. The laws and regulations govern, among other things, the design, manufacture, storage, recordkeeping, approval, labeling,
promotion, post-approval monitoring and reporting, distribution and import and export of medical devices, including IVDs. IVDs are a
type of medical device and include reagents and instruments used in the diagnosis or detection of diseases or conditions. Predictive,
prognostic, and screening tests can also be IVDs. Failure to comply with applicable requirements may subject a device and/or its manufacturer
to a variety of administrative and judicial sanctions, such as FDA refusal to approve pending pre-market approval applications ("PMAs")
or other applications, issuance of warning letters or untitled letters, mandatory product recalls, import detentions, civil monetary
penalties, and/or judicial sanctions, such as product seizures, injunctions, and criminal prosecution.

 The
FDC Act classifies medical devices into one of three categories based on the risks associated with the device and the level of control
necessary to provide reasonable assurance of safety and effectiveness. Class I devices are deemed to be low risk and are subject only
to the general regulatory controls. Class II devices are moderate risk. They are subject to general controls and may also be subject
to special controls. Class III devices are generally the highest risk devices. They are required to obtain premarket approval and comply
with post-market conditions of approval in addition to general regulatory controls.

 Generally,
establishments that design, manufacture, re-label/re-package, process, and/or import devices are required to register their establishments
with the FDA. They also must provide the FDA with a list of the devices that they design, manufacture, re-label/re-package, process,
and/or import at their facilities.

 The
FDA enforces its requirements by market surveillance and periodic visits, both announced and unannounced, to inspect or re-inspect equipment,
facilities, laboratories and processes to confirm regulatory compliance. These inspections may include the manufacturing facilities of
subcontractors that are device manufacturers. Following an inspection, the FDA may issue a report, known as a Form 483, listing instances
where the manufacturer has failed to comply with applicable regulations and/or procedures or, if observed violations are sufficiently
serious, a warning letter. If the manufacturer does not adequately respond to a Form 483 or warning letter, the FDA make take enforcement
action against the manufacturer or impose other sanctions or consequences, which may include:

 cease
 and desist orders;

 injunctions
 or consent decrees;

 civil
 monetary penalties;

 recall,
 detention or seizure of products;

 operating
 restrictions or partial or total shutdown of production facilities;

 refusal
 of or delay in granting requests for 510(k) clearance, de novo classification, or premarket approval of new products or modified
 products;

 withdrawing
 510(k) clearances, de novo classifications, or premarket approvals that are already granted;

 refusal
 to grant export approval or export certificates for devices; and

 criminal
 prosecution.

 The
FDA also monitors advertising and promotion of regulated devices, including IVDs, to ensure that all promotion of the product is consistent
with the device's intended use reflected in the FDA-cleared and/or -approved labeling. If the device manufacturer promotes the
product in violation of advertising and promotion rules established in the FDC Act and FDA's implementing regulations, FDA may
issue a so-called "Untitled Letter" requiring the manufacturer to amend and/or remove promotion of its product that is not
compliant with such rules. Additionally, FDA may take any of the enforcement actions outlined above.

 Pre-Market
Authorization and Notification

 Unless
subject to an exemption, medical devices require prior FDA authorization before they may be commercially marketed. Devices can be legally
sold within the U.S. only if the FDA has: (i) approved a pre-market approval application prior to marketing, generally applicable to
most Class III devices; (ii) cleared the device in response to a 510(k) premarket notification submission ("510(k)"), generally
applicable to Class I and II devices; or (iii) reclassified the device pursuant to the de novo classification process, available
for novel low or moderate risk devices. PMA applications, 510(k) premarket notifications, and de novo classification requests
require payment of substantial user fees that are increased each fiscal year.

 Ova1,
the first FDA-authorized blood test for the pre-operative assessment of ovarian masses, was authorized by the FDA in September 2009 under
the de novo classification pathway. We received 510(k) clearance for Overa, our second-generation biomarker panel in March 2016.

 510(k)
Premarket Notification

marketing in the U.S. for most Class II and a limited number of Class I devices typically follows the 510(k) premarket notification pathway.
To obtain 510(k) clearance, a manufacturer must submit a premarket notification demonstrating that the proposed device is substantially
equivalent to a legally marketed device, referred to as the "predicate device." A predicate device may be a previously 510(k)
cleared device or a Class III device that was in commercial distribution before May 28, 1976 for which the FDA has not yet called for
PMA applications, or a product previously placed in Class II or Class I through the de novo or other classification process. The
manufacturer must show that the proposed device has the same intended use as the predicate device, and it either has the same technological
characteristics, or it is shown to be equally safe and effective and does not raise different questions of safety and effectiveness as
compared to the predicate device. A 510(k) may need to be supported by clinical data.

 FDA
has a user fee goal to apply no more than 90 calendar review days to 510(k) submissions. During the process, FDA may issue an Additional
Information request, which stops the FDA's review clock. The applicant has 180 days to respond. Therefore, the total review time
could be up to 270 days, although it can take longer.

 After
a device receives 510(k) clearance, any modification that could significantly affect its safety or effectiveness, or that would constitute
a major change in its intended use, requires a new 510(k) clearance or could require a PMA approval or de novo classification.
The FDA requires each manufacturer to make this determination in the first instance, but the FDA can review any such decision. If the
FDA disagrees with a manufacturer's decision not to seek a new 510(k) clearance for the modified device, the agency may require
the manufacturer to seek 510(k) clearance, de novo classification, or PMA approval. The FDA also can require the manufacturer
to cease marketing and/or recall the modified device until 510(k) clearance or PMA approval is obtained.

 De
Novo Classification

 Devices
of a new type that the FDA has not previously classified based on risk are automatically classified into Class III regardless of the
level of risk they pose. To avoid requiring PMA review of novel low-to moderate-risk devices classified in Class III by operation of
law, Congress enacted a provision that allows the FDA to classify a novel low-to moderate-risk device into Class I or II in the absence
of a predicate device that would support 510(k) clearance. The FDA evaluates the safety and effectiveness of devices submitted for review
under the de novo pathway, and devices determined to be Class II through this pathway can serve as predicate devices for future
510(k) applicants. The de novo pathway generally requires clinical data. As part of the de novo process FDA will establish
special controls to help ensure the safety and effectiveness of the device.

 FDA
has a user fee goal to review a de novo request in 150 calendar review days. During the process, FDA may issue an Additional Information
request, which stops the FDA's review clock. The applicant has 180 days to respond. Therefore, the total review time could be as
long as 330 days, although it can take longer.

 PMA
Approval

 A
Class III product not eligible for either 510(k) clearance or de novo classification must follow the PMA approval pathway.

 Results
from clinical trials are required for each indication for which FDA approval is sought. After completion of the required clinical testing,
a PMA including the results of all non-clinical, clinical, and other testing and information relating to the product's marketing
history, design, labeling, manufacture, and controls, is prepared and submitted to the FDA.

 The
PMA approval process is generally more expensive, rigorous, lengthy, and uncertain than the 510(k) premarket notification process and
 de novo classification process and requires proof of the safety and effectiveness of the device to the FDA's satisfaction.
As part of the PMA review, the FDA will typically inspect the manufacturer's facilities for compliance with Quality System Regulation
("QSR"), requirements, which impose elaborate testing, control, documentation and other quality assurance procedures. FDA
has a user fee goal to review a PMA in 180 calendar review days, if the submission does not require advisory committee input, or 320
review days if the submission does require advisory committee input. During the process, FDA may issue a major deficiency letter, which
stops the FDA's review clock. The applicant has up to 180 days to respond. Therefore, the total review time could be up to 360
days, if the submission does not require advisory committee input, or 500 days if the submission does require advisory committee input,
although it could take longer.

 If
the FDA's evaluation of the PMA application is favorable, the FDA will issue a PMA for the approved indications, which can be more
limited than those originally sought by the manufacturer. The PMA can include post-approval conditions that the FDA believes necessary
to ensure the safety and effectiveness of the device including, among other things, post-approval studies and restrictions on labeling,
promotion, sale and distribution. Failure to comply with the conditions of approval can result in material adverse enforcement action,
including the loss or withdrawal of the approval and/or placement of restrictions on the sale of the device until the conditions are
satisfied.

 Even
after approval of a PMA, a new PMA or PMA supplement may be required in the event of a modification to the device, its labeling or its
manufacturing process. Supplements to a PMA often require the submission of the same type of information required for an original PMA,
except that the supplement is generally limited to that information needed to support the proposed change from the product covered by
the original PMA.

Trials

 Generally,
data from at least one clinical trial is required to support a PMA application. Evidence from clinical studies also typically is included
in a request for de novo classification and, less frequently, in a 510(k) premarket notification. Clinical trials may also be
conducted or continued to satisfy post-approval requirements for devices with PMAs. For significant risk investigational device studies,
the FDA regulations require that human clinical investigations conducted in the U.S. be approved under an investigational device exemption
("IDE"), which must become effective before clinical testing may commence. A nonsignificant risk investigational device study
does not require FDA approval of an IDE, although it does need to comply with some elements of the IDE regulations. Some studies of IVDs
are entirely exempt from the IDE requirements. In some cases, one or more smaller IDE studies may precede a pivotal clinical trial intended
to demonstrate the safety and efficacy of the investigational device. A 30-day waiting period after the submission of each IDE is required
prior to the commencement of clinical testing in humans. If the FDA disapproves the IDE within this 30-day period, the clinical trial
proposed in the IDE may not begin. Clinical trials of IVDs that meet certain regulatory criteria are exempt from the IDE regulations.

 An
IDE application must be supported by appropriate data, such as animal and laboratory test results, showing that it is safe to test the
device in humans and that the testing protocol is scientifically sound. The IDE application must also include a description of product
manufacturing and controls, and a proposed clinical trial protocol. The FDA typically grants IDE approval for a specified number of patients
to be treated at specified study centers. During the study, the sponsor must comply with the FDA's IDE requirements for investigator
selection, trial monitoring, reporting, and record keeping. The investigators must obtain patient informed consent, follow the investigational
plan and study protocol, control the disposition of investigational devices, and comply with reporting and record keeping requirements.
Prior to granting PMA approval, the FDA typically inspects the records relating to the conduct of the study and the clinical data supporting
the PMA application for compliance with IDE requirements.

trials must be conducted: (i) in compliance with federal regulations; (ii) in compliance with good clinical practice ("GCP"),
an international standard intended to protect the rights and health of patients and to define the roles of clinical trial sponsors, investigators,
and monitors; and (iii) under protocols detailing the objectives of the trial, the parameters to be used in monitoring safety, and the
effectiveness criteria to be evaluated. Clinical trials are typically conducted at geographically diverse clinical trial sites and are
designed to permit the FDA to evaluate the overall benefit-risk relationship of the device and to provide adequate information for the
labeling of the device when considering whether a device satisfies the statutory standard for commercialization. Clinical trials, for
both significant and nonsignificant risk device studies, as well as exempt IVD studies, must be approved by an institutional review board
("IRB"), an appropriately constituted group that has been formally designated to review and monitor biomedical research involving
human subjects and which has the authority to approve, require modifications in, or disapprove research to protect the rights, safety,
and welfare of the human research subject. Informed consent of patients participating in the study generally must be obtained before
they may participate in the study.

 The
FDA may order the temporary, or permanent, discontinuation of a clinical trial at any time, or impose other sanctions, if it believes
that the clinical trial either is not being conducted in accordance with the FDA requirements or presents an unacceptable risk to the
clinical trial patients. An IRB may also require the clinical trial it has approved to be halted, either temporarily or permanently,
for failure to comply with the IRB's requirements, or may impose other conditions or sanctions.

 Although
the QSR does not fully apply to investigational devices, the requirement for controls on design and development does apply to devices
subject to FDA's IDE regulations. The sponsor also must manufacture the investigational device in conformity with the quality controls
described in the IDE application and any conditions of IDE approval that the FDA may impose with respect to manufacturing.

 Post-Market
Requirements

 After
a device is placed on the market, numerous general regulatory controls apply. These include: the QSR (which requires manufacturers to
have a quality policy and procedures to ensure that devices are manufactured and records maintained in a prescribed manner with respect
to manufacturing, testing, complaint handling, and record keeping), labeling regulations, the medical device reporting regulations (which
require that manufacturers report to the FDA if their device may have caused or contributed to a death or serious injury or malfunctioned
in a way that would likely cause or contribute to a death or serious injury if it were to recur), and reports of corrections and removals
regulations (which require manufacturers to report recalls or removals and field corrections to the FDA if initiated to reduce a risk
to health posed by the device or to remedy a violation of the FDC Act if that violation may present a risk to health). Failure to properly
identify reportable events or to file timely reports, as well as failure to comply with other regulatory requirements, can subject a
manufacturer to warning letters, recalls, or other sanctions and penalties.

 As
a manufacturer of IVDs, we are subject to regulatory oversight by the FDA under provisions of the FDC Act and regulations thereunder.
We are required to register and list our IVD products with the FDA and to comply with the applicable provisions of the QSR. We are required
to submit a medical device report whenever we receive information that reasonably suggests that one of our devices may have caused or
contributed to a death or serious injury, or where a malfunction has occurred that would be likely to cause or contribute to a death
or serious injury if the malfunction were to recur. As of the date of the filing of this Annual Report on Form 10-K, we have had zero
complaints that required us to submit a medical device report to FDA. Additionally, we are subject to inspection by the FDA. Further,
we are required to comply with FDA requirements for labeling and promotion.

 Marketing
and promotional activities for devices, and advertising of some restricted medical devices, are also subject to FDA oversight and must
comply with the statutory standards of the FDC Act, and the FDA's regulatory requirements. The FDA's oversight of marketing
and promotional activities encompasses, but is not limited to, direct-to-consumer advertising, healthcare provider-directed advertising
and promotion, sales representative communications to healthcare professionals and promotional activities involving electronic media.
The FDA also regulates industry-sponsored scientific and educational activities that make representations regarding product safety or
efficacy in a promotional context.

 Manufacturers
of medical devices are permitted to promote products solely for the uses and indications that are consistent with those set forth in
the approved or cleared product labeling. A number of enforcement actions have been taken against manufacturers that promote products
for "off-label" uses (i.e., uses that are not described in the approved or cleared labeling), including actions alleging
that claims submitted to government healthcare programs for reimbursement of products that were promoted for "off-label"
uses are in violation of the Federal False Claims Act or other federal and state statutes and that the submission of those claims was
caused by off-label promotion. The failure to comply with prohibitions on "off-label" promotion can result in significant
monetary penalties, suspension of sales of certain products, product recalls, civil or criminal sanctions, exclusion from participating
in federal healthcare programs, or other enforcement actions. Such wrongful conduct could also result in a corporate integrity agreement
with the U.S. government that imposes significant administrative obligations and costs.

 Violations
of the FDC Act relating to the inappropriate promotion of approved products may lead to investigations alleging violations of federal
and state healthcare fraud and abuse and other laws, as well as state consumer protection laws, which could, in turn, lead to additional
enforcement actions, including, but not limited to, injunctions, civil monetary penalties, and/or criminal penalties.

 For
a PMA or Class II 510(k) or de novo device, the FDA also may impose post-market conditions of approval, such as testing, surveillance,
or other measures to monitor the effects of an approved or cleared product. The FDA may place conditions on a PMA-approved device that
could restrict the distribution or use of the product. In addition, quality-control, manufacture, packaging, and labeling procedures
must continue to conform to the QSR after approval and clearance, and manufacturers are subject to periodic inspections by the FDA. Accordingly,
manufacturers must continue to expend time, money, and effort in the areas of production and quality control to maintain compliance with
the QSR. The FDA may withdraw product approvals or recommend or require product recalls if a company fails to comply with regulatory
requirements.

studies to support FDA marketing authorization of new IVD products or new indications for already-authorized IVD products must be conducted
in accordance with the applicable FDA regulations.

 We
also may be required to conduct post-market surveillance of medical devices as a condition of granting marketing authorization. With
respect to Ova1, the FDA required us to perform post-market surveillance to gather additional data regarding test performance. This study
has been completed.

Laboratory Improvement Amendments of 1988

laboratories operating in or testing specimens from the U.S. are subject to CLIA, and related federal and state regulations, which provide
for regulation of laboratory testing. Any customers using IVDs for clinical use in the United States will be regulated under CLIA, which
establishes quality standards for all laboratory testing to ensure the accuracy, reliability and timeliness of patient test results regardless
of where the test was performed. In particular, these regulations mandate that clinical laboratories must be certified by the federal
government or a federally approved accreditation agency or must be located in a state that has been deemed exempt from CLIA requirements
because the state has in effect laws that provide for requirements equal to or more stringent than CLIA requirements. Moreover, these
laboratories must meet quality assurance, quality control and personnel standards, and they must undergo proficiency testing and inspections.
The CLIA standards applicable to clinical laboratories are based on the complexity of the method of testing performed by the laboratory,
as deemed by FDA, which range from "waived" to "moderate complexity" to "high complexity."

 Our
clinical laboratory activities are subject to CLIA and related state clinical laboratory laws. In June 2014, we launched a clinical laboratory,
Aspira Labs. Aspira Labs holds a CLIA Certificate of Accreditation and a state laboratory license or permit in California, Maryland,
New York, Pennsylvania, and Rhode Island. We are subject to periodic surveys and inspections to maintain our CLIA certification, and
such certification is also required to obtain payment from Medicare, Medicaid and certain other third-party payers.

 Laboratory
Developed Tests

 The
FDA considers LDTs to be tests that are designed, developed, validated and used within a single laboratory. LDTs are performed using
a variety of laboratory instruments and reagents and may also incorporate FDA-authorized IVDs that the laboratory modifies in some way
and validates for its new use. The FDA has historically taken the position that it has the authority to regulate LDTs as medical devices
under the FDC Act, but it has generally exercised enforcement discretion with regard to LDTs. This means that even though the FDA believes
it can impose regulatory requirements on LDTs, such as requirements to obtain premarket approval or clearance of LDTs, it has generally
chosen not to enforce those requirements.

 On
May 6, 2024, the FDA published a final rule amending the definition of an IVD device to include IVDs manufactured by a clinical laboratory,
effectively codifying its position that LDTs are IVDs and, therefore, that LDTs fall under FDA's regulatory authority. The final
rule also announced the FDA's intention to phase out its general enforcement discretion policy. Unless the rule is overturned by
a court or superseded by Congressional action, the medical device requirements for most LDTs will be phased in beginning on May 6, 2025.

 The
new rule implements a phased approach to ending FDA's policy of enforcement discretion for LDTs. The phased approach establishes
timelines for LDT sponsors to comply with different categories of FDA device regulations, and the clock started on the final rule's
publication date – May 6, 2024, the date to which all five phases were anchored. The phases are as follows: (1) LDTs are subject
to MDR, as well as adverse event reporting, one year after the final rule's publication date (i.e., May 6, 2025); (2) LDTs are
subject to registration/listing, labeling, and investigational use requirements two years after the final rule's publication date
(i.e., May 6, 2026); (3) LDTs are subject to Quality System regulations three years after the final rule's publication date (i.e.,
May 6, 2027); (4) high-risk LDTs are subject to premarket review (i.e., 510(k) clearance, de novo classification, or PMA, as applicable)
three-and-a-half years after the final rule's publication date (i.e., November 6, 2027), unless a premarket submission has been
received by the beginning of this stage in which case FDA intends to continue to exercise enforcement discretion for the pendency of
its review; and (5) mid- and low-risk LDTs are subject to premarket review (i.e., 510(k) clearance, de novo classification, or
PMA, as applicable) four years after the final rule's publication date (i.e., May 6, 2028), unless a premarket submission has been
received by the beginning of this stage in which case FDA intends to continue to exercise enforcement discretion for the pendency of
its review.

categories of LDTs will be subject to enforcement discretion with respect to some or all of these requirements. In total, the new rule
identifies eight types of LDTs for which it will continue to exercise enforcement discretion with respect to some or all regulatory requirements.
For example, the FDA will apply enforcement discretion to currently marketed LDTs that were first offered prior to May 6, 2024, with
respect to most quality system requirements and the requirement for premarket authorization if they are not modified or modified in only
limited ways. The FDA will similarly exercise enforcement discretion with respect to premarket authorization for LDTs approved by the
New York State Clinical Laboratory Evaluation Program. However, laboratories performing these tests are subject to all other requirements
outlined in FDA's phase-out policy, including, but not limited to, the requirement to submit the labeling for the LDT to FDA for
review. As outlined in the new rule, the FDA will also exercise enforcement discretion with respect to some or all regulatory requirements
for certain LDTs designed for rare, unmet, or specific needs, LDTs manufactured and performed within the VHA or DOD, so-called "1967-Type
LDTs," and forensic use LDTs.

 Legislative
proposals addressing the FDA's oversight of LDTs have been previously introduced. In March 2020, the VALID Act of 2020 was introduced
in the Senate, which proposes a risk-based regulatory framework for IVDs and LDTs and would require premarket approval for some in vitro
clinical tests. The VALID Act was reintroduced in July 2021 and again in March 2023; the prospects for enactment are uncertain. In March
2020, the Verified Innovative Testing in American Laboratories ("VITAL") Act of 2020 was introduced in the Senate, which
would expressly shift the regulation of LDTs from FDA to CMS. The VITAL Act was reintroduced in May 2021. Neither statute has been enacted.

 The
FDA's new rule, which establishes the phase-out policy for enforcement discretion with respect to LDTs, has been challenged in
two separate lawsuits – one brought in the District Court for the Eastern District of Texas and the other brought in the District
Court for the Southern District of Texas. As of the date of this filing, both cases are still pending.

 The
FDA has become increasingly active in addressing the regulation of software used to support clinical decision making. In 2016, the 21st
Century Cures Act (the "Cures Act"), among other things, amended the medical device definition in the FDC Act to exclude
certain software from FDA regulation, including clinical decision support ("CDS software"), that meets certain criteria.
CDS software is exempt from the medical device definition if it: (a) displays, analyzes or prints medical information about a patient
or other medical information; (b) is intended for the purpose of supporting or providing recommendations about a patient's care
to a healthcare professional ("HCP") user; and (c) provides sufficient information about the basis for the recommendations
to the HCP user, so that the HCP user does not rely primarily on any of the recommendations to make a clinical decision about an individual
patient; unless (d) the software function acquires, processes, or analyzes a medical image, a signal from an in vitro diagnostic device,
or a pattern or signal from a signal acquisition system.

 On
September 28, 2022, the FDA issued a final guidance document interpreting the Cures Act as it pertains to CDS software. Among other views
expressed, the final guidance stated that software functions that assess or interpret the clinical implications or clinical relevance
of a signal or pattern, such as those that process or analyze an electrochemical or photometric response generated by an assay and instrument
to generate a clinical test result, are not exempt from medical device regulation. The final guidance also stated that software functions
that generate risk probabilities or risk scores are not exempt because they necessarily provide a specific diagnostic, preventive, or
treatment output.

 Our
clinical laboratory activities are subject to CLIA and related state laws. In June 2014, we launched a clinical laboratory, Aspira Labs.
Aspira Labs holds a CLIA Certificate of Accreditation and a state laboratory license or permit in all states from which we accept specimens
that require a state-level laboratory license or permit, including California, Maryland, New York, Pennsylvania and Rhode Island. In
July 2021, we were granted a CLIA Certificate of Accreditation for our laboratory at our Connecticut office. We are subject to periodic
surveys and inspections to maintain our CLIA certification, and such certification is also required to obtain payment from Medicare,
Medicaid and certain other third-party payers.

 Foreign
Government Regulation of Our Products

 Medical device laws and regulations are in effect
in many of the countries in which we may do business outside the United States. These range from comprehensive device approval requirements
for some or all of our potential future medical device products, to requests for product data or certifications. The number and scope
of these requirements are increasing. In addition, products which have not yet been cleared or approved for domestic commercial distribution
may be subject to the FDA Export Reform and Enhancement Act of 1996. Each country also maintains its own regulatory review process, tariff
regulations, duties and tax requirements, product standards, and labeling requirements. In February 2015, Aspira also received ISO 13485:2003
certification for our quality management system from the British Standards Institution (BSI), one of the world's leading certification
bodies. In March 2015, Ova1 was CE marked, a requirement for marketing the test in the European Union. In October 2015, we announced
registration of the CE mark for and clearance to market Overa in the European Union.

 Privacy
and Security of Health Information

 The
federal Health Insurance Portability and Accountability Act of 1996, or HIPAA, as amended by the Health Information Technology for Economic
and Clinical Health Act, or HITECH, and final omnibus rules, were issued by HHS to protect the privacy and security of protected health
information used or disclosed by health care providers, such as us. HIPAA also regulates standardization of data content, codes and formats
used in health care transactions and standardization of identifiers for health plans and providers. Penalties for violations of HIPAA
regulations include civil and criminal penalties. In addition to federal privacy regulations, a number of state and international laws
govern confidentiality of health information.

 Health
Care Fraud and Abuse

 The
federal Anti-Kickback Statute makes it a felony for a provider or supplier, including a laboratory, to knowingly and willfully offer,
pay, solicit or receive remuneration, directly or indirectly, in order to induce business that is reimbursable under any federal health
care program, including Medicare and Medicaid. A violation of the federal Anti-Kickback Statute may result in imprisonment for up to
five years and/or criminal fines of up to $250,000 for an individual. Companies may be criminally fined up to $500,000 and may also be
subject to civil assessments and exclusion from participation in Medicare, Medicaid, and other federal health care programs.

 Actions
that violate the federal Anti-Kickback Statute may also be subject to liability under the Federal False Claims Act, which prohibits knowingly
presenting or causing to be presented a false or fraudulent claim for payment to the U.S. Government. Although the federal Anti-Kickback
Statute applies only to federal health care programs, a number of states have enacted statutes substantially similar to the federal Anti-Kickback
Statute pursuant to which similar types of prohibitions are made applicable to all other health plans and third-party payors.

 The
Eliminating Kickbacks in Recovery Act (EKRA) makes it a federal crime to knowingly and willfully solicit or receive any remuneration,
directly or indirectly, in return for referring a patient or patronage any laboratory. EKRA also prohibits paying or offering any remuneration
directly or indirectly: (A) to induce a referral of an individual to a laboratory; or (B) in exchange for an individual using the services
of a laboratory. Although EKRA's language is similar to the language of the federal Anti-Kickback Statute, EKRA is broader than
the AKS in that it applies to all health care benefit programs, including private payors, while the AKS applies only to items and services
paid for by federal health care programs.

 Federal
and state law enforcement authorities scrutinize arrangements between laboratories and potential referral sources to ensure that the
arrangements are not designed as a mechanism to induce patient care referrals. The law enforcement authorities and the courts have also
demonstrated a willingness to look behind the formalities of a transaction to determine the underlying purpose of payments between health
care providers and actual or potential referral sources. Generally, courts have taken a broad interpretation of the scope of the federal
Anti-Kickback Statute, holding that the statute may be violated if merely one purpose of a payment arrangement is to induce referrals,
even if the arrangement has other, legitimate purposes.

 In
December 1994, the HHS Office of Inspector General, or OIG, issued a Special Fraud Alert on arrangements for the provision of clinical
laboratory services. The Fraud Alert set forth a number of practices allegedly engaged in by some clinical laboratories and health care
providers that raise issues under the federal fraud and abuse laws, including the federal Anti-Kickback Statute. The OIG emphasized in
the Special Fraud Alert that when one purpose of such arrangements is to induce referrals of program-reimbursed laboratory testing, both
the clinical laboratory and the health care provider (e.g., physician) may be liable under the federal Anti-Kickback Statute and may
be subject to criminal prosecution and exclusion from participation in the Medicare and Medicaid programs.

 Recognizing
that the federal Anti-Kickback Statute is broad and may technically prohibit many innocuous or beneficial arrangements within the health
care industry, Congress authorized, and HHS has issued, a series of regulatory "safe harbors." These safe harbor regulations
set forth certain provisions which, if all of their requirements are met, will assure health care providers and other parties that they
may not be prosecuted under the federal Anti-Kickback Statute. Although full compliance with these provisions ensures against prosecution
under the federal Anti-Kickback Statute, the failure of a transaction or arrangement to fit within a specific safe harbor does not necessarily
mean that the transaction or arrangement is illegal or that prosecution under the federal Anti-Kickback Statute will be pursued. A non-safe
harbored arrangement is evaluated by government enforcement agencies on a case-by-case basis.

 In
addition, the federal False Claims Act prohibits a person from knowingly submitting or causing to be submitted a false claim or making
a false record or statement material to a false claim in order to secure payment by the federal government. Violation of the federal
False Claims Act may result in fines of up to three times the actual damages sustained by the government, plus mandatory civil penalties
of up to $27,894 for each separate false claim.

 Moreover,
a federal law directed at "self-referrals," commonly known as the Stark Law, prohibits, with certain exceptions, laboratories
from presenting or causing to be presented claims to Medicare and Medicaid for laboratory tests referred by physicians who personally,
or through a family member, have an investment interest in, or a compensation arrangement with, the clinical laboratory performing the
tests. A person who engages in a scheme to circumvent the Stark Law's referral prohibition may be fined up to $100,000 for each
such arrangement or scheme. In addition, any person who presents or causes to be presented a claim to the Medicare or Medicaid program
in violation of the Stark Law is subject to civil monetary penalties of up to $15,000 per claim submission, an assessment of up to three
times the amount claimed, and possible exclusion from participation in federal health care programs. Claims submitted in violation of
the Stark Law may not be paid by Medicare or Medicaid, and any person collecting any amounts with respect to any such prohibited claim
is obligated to refund such amounts. Many states, including California, also have "anti-self-referral" and other laws that
are not limited to Medicare and Medicaid referrals.

 Further,
in addition to the privacy and security regulations described above, HIPAA created two federal crimes: health care fraud and false statements
relating to health care matters. The health care fraud statute prohibits knowingly and willfully executing a scheme to defraud any health
care benefit program, including both government and private payors. A violation of this statute is a felony and may result in fines,
imprisonment and/or exclusion from government sponsored programs. The false statements statute prohibits knowingly and willfully falsifying,
concealing or covering up a material fact or making any materially false, fictitious or fraudulent statement in connection with the delivery
of or payment for health care benefits, items or services. A violation of this statute is a felony and may result in fines and/or imprisonment.

 Finally,
federal law prohibits any entity from offering or transferring to a Medicare or Medicaid beneficiary any remuneration that the entity
knows or should know is likely to influence the beneficiary's selection of a particular provider, practitioner or supplier of Medicare
or Medicaid payable items or services, including waivers of copayments and deductible amounts (or any part thereof) and transfers of
items or services for free or for other than fair market value. Entities found in violation may be liable for civil monetary penalties
of up to $20,000 for each wrongful act.

 Employees

 As
of December 31, 2024, we had 66 full-time employees. We generally engage independent contractors on a part-time basis from time to time.

 Corporate
Information

 We
were originally incorporated in 1993, and we had our initial public offering in 2000. Our executive offices are located at 12117 Bee
Caves Road, Building III, Suite 100, Austin, Texas 78738, and our telephone number is (512) 519-0400. We maintain a website at www.aspirawh.com
where general information about us is available.

 Information
About Us

 We
file annual reports, quarterly reports, current reports, proxy statements, and other information with the SEC.

 The
SEC maintains an Internet website, www.sec.gov, that contains reports, proxy statements, and other information regarding issuers that
file electronically with the SEC.

 The
information contained on our websites is not incorporated by reference in this Annual Report on Form 10-K, and should not be considered
a part of this Annual Report on Form 10-K.

 ITEM
 1A.

 RISK FACTORS

 Investing
in our securities involves a high degree of risk. You should carefully consider the following risk factors and uncertainties together
with all of the other information contained in this Annual Report on Form 10-K, including our audited consolidated financial statements
and the accompanying notes in Part II Item 8, "Consolidated Financial Statements and Supplementary Data." If any of the following
risks materializes, our business, financial condition, results of operations and growth prospects could be materially adversely affected,
and the value of an investment in our common stock may decline significantly. The risks and uncertainties described below are not the
only ones we face. Additional risks and uncertainties not presently known to us or that we currently deem immaterial may also materially
adversely affect our business, financial condition, results of operations and growth prospects.

 RISKS
RELATED TO OUR BUSINESS AND INDUSTRY

 If
we are unable to increase the volume of OvaSuite sales, our business, results of operations and financial condition will be adversely
affected.

 We
have experienced significant operating losses each year since our inception, and we expect to incur a net loss for fiscal year 2025.
Our losses have resulted principally from costs incurred in cost of revenue, sales and marketing, general and administrative costs and
research and development. The number of tests performed in 2024 and in 2023 was 24,305 and 23,990, respectively. If we are unable to
substantially increase the volume of OvaSuite sales, our business, results of operations and financial condition will be adversely affected.

 There
is substantial doubt about our ability to continue as a going concern, and this may adversely affect our stock price and our ability
to raise capital.

 We
have incurred significant losses and negative cash flows from operations since inception and have an accumulated deficit of $531,397,000
as of December 31, 2024. We also expect to incur a net loss and negative cash flows from operations in 2025
and have limited cash balances. Given these conditions, there is substantial doubt about our ability to continue as a going concern.
The substantial doubt about our ability to continue as a going concern may adversely affect our stock price and our ability to raise
capital. Our independent registered public accounting firm has also included in its report an explanatory paragraph regarding this uncertainty.

 We
believe that successful achievement of our business objectives will require additional financing. We expect to raise capital through
a variety of sources that may include public or private equity offerings, debt financing, collaborations, licensing arrangements, grants
and government funding and strategic alliances. However, in part due to our low stock price, additional financing may not be available
when needed or on terms acceptable to us. If we are unable to obtain additional capital, we may not be able to continue sales and marketing,
research and development, distribution or other operations on the scope or scale of current activity and that could have a material adverse
effect on our business, results of operations and financial condition. The accompanying financial statements do not include any adjustments
that may be necessary should we be unable to continue as a going concern.

 We
may need to sell additional shares of our common stock or other securities in the future to meet our capital requirements, which could
cause significant dilution.

 Until
such time, if ever, as we can generate substantial product revenues, we expect to finance our cash needs through a combination of the
issuance of common stock in public or private equity offerings, debt financings, exercise of common stock warrants, collaborations, licensing
arrangements, grants and government funding and strategic alliances. Our management believes the successful achievement of our business
objectives will require additional financing through one or more of these avenues. To the extent that we raise additional capital through
the sale of equity or convertible debt, such financing may be dilutive to stockholders. Debt financing, if available, may involve restrictive
covenants and potential dilution to stockholders. Furthermore, a perception that future sales of our common stock in the public market
are likely to occur could affect prevailing trading prices of our common stock.

 As
of December 31, 2024, we had 17,407,120 shares of our common stock outstanding and 530,613 shares of our common stock reserved for future
issuance to employees, directors and consultants pursuant to our employee stock plans, which excludes 876,249 shares of our common stock
that were subject to outstanding options and 149,061 restricted stock units. In addition, as of December 31, 2024, warrants to purchase
4,475,068 shares of our common stock were outstanding. These warrants are exercisable at the election of the holders thereof, in accordance
with the terms of the related warrant, at an average exercise price of $2.90 per share.

 The
exercise of all or a portion of our outstanding options and warrants will dilute the ownership interests of our stockholders.

 We
will need to raise additional capital in the future, and if we are unable to secure adequate funds on terms acceptable to us, we may
be unable to execute our business plan.

 We
will seek to raise additional capital through the issuance of equity or debt securities in the public or private markets, or through
a collaborative arrangement or sale of assets. Additional financing opportunities may not be available to us, or if available, may not
be on favorable terms. The availability of financing opportunities will depend, in part, on market conditions, and the outlook for our
business. Any future issuance of equity securities or securities convertible into equity could result in substantial dilution to our
stockholders, and the securities issued in such a financing may have rights, preferences or privileges senior to those of our common
stock. If we are unable to obtain additional capital, we may not be able to continue our sales and marketing, research and development,
distribution or other operations on the scope or scale of our current activity.

 Failures
by third-party payers to reimburse for our products and services or changes in reimbursement rates could materially and adversely affect
our business, financial condition and results of operations. In addition, changes in medical society guidelines may also adversely affect
payers and result in a material change in coverage, adversely affecting our business, financial condition and results of operations.

 The
great majority of laboratory tests in the United States are paid for by third party payers. Accordingly, our current revenues are from,
and our future revenues will be dependent upon, third-party reimbursement payments to Aspira Labs. Insurance coverage and reimbursement
rates for diagnostic tests are uncertain, subject to change and particularly volatile during the early stages of commercialization. There
remain questions as to what extent third-party payers, like Medicare, Medicaid and private insurance companies will provide coverage
for our products and for which indications. Some payers have determined not to cover our tests. While Novitas Solutions, the Medicare
Administrative Contractor responsible for paying Medicare claims for all Aspira laboratory tests, has determined to cover Ova1, there
is no assurance that they will continue to do so. Moreover, while The Centers for Medicare & Medicaid Services ("CMS")
has issued PAMA reimbursement rates for Ova1 effective January 1, 2018, there is no guarantee that the payment rates will not be reduced.
Although the PAMA legislation allows for no more than a 15% fee reduction between 2025 and 2026, uncertainty regarding reimbursement
rates could create payment uncertainty from other payers as well. The reimbursement rates for Ova1 and OvaWatch are reviewed by
third-party payers. We have experienced volatility in the coverage and reimbursement of our products due to contract negotiation with
third-party payers and implementation requirements, and the reimbursement amounts we have received from third-party payers varies from
payer to payer, and, in some cases, the variance could be material.

 Third-party
payers, including private insurance companies as well as government payers such as Medicare and Medicaid, have increased their efforts
to control the cost, utilization and delivery of healthcare services including increased use of Laboratory Benefits Management firms,
who create policy and implement utilization management strategies for their payer clients to ensure tests are medically necessary. In
addition, more payers are implementing pre-authorization requirements for our testing. These measures have resulted in reduced payment
rates and decreased utilization of our tests. Further, the trend among many payers is to limit the size of their lab networks, which
is making it more difficult to secure preferred provider contracts for some services. From time to time, Congress has considered and
implemented changes to the Medicare fee schedules in conjunction with budgetary legislation, and pricing for tests covered by Medicare
is subject to change at any time, although PAMA has established specific dates by which they will make any changes. Even if favorable
coverage and reimbursement status is attained for one or more products by governmental and commercial third-party payers, less favorable
coverage policies and reimbursement rates may be implemented in the future. Reductions in third-party payer reimbursement rates may occur
in the future. Reductions in the price at which our products are reimbursed could have a material adverse effect on our business, results
of operations and financial condition. If we are unable to establish and maintain broad coverage and adequate reimbursement for our products
or if third-party payers change their coverage or reimbursement policies with respect to our products, our business, financial condition
and results of operations could be materially adversely affected.

to continue coverage of Ova1 through Novitas, our Medicare Administrative Contractor for Ova1, could materially and adversely affect
our business, financial condition and results of operation.

 Since
2013, Ova1 has been listed as a covered service in the Biomarkers for Oncology Local Coverage Determination (the "Biomarkers for
Oncology LCD") issued by Novitas, the Medicare Administrative Contractor responsible for payment of Medicare claims for all Aspira
Labs tests. In June 2023, in conjunction with the publication of a final "Genetic Testing for Oncology" LCD (the "Genetic
Testing LCD"), Novitas announced that it intended to retire the Biomarkers for Oncology LCD effective July 17, 2023, and that at
that time, non-genetic tests currently identified as covered in that LCDs (like Ova1) would be considered for payment based on Medicare
medically reasonable and necessary threshold for coverage.

 On
July 6, 2023, Novitas issued a statement announcing that the Genetic Testing LCD would not go into effect on July 17, 2023 as planned,
and that a new proposed LCD would be published for public comment. Novitas issued a replacement proposed LCD for public comment on July
27, 2023. The Biomarkers for Oncology LCD remains in effect.

 All
OvaSuite tests (Ova1, Overa, Ova1Plus and OvaWatch) are protein-based multivariate index assays and were not impacted by the now-withdrawn
Genetic Testing LCD. While we do not believe Novitas intends to eliminate Ova1 coverage, it is impossible to assess the likelihood or
potential impact, if any, of future actions to be taken by Novitas with respect to the release of a replacement Genetic s Testing
LCD, or a change to the content or status of the Biomarkers for Oncology LCD, on the coverage and related revenue of Ova1, and such impact
may be material to our business, results of operations and financial condition. We are monitoring developments closely and believe additional
due process would be required if the activities contemplated by Novitas change the coverage determination for Ova1.

to expand commercial, Medicare or Medicaid coverage for our products could materially and adversely affect our business, financial condition
and results of operations.

 We
have implemented strategies to expand payer coverage for our ovarian cancer risk assessments, including securing coverage for OvaWatch
that is consistent with existing coverage for Ova1. In November 2023, CMS approved our request to provide reimbursement for OvaWatch
that is consistent with the reimbursement for Ova1 at $897 per test. However, there can be no assurances that we will be able to secure
additional payer coverage for Ova1 and comparable coverage for OvaWatch, or that that the reimbursement rate for OvaWatch will not be
reduced. Failure to expand payer coverage and maintain adequate reimbursement rates may have a significant negative impact on product
adoption and our results of operations.

 We
may not succeed in improving existing or developing additional diagnostic products, and, even if we do succeed in developing additional
diagnostic products, the diagnostic products may never achieve significant commercial market acceptance.

 Our
technologies are new and complex and are subject to change as new discoveries are made. New discoveries and advancements in the diagnostic
field are essential if we are to foster the adoption of our product offerings. Development of our existing technologies remains a substantial
risk to us due to various factors, including the scientific challenges involved within our laboratory, as well as products that are offered
in a decentralized platform such as Aspira Synergy, our ability to find and collaborate successfully with others working in the diagnostic
field, our ability to obtain sufficient samples to complete the design and development of our algorithms and competing technologies,
which may prove more successful than our technologies, as well as failure to complete analytical and clinical validation studies and
failure to demonstrate sufficient clinical utility to continue to build positive medical policy among payers.

 Our
failure to achieve the intended development outcome either ourselves or through a collaboration may result in an impact to our commercial
success of our risk assessment screens for endometriosis or other product launches.

 Our
success depends on our ability to continue to develop and commercialize diagnostic products. There is considerable risk in developing
diagnostic products based on our biomarker discovery efforts, as candidate biomarkers may fail to demonstrate clinical validity in larger
clinical studies or may not achieve acceptable levels of analytical accuracy. For example, markers being evaluated for one or more next-generation
diagnostic tests may not be validated in downstream pre-clinical or clinical studies, once we undertake and perform such studies. In
addition, development of products combining biomarkers with imaging, patient risk factors or other risk indicators carry higher than
average risks due to technical, clinical and regulatory uncertainties. While we have a published proof of concept on combining Ova1 and
imaging, for example, our ability to develop, verify and validate an algorithm that generalizes to routine testing populations cannot
be guaranteed. In addition, our efforts to develop other diagnostic tests, such as ENDOinform and OVAinform, are in the early development
phase, and future pre-clinical or clinical studies may not support our early data. If successful, the regulatory pathway and clearance/approval
process may require extensive discussion with applicable authorities and possibly advisory panels. These pose considerable risk in projecting
launch dates, requirements for clinical evidence and eventual pricing and return on investment. Although we are engaging important stakeholders
representing gynecologic oncology, benign gynecology, patient advocacy, women's health research, legislators, payers, and others,
success, timelines and value will be uncertain and require active management at all stages of innovation and development.

testing is expensive, can take many years to complete and can have an uncertain outcome. Clinical failure can occur at any stage of the
testing. Clinical trials for our next generation ovarian cancer tests, and other future diagnostic tests, may produce negative or inconclusive
results, and we may decide, or regulators may require us, to conduct additional clinical and/or non-clinical testing on these tests.
In addition, the results of our clinical trials may identify unexpected risks relative to safety or efficacy, which could complicate,
delay or halt clinical trials, or result in the denial of regulatory approval by the FDA and other regulatory authorities.

 If
we do succeed in developing additional diagnostic tests with acceptable performance characteristics, we may not succeed in achieving
commercial market acceptance for those tests. Our ability to successfully commercialize our OvaSuite products and Aspira Synergy platform
will depend on many factors, including:

 our
 ability to drive adoption of our products;

 our
 success in establishing new clinical practices or changing previous ones;

 our
 ability to develop business relationships with diagnostic or laboratory companies that can assist in the commercialization of these
 products in the U.S. and globally; and

 the
 scope and extent of the agreement by Medicare and third-party payers to provide full or partial reimbursement coverage for our products,
 which may impact patients' willingness to pay for our products and may influence physicians' decisions to recommend or
 use our products.

 These
factors present obstacles to commercial acceptance of our existing and potential diagnostic products, for which we will have to spend
substantial time and financial resources to overcome, and there is no guarantee that we will be successful in doing so. Our inability
to do so successfully would prevent us from generating revenue from OvaSuite and developing future diagnostic products.

 In
October 2022, we announced the launch of a comarketing arrangement for the Ova1Plus workflow with BioReference. Under terms of the agreement,
the Aspira and BioReference sales teams collaborate to sell Ova1Plus to gynecologists and other women's healthcare providers nationwide.
In November 2024, Aspira and BioReference announced the expansion of the sale team collaboration to include OvaWatch. If we are unable
to collaborate successfully, it may affect our ability to improve adoption of our Ova1Plus test or to successfully secure additional
commercial collaborations.

 The
diagnostics market is competitive, and we may not be able to compete successfully, which would adversely impact our ability to generate
revenue.

 Our
principal competition currently comes from the many clinical options available to medical personnel involved in clinical decision making.
For example, rather than ordering an OvaSuite test for a woman with an adnexal mass, obstetricians, gynecologists and gynecologic oncologists
may choose a different clinical option or none at all. If we are not able to convince clinicians that these products provide significant
improvement over current clinical practices or to change their ordering habits, our ability to commercialize OvaSuite products will be
adversely affected.

 Competitive
offerings include Fujirebio Diagnostics' FDA cleared ROMA test. ROMA combines two tumor markers and menopausal status into a numerical
score using a publicly available algorithm. ROMA is a competitive test with the Ova1Plus workflow that has adversely impacted and may
continue to materially adversely impact our revenue. In addition, competitors, AOA Dx, ClearNote, Cleo Diagnostics, Mercy BioAnalytics,
and others have publicly disclosed that they have been or are currently working on ovarian cancer diagnostic assays. Exact Sciences,
Grail, and others are working on multi-cancer early diagnostic tests that include ovarian cancer detection. Academic institutions periodically
report new findings in ovarian cancer diagnostics that may have commercial value.

 A
number of diagnostic and academic organizations have announced plans or published studies related to the development of a non-invasive
diagnostic tool for the identification of endometriosis. Competitors for our endometriosis offerings include, but are not limited to,
Afynia, DotLab, Endodiag, HERA Biotech, Heranova, Proteomics International. and Ziwig. Our failure to compete with any competitive diagnostic
assay if and when commercialized could adversely affect our business, financial condition and results of operations.

 We
have priced our products at a point that recognizes the value-added by its increased sensitivity for detecting ovarian malignancy. If
others develop a test that is viewed to be similar to any of these products in safety and efficacy but is priced at a lower point, we
and/or our strategic partners may have to lower the price of that product in order to effectively compete, which would impact our margins
and potential for profitability.

 We
are currently offering and developing multiple tests as LDTs and intend to develop and perform LDTs at Aspira Labs in the future. FDA's
newly-issued rule for LDTs, which will be phased in over a period of four years, will significantly change the regulatory landscape for
LDTs. Unless the rule is overturned by a court or superseded by Congressional action, our currently marketed LDTs and those we develop
in the future will be subject to new requirements including, for some tests, premarket authorization. The new rule will lead to additional
compliance costs and may delay or prevent market entry for new or modified tests and there is a risk that their commercialization, and
our results of operations and financial condition, will be negatively affected.

 The
FDA considers an LDT to be a test that is designed, developed, validated, and used within a single, CLIA-certified high complexity laboratory.
The FDA has historically taken the position that it has the authority to regulate LDTs as in vitro diagnostic ("IVD") medical
devices under the FDC Act, but it has generally exercised enforcement discretion with regard to LDTs, meaning that most LDTs have not
been subject to FDA oversight. On May 6, 2024, the FDA published a final rule amending the definition of an IVD device to include IVDs
manufactured by a clinical laboratory, effectively codifying its position that LDTs are IVDs and, therefore, that LDTs fall under FDA's
regulatory authority. The final rule also announced the FDA's intention to phase out its general enforcement discretion policy.
Unless the rule is overturned by a court or superseded by Congressional action, the medical device requirements for most LDTs will be
phased in beginning on May 6, 2025.

 The
new rule implements a phased approach to ending FDA's policy of enforcement discretion for LDTs. The phased approach establishes
timelines for LDT sponsors to comply with different categories of FDA device regulations, and the clock starts on the final rule's
publication date – May 6, 2024, the date to which all five phases are anchored. The phases are as follows: (1) LDTs are subject
to Medical Device Reporting ("MDR"), as well as adverse event reporting, one year after the final rule's publication
date (i.e., May 6, 2025); (2) LDTs are subject to registration/listing, labeling, and investigational use requirements two years after
the final rule's publication date (i.e., May 6, 2026); (3) LDTs are subject to Quality System regulations three years after the
final rule's publication date (i.e., May 6, 2027); (4) high-risk LDTs are subject to premarket review (i.e., 510(k) clearance,
 de novo classification, or PMA, as applicable) three-and-a-half years after the final rule's publication date (i.e., Nov.
6, 2027), unless a premarket submission has been received by the beginning of this stage in which case FDA intends to continue to exercise
enforcement discretion for the pendency of its review; and (5) mid- and low-risk LDTs are subject to premarket review (i.e., 510(k) clearance,
 de novo classification, or PMA, as applicable) four years after the final rule's publication date (i.e., May 6, 2028), unless
a premarket submission has been received by the beginning of this stage in which case FDA intends to continue to exercise enforcement
discretion for the pendency of its review.

categories of LDTs will be subject to enforcement discretion with respect to some or all of these requirements. In total, the new rule
identifies eight (8) types of LDTs for which it will continue to exercise enforcement discretion with respect to some or all regulatory
requirements. For example, the FDA will apply enforcement discretion to currently marketed LDTs that were first offered prior to May
6, 2024, with respect to most quality system requirements and the requirement for premarket authorization if they are not modified or
modified in only limited ways. The FDA will similarly exercise enforcement discretion with respect to premarket authorization for LDTs
approved by the New York State Clinical Laboratory Evaluation Program. However, laboratories performing these tests are subject to all
other requirements outlined in FDA's phase-out policy, including, but not limited to, the requirement to submit the labeling for
the LDT to FDA for review. As outlined in the new rule, FDA will also exercise enforcement discretion with respect to some or all regulatory
requirements for certain LDTs designed for rare, unmet, or specific needs, LDTs manufactured and performed within the Veterans Health
Administration ("VHA") or the Department of Defense ("DoD"), so-called "1967-Type LDTs," and forensic
use LDTs.

 Compliance
with these additional regulatory requirements will be time-consuming and expensive, potentially diverting resources from other aspects
of our business, and will potentially affect the sales of our products and how customers use our products and will require reevaluation
of our business model in order to maintain compliance with these laws. Moreover, failure to comply with these and other FDA regulations
could result in legal actions, including fines, penalties, and exclusion from federal healthcare programs (e.g., Medicare).

 If
we are unable to comply with FDA requirements, or to do so within the timeframes specified by the FDA, we may be forced to stop selling
our tests or be required to modify claims or make such other changes while we update our processes. For existing or future tests subject
to FDA clearance, approval or de novo classification, our business, results of operations and financial condition will be negatively
affected until such a review is completed and clearance, approval or de novo classification to market were obtained. There can
be no assurance that any tests we develop will be cleared, approved or classified on a timely basis, if at all. Obtaining FDA clearance,
approval or de novo classification for diagnostics can be expensive, time consuming and uncertain, and for higher-risk devices
generally takes several years and requires detailed and comprehensive scientific and clinical data. Ongoing compliance with FDA regulations
for those tests will increase the cost of conducting our business, significantly affect our operations, and could have a significant
negative impact on our financial performance.

 Legislative
proposals addressing the FDA's oversight of LDTs have been previously introduced. In June 2021, Congress introduced the Verifying
Accurate, Leading-edge IVCT Development ("VALID") Act, which would have established a new risk-based regulatory framework
for in vitro clinical tests ("IVCTs"), a category which would have included IVDs, LDTs, collection devices and instruments
used with such tests. This legislation was not enacted during that session of Congress, but was reintroduced in 2023. FDA's new
LDT final rule may renew attention to VALID and may lead to the introduction of new proposals to limit the FDA's regulatory authority.

 FDA's
new rule, which establishes the phase-out policy for enforcement discretion with respect to LDTs, has been challenged in two separate
lawsuits – one brought in the District Court for the Eastern District of Texas and the other brought in the District Court for
the Southern District of Texas. As of the time of this filing, both cases are still pending.

 Our
diagnostic tests and software are subject to ongoing regulation by the FDA, and any delay by or failure of the FDA to authorize our diagnostic
tests submitted to the FDA may adversely affect our business, results of operations and financial condition.

 Our
activities related to diagnostic products are, or have the potential to be, subject to regulatory oversight by the FDA under provisions
of the FDC Act and regulations thereunder, including regulations governing the development, marketing, labeling, promotion, manufacturing
and export of our products. Failure to comply with applicable requirements can lead to sanctions, including withdrawal of products from
the market, recalls, refusal to authorize government contracts, product seizures, civil money penalties, injunctions and criminal prosecution.

 The
FDC Act requires that medical devices introduced to the United States market, unless exempted by regulation, be authorized by FDA pursuant
to either the premarket notification pathway, known as 510(k) clearance, the de novo classification pathway, or the PMA pathway.
The FDA granted a request for a de novo authorization for Ova1 in September 2009, and we commercially launched Ova1 in March 2010.
In March 2016, we received FDA 510(k) clearance for a second-generation biomarker panel known as Ova1 Next Generation, which we call
Overa. Ova1 was the first FDA-cleared blood test for the pre-operative assessment of ovarian masses. With respect to devices reviewed
through the 510(k) process, we may not market a device until it is determined that our product is substantially equivalent to a legally
marketed device known as a predicate device. A 510(k) submission may involve the presentation of a substantial volume of data, including
clinical and analytical data, as well as extensive information regarding software. The FDA may agree that the product is substantially
equivalent to a predicate device and allow the product to be marketed in the United States. On the other hand, the FDA may determine
that the device is not substantially equivalent and require a PMA or de novo classification, or require further information, such
as additional test data, including data from clinical studies, before it is able to make a determination regarding substantial equivalence.
By requesting additional information, the FDA can delay market introduction of our products. Delays in receipt of or failure to receive
any necessary 510(k) clearance, de novo classification, or PMA, or the imposition of stringent restrictions on the labeling and
sales of our products, could have a material adverse effect on our business, results of operations and financial condition. If the FDA
determines that a PMA is required for any of our potential future clinical products, the application will require extensive clinical
studies, manufacturing information and could require review by an FDA advisory panel comprising experts outside the FDA. Clinical studies
to support a 510(k) submission, de novo classification or a PMA application would need to be conducted in accordance with FDA
requirements. Failure to comply with FDA requirements could result in the FDA's refusal to accept the submission or denial of the
application. We cannot ensure that any necessary 510(k) clearance, de novo classification, or PMA will be granted on a timely
basis, or at all. To the extent we seek FDA 510(k) clearance, de novo classification or FDA pre-market approval for other diagnostic
tests, any delay by or failure of the FDA to clear, classify, or approve those diagnostic tests may adversely affect our consolidated
revenues, results of operations and financial condition.

changes to medical devices that a manufacturer makes after receiving a 510(k) clearance, de novo classification, or PMA may trigger
the need for additional FDA authorization. In the case of a 510(k)-cleared device, FDA requires a new marketing authorization for significant
changes or modifications made in the design, components, method of manufacture or intended use of a device, including changes or modifications
to a 510(k)-cleared device that could significantly affect the device's safety or effectiveness, or would constitute a major change
or modification in the device's intended use. The type of submission needed—510(k), de novo classification, or PMA—will
depend on the specific modification the manufacturer seeks to make. FDA expects the manufacturer to make the determination of whether
a new marketing application is needed by applying existing agency guidance, but FDA may independently review, and may disagree with,
our decision. If we make modifications to our marketed devices, we may be required to seek additional clearances, de novo classifications,
or PMAs which, if not granted, would prevent us from selling the modified device. If we conclude that a modification does not require
submission of a new marketing application and FDA disagrees with the decision, we may be required to submit new 510(k) notifications,
 de novo classification requests, or premarket approval applications and may be required to cease marketing of or to recall the
modified devices until marketing authorization is obtained and could additionally be subject to regulatory fines or penalties. Any recall
or FDA requirement that we seek additional approvals or clearances could result in significant delays, fines, increased costs associated
with modification of a product, loss of revenue and potential operating restrictions imposed by the FDA.

of our software algorithms have been authorized for marketing by FDA as part of our cleared or de novo classified tests. If any
of the software that we use in our LDTs or that we make available to third parties is determined by FDA to be non-exempt clinical decision
support software, this could impede our ability to offer our tests or distribute our software to third parties and we could incur substantial
costs and delays associated with trying to obtain premarket 510(k) clearance, de novo classification, or premarket review and
incur costs associated with complying with post-market controls.

 If
we or our suppliers fail to comply with FDA requirements for production, marketing and post-market monitoring of our products, we may
not be able to market our products and services and may be subject to stringent penalties, product restrictions or recall.

to comply with FDA requirements for post-market monitoring of our products may affect the commercialization of our products, therefore
adversely affecting our business. The FDA granted the request for de novo classification for Ova1 in September 2009 and cleared
Overa in March 2016. Post-market surveillance studies were conducted to further analyze performance of Ova1 and Overa. These studies
have been completed and closed with the FDA.

 Additionally,
if the FDA were to view any of our actions as non-compliant, it could initiate enforcement actions, such as a warning letter and possible
imposition of penalties. For instance, we are subject to a number of FDA requirements, including compliance with the FDA's QSR
requirements, which establish extensive requirements for quality assurance and control as well as manufacturing procedures. Failure to
comply with these regulations could result in enforcement actions for us or our potential suppliers. Adverse FDA actions in any of these
areas could significantly increase our expenses and reduce our revenue. We will need to undertake steps to maintain our operations in
line with the FDA's QSR requirements. Some components of Ova1 and Overa are manufactured by other companies and we are required
to ensure that, to the extent that we incorporate those components into our finished Ova1 and Overa products, we use those components
in compliance with QSR. Any failure to do so would have an adverse effect on our ability to commercialize the Ova1Plus workflow. Our
suppliers that manufacture finished devices at their manufacturing facilities that we use in our products and services are subject to
periodic regulatory inspections by the FDA and other federal and state regulatory agencies. Our facility also is subject to FDA inspection.
We or our suppliers may not satisfy such regulatory requirements, and any such failure to do so may adversely affect our business, financial
condition and results of operations.

 If
our suppliers fail to produce acceptable or sufficient stock, fail to supply stock due to supply shortages, make changes to the design
or labeling of their biomarker kits or discontinue production of existing biomarker kits or instrument platforms, we may be unable to
meet market demand for OvaSuite products.

 The
commercialization of our OvaSuite tests depends on the supply of seven different immunoassay kits from third-party manufacturers that
run on automated instruments. Failure by any of these manufacturers to produce kits that meet our specifications and pass our quality
control measures might lead to back-order and/or loss of revenue due to missed sales and customer dissatisfaction. In addition, if the
design or labeling of any kit were to change, continued OvaSuite supply could be threatened since new validation and submission to the
FDA for review could be required as a condition of sale. Discontinuation of any of these kits could require identification, validation
and submission to FDA of a revised OvaSuite design. Likewise, discontinuation or failure to support or service the instruments may pose
risk to ongoing operations.

 Changes
in healthcare policy could increase our costs and adversely impact sales of and reimbursement for our tests, which would have an adverse
effect on our business, financial condition and results of operations.

 PAMA
established a Medicare reimbursement system for clinical laboratories beginning in 2018 that is based on rates paid to laboratories by
private payers. The CMS also issued various regulations and guidance to implement PAMA that require certain laboratories to report information
on the rates private payers pay them for laboratory tests, including Multianalyte Assays with Algorithmic Analyses. In addition to these
changes, a number of states are also contemplating significant reform of their healthcare reimbursement policies. We expect that there
will be additional health reform initiatives by legislators at both the federal and state levels, regulators and third-party payers to
reduce costs while expanding individual healthcare benefits. Certain of these changes could impose additional limitations on the rates
we will be able to charge for our current and future products or the amounts of reimbursement available for our current and future products
from governmental agencies or other third-party payers. We cannot predict whether future healthcare initiatives will be implemented at
the federal or state level, or the effect any future legislation or regulation will have on us. Other changes to healthcare laws may
adversely affect our business, financial condition and results of operations.

 We
are subject to environmental laws and potential exposure to environmental liabilities.

 We
are subject to various international, federal, state and local environmental laws and regulations that govern our operations, including
the handling and disposal of non-hazardous and hazardous wastes, the recycling and treatment of electrical and electronic equipment,
and emissions and discharges into the environment. Failure to comply with such laws and regulations could result in costs for corrective
action, penalties or the imposition of other liabilities. We are also subject to laws and regulations that impose liability and clean-up
responsibility for releases of hazardous substances into the environment. Under certain of these laws and regulations, a current or previous
owner or operator of property may be liable for the costs to remediate hazardous substances or petroleum products on or from its property,
without regard to whether the owner or operator knew of, or caused, the contamination, as well as incur liability to third parties affected
by such contamination. The presence of, or failure to remediate properly, such substances could adversely affect the value and the ability
to transfer or encumber such property.

 The
operation of Aspira Labs requires us to comply with numerous laws and regulations, which is expensive and time-consuming and could adversely
affect our business, financial condition and results of operations, and any failure to comply could result in exposure to substantial
penalties and other harm to our business.

 In
June 2014, we launched a clinical laboratory, Aspira Labs, in Texas. Clinical laboratories that perform tests on human subjects in the
United States for the purpose of providing information for the diagnosis, prevention or treatment of disease or the assessment of human
health must be certified under CLIA and licensed or permitted under applicable state laboratory laws. CLIA is a federal law that regulates
the quality of clinical laboratory testing by requiring laboratories to comply with various technical, operational, personnel and quality
requirements intended to ensure that the services provided are accurate, reliable and timely. A few states, including New York State,
may require that additional quality standards be met and that detailed review of scientific validations and technical procedures for
tests occur. In the future, the federal government may change the way that clinical laboratory tests are regulated, which may adversely
affect our business, financial condition and results of operations.

Labs holds a CLIA Certificate of Accreditation and a state laboratory license or permit in California,
 Maryland, New York, Pennsylvania and Rhode Island. This allows the lab to perform
Ova1 and Overa testing (through the Ova1Plus workflow) on a national basis. We are subject to periodic surveys and inspections to maintain
our CLIA certification, and such certification is also required to obtain payment from Medicare, Medicaid and certain other third-party
payers. Failure to comply with CLIA or state law requirements may result in the imposition of corrective action or the suspension or
revocation of our CLIA certification or state licenses. If our CLIA certification or state licenses are suspended or revoked or our right
to bill the Medicare and Medicaid programs or other third-party payers is suspended, we would no longer be able to sell our tests, which
would adversely affect our business, financial condition and results of operations.

 In
addition, no assurance can be given that Aspira Labs' suppliers or commercial partners will remain in compliance with applicable
CLIA and other federal or state regulatory requirements for laboratory operations and testing. Aspira Labs' facilities and procedures
and those of Aspira Labs' suppliers and commercial partners are subject to ongoing regulation, including periodic inspection by
regulatory and other government authorities. The principal sanction under CLIA is suspension, limitation or revocation of a lab's
CLIA certificate. CMS also may impose the following alternative sanctions: (a) directed plan of correction, (b) state onsite monitoring,
and/or (c) civil monetary penalty. In addition, the government may bring suit to enjoin any activity of any laboratory that has been
found with deficiencies during a survey if CMS has reason to believe that continuation of the activity would constitute a significant
hazard to the public health. Finally, criminal sanctions may be imposed on an individual who is convicted of intentionally violating
any CLIA requirement.

 Our
clinical laboratory business is also subject to regulation at both the federal and state level in the United States, as well as regulation
in other jurisdictions outside of the United States, including:

 Medicare
 and Medicaid coverage, coding and payment regulations applicable to clinical laboratories;

 The
 Federal Anti-Kickback Statute, the Eliminating Kickbacks in Recovery Act and state anti-kickback prohibitions;

 the
 federal physician self-referral prohibition, commonly known as the Stark Law, and state self-referral prohibitions;

 the
 Medicare civil monetary penalty and exclusion penalty;

 the
 Federal False Claims Act civil and criminal penalties and state equivalents;

 the
 federal fraud, waste and abuse laws and state equivalents;

 the
 federal Physician Payments Sunshine Act; and

 the
 Federal Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), as amended by the Health Information Technology
 for Economic and Clinical Health Act of 2009 ("HITECH").

 Many
of these laws and regulations prohibit a laboratory from making payments or furnishing other benefits to influence the referral of tests
(by physicians or others) that are billed to Medicare, Medicaid or certain other federal or state healthcare programs. The penalties
for violation of these laws and regulations may include monetary fines, criminal and civil penalties and/or suspension or exclusion from
participation in Medicare, Medicaid and other federal healthcare programs. Several states have similar laws that may apply even in the
absence of government payers. HIPAA and HITECH and similar state laws seek to protect the privacy and security of individually identifiable
health information, and penalties for violations of these laws may include required reporting of breaches, monetary fines and criminal
or civil penalties.

 In
2020, Congress passed the Consolidated Appropriations Act and included a section called the "No Surprises Act." The No Surprises
Act prohibits a health care provider from billing a commercially insured patient more than in-network cost-sharing amounts when a service
originated from an in-network hospital or ambulatory surgery center, even if the provider is out-of-network with the patient's
health plan. It also requires a provider to provide a good faith estimate of expected charges to an uninsured or self-pay patient upon
the patient's request or when a patient schedules a service. Several states have similar laws that aim to protect patients from
unexpected health care charges. Civil penalties of up to $10,000 per occurrence can be imposed for knowing violations of the No Surprises
Act that are not remediated within a certain timeframe, and states may impose their own penalties for violations of their surprise billing
laws.

 While
we seek to conduct our business in compliance with all applicable laws and develop compliance policies to address risk as appropriate,
many of the laws and regulations applicable to us are vague or indefinite and have not been interpreted by governmental authorities or
the courts. These laws or regulations also could in the future be interpreted or applied by governmental authorities or the courts in
a manner that could require us to change our operations.

 Any
action brought against us for violation of these or other laws or regulations (including actions brought by private qui tam "whistleblower"
plaintiffs), even if successfully defended, could divert management's attention from our business, damage our reputation, limit
our ability to provide services, decrease demand for our services and cause us to incur significant expenses for legal fees and damages.
If we fail to comply with applicable laws and regulations, we could suffer significant civil, criminal and administrative penalties,
fines, recoupment of funds received by us, exclusion from participation in federal or state healthcare programs, and the loss of various
licenses, accreditations, certificates and authorizations necessary to operate our business, contractual damages, reputational harm,
diminished profits and future earnings, additional reporting obligations and oversight if we become subject to a corporate integrity
agreement or other agreement. We also could potentially incur additional liabilities from third-party claims. If any of the foregoing
were to occur, it could have a material adverse effect on our business, financial condition and results of operations.

 Our
ability to utilize our net operating loss carryforwards and certain other tax attributes may be limited.

 We
have significant net operating loss ("NOL") carryforwards as of December 31, 2024 which are subject to a full valuation allowance
due to our history of operating losses. Section 382 of the Internal Revenue Code of 1986, as amended ("Section 382"), as
well as similar state provisions restrict our ability to use our NOL carryforwards to offset taxable income due to ownership change limitations
that have occurred in the past or that could occur in the future. These ownership changes also may limit the amount of tax credit carryforwards
that can be utilized annually to offset future tax liabilities.

 Our
 pre- 2018 federal NOLs will expire in varying amounts from 2025 through 2037, if not utilized,
and can offset 100% of future taxable income for regular tax purposes. Any federal NOLs arising on or after January 1, 2018, can be carried
forward indefinitely but such federal NOL carryforwards are permitted to be used in any taxable year to offset only up to 80% of taxable
income in such year. Portions of our state NOLs will expire in varying amounts from 2025 through 2044 if not utilized. Our ability to
use our NOLs during this period will be dependent on our ability to generate taxable income, and portions of our NOLs could expire before
we generate sufficient taxable income.

 We
believe we have experienced ownership changes in the past for purposes of these limitations, and we estimate that a substantial portion
of our existing federal NOL and tax credit carryforwards are subject to annual limitation. Additional issuances or sales of our common
stock, and certain other transactions involving our stock that are outside of our control, could cause additional ownership changes.
Any current or future limitations on the use of our NOLs or tax credit carryforwards could, depending on the extent of such limitation,
result in our retaining less cash during any year in which we have taxable income than we would be entitled to retain if such limitations
did not apply, which could adversely impact our results of operations and financial condition.

 If we are unable to complete the required milestones
under our federal award milestone-based funding agreement, our business, results of operations and financial condition will be adversely
affected.

 On October 23, 2024, we announced
that we had been selected by the federal government as an awardee of a milestone-based funding agreement. A failure to meet the milestone
deadlines in the agreement would require good faith negotiations with the awarding party, including a request for an extension. There
is no guarantee that such an extension would be granted.

 Our
milestone-based funding from a federal award could be delayed or eliminated based on actions from the Trump Administration.

 On
January 27, 2025, the Trump Administration announced that all federal grants and loans would be paused for a period of time. The announcement
resulted in confusion as to what programs would be affected and for how long. As of the date of this filing, we are not aware of any
pause or termination of our federal award. However, the possibility exists that the federal award could be restricted or terminated by
the Trump Administration in the future. If the federal award is restricted or terminated, it would have a material adverse effect on
our development of our endometriosis diagnostic test which could have a material adverse effect on our financial condition, business
and results of operations.

 RISKS
RELATED TO INTELLECTUAL PROPERTY AND PRODUCT LIABILITY

 If
we fail to maintain our rights to utilize intellectual property directed to diagnostic biomarkers, we may not be able to offer diagnostic
tests using those biomarkers.

 One
aspect of our business plan is to develop diagnostic tests based on certain biomarkers, which we have the right to utilize through licenses
with our academic collaborators, such as the Johns Hopkins University School of Medicine, the University of Texas M.D. Anderson Cancer
Center, Harvard's Dana-Farber Cancer Institute, Brigham & Women's Hospital, and Medical University of Lodz. In some cases,
our collaborators own the entire right to the biomarkers. In other cases, we co-own the biomarkers with our collaborators. If, for any
reason, we lose our license to biomarkers owned entirely by our collaborators, we may not be able to use those biomarkers in diagnostic
tests. If we lose our exclusive license to biomarkers co-owned by us and our collaborators, our collaborators may license their share
of the intellectual property to a third party that may compete with us in offering diagnostic tests, which would materially adversely
affect our business, results of operations and financial condition.

 If
a third party infringes on our proprietary rights, we may lose any competitive advantage we have as a result of diversion of our time,
enforcement costs and the loss of the exclusivity of our proprietary rights.

 Our
success depends in part on our ability to maintain and enforce our proprietary rights. We rely on a combination of patents, trademarks,
copyrights and trade secrets to protect our technology and brand. We have submitted a number of patent applications covering biomarkers
that may have diagnostic or therapeutic utility. Our patent applications may or may not result in additional patents being issued.

 If
third parties engage in activities that infringe on our proprietary rights, we may incur significant costs in asserting our rights, and
the attention of our management may be diverted from our business. We may not be successful in asserting our proprietary patient rights,
which could result in our patents being held invalid or a court holding that the competitor is not infringing, either of which may harm
our competitive position. We cannot be sure that competitors will not design around our patented technology. We also may not be successful
in asserting our proprietary trademark rights, which could result in significant rebranding costs, not being able to obtain a federal
trademark registration, or a court holding that the competitor is not infringing, any of which may harm our competitive position. We
cannot be sure that competitors will not use a similar mark.

 We
also rely upon the skills, knowledge and experience of our technical personnel. To help protect our rights, we require all employees
and consultants to enter into confidentiality agreements that prohibit the disclosure of confidential information. These agreements may
not provide adequate protection for our trade secrets, knowledge or other proprietary information in the event of any unauthorized use
or disclosure. If any trade secret, knowledge or other technology not protected by a patent were to be disclosed to or independently
developed by a competitor, it could have a material adverse effect on our business, consolidated results of operations and financial
condition.

 If
others successfully assert their proprietary rights against us, we may be precluded from making and selling our products or we may be
required to obtain licenses to use their technology.

 Our
success depends on avoiding infringing on the proprietary technologies of others. If a third party were to assert claims that we are
violating its patents, we might incur substantial costs defending ourselves in lawsuits against charges of patent infringement or other
allegations of unlawful use of another's proprietary technology. Any such lawsuit may involve considerable management and financial
resources and may not be decided in our favor. If we are found liable, we may be subject to monetary damages or an injunction prohibiting
us from using the technology. We may also be required to obtain licenses under patents owned by third parties and such licenses may not
be available to us on commercially reasonable terms, if at all.

 If
a third party were to assert claims that we are violating its trademarks, we might incur substantial costs defending ourselves in lawsuits
against charges of trademark infringement. Any such lawsuit may involve considerable management and financial resources and may not be
decided in our favor. If we are found liable, we may be subject to monetary damages or an injunction prohibiting us from using the mark.
We may also be required to rebrand or enter into a co-existence agreement with a third party, which may be commercially restrictive or
unreasonable.

 Our
diagnostic efforts may cause us to have significant product liability exposure.

 The
testing, manufacturing and marketing of medical diagnostic tests entail an inherent risk of product liability claims. Potential product
liability claims may exceed the amount of our insurance coverage or may be excluded from coverage under the terms of the policy. We will
need to increase our amount of insurance coverage in the future if we are successful at introducing new diagnostic products, and this
will increase our costs. If we are held liable for a claim or for damages exceeding the limit of our insurance coverage, we may be required
to make substantial payments. This may have an adverse effect on our business, financial condition and results of operations.

of our patent registrations will expire, which may cause us to have significant competition.

 Our
success depends in part on our ability to own and assert our patent registrations to maintain and enforce our proprietary rights, including
defending against infringement actions. We have some patent registrations covering biomarkers that may be expiring, and our strategy
to continue to seek protection and file patent applications may or may not result in additional patents being issued.

 If
any such patent registration is no longer protectable and could be exploited by a competitor, it could have a material adverse effect
on our business, consolidated results of operations and financial condition.

 OPERATIONAL
RISKS

 Because
our business is highly dependent on key executives and employees, our inability to recruit and retain these people could hinder our business
plans.

 We
are highly dependent on our executive officers and certain key employees. Our executive officers and key employees are employed at will
by us. Any inability to engage new executive officers or key employees could impact operations or delay or curtail our research, development
and commercialization objectives. To continue our research and product development efforts, we need people skilled in areas such as clinical
operations, regulatory affairs and clinical diagnostics. Competition for qualified employees is intense. To continue our commercialization
objectives and reach our financial and operational goals, we require skilled sales individuals with familiarity in our industry. We have
from time to time experienced, and may in the future experience, shortages of certain types of qualified employees.

 If
we lose the services of any executive officers or key employees, our ability to achieve our business objectives could be harmed, which
in turn could adversely affect our business, financial condition and results of operations. We have and may continue to experience turnover
in certain executive officer and key employee roles.

 Business
interruptions could limit our ability to operate our business.

 Our
operations, as well as those of the collaborators on which we depend, are vulnerable to damage or interruption from fire, natural disasters,
including earthquakes, weather related supply chain delivery disruptions, computer viruses, cyber-attacks, human error, power shortages,
telecommunication failures, international acts of terror, foreign or domestic conflicts, epidemics or pandemics such as the COVID-19
pandemic, and other similar events. Although we have certain business continuity plans in place, we have not established a formal comprehensive
disaster recovery plan, and our back-up operations and business interruption insurance may not be adequate to compensate us for losses
we may suffer. A significant business interruption could result in losses or damages incurred by us and require us to cease or curtail
our operations.

 The
operation of Aspira Labs and our Aspira Synergy business depends on the effectiveness and availability of our information systems, including
the information systems we use to provide services to our customers and to store employee data. If our information technology systems
or those third parties upon which we rely or our data, are or were compromised, we could experience adverse consequences resulting from
such compromise, including but not limited to regulatory investigations or actions; litigation; fines and penalties; disruptions of our
business operations; reputational harm; loss of revenue or profits; loss of customers or sales; and other adverse consequences.

 In
the ordinary course of our business, we and the third parties upon which we rely, process, collect, receive, store, use, transfer, make
accessible, and share (collectively, processing) proprietary, confidential, and sensitive data, including personal data (such as health-related
data), intellectual property, trade secrets and other sensitive data the Company may process (collectively, sensitive information).

 The
information systems we use for our Aspira Labs business are comprised of systems we have purchased or developed, our legacy information
systems and, increasingly, web-enabled and other integrated information systems. In using these information systems, we may rely on third-party
vendors to provide hosting services, where our infrastructure is dependent upon the reliability of their underlying platforms, facilities
and communications systems.

 As
the breadth and complexity of Aspira Labs' information system grows, we will be increasingly exposed to the risks inherent in maintaining
the stability of our legacy systems due to prior customization, attrition of employees or vendors involved in their development, and
obsolescence of the underlying technology as well as risks from the increasing number and scope of external data breaches on companies
generally. Because certain customers and clinical trials may be dependent upon these legacy systems, we will also face an increased level
of risk in maintaining the legacy systems and limited options to mitigate such risk. We are also exposed to risks associated with the
availability of all of our information systems, including

 discontinued
 vendor support of legacy systems;

 disruption,
 impairment or failure of data centers, telecommunications facilities or other key infrastructure platforms, including those maintained
 by third-party vendors;

 failures
 or malfunctions in our internal systems, including our employee data and communications, critical application systems and their associated
 hardware; and

 excessive
 costs, excessive delays and other deficiencies in systems development and deployment.

 Cyber-attacks,
malicious internet-based activity, online and offline fraud, social-engineering attacks (including through deep fakes, which may be increasingly
more difficult to identify as fake, and phishing attacks), malicious code (such as viruses and worms), malware (including as a result
of advanced persistent threat intrusions), denial-of-service attacks, credential stuffing, credential harvesting, personnel misconduct
or error, ransomware attacks, supply-chain attacks, software bugs, server malfunctions, software or hardware failures, loss of data or
other information technology assets, adware, attacks enhanced or facilitated by AI, telecommunications failures, and other similar activities
or incidents threaten the confidentiality, integrity, and availability of our sensitive information and information technology systems,
and those of the third parties upon which we rely. Such threats are prevalent and continue to rise, are increasingly difficult to detect,
and come from a variety of sources, including traditional computer "hackers," threat actors, "hacktivists," organized
criminal threat actors, personnel (such as through theft or misuse), sophisticated nation states, and nation-state-supported actors.
In addition to experiencing a security incident, third parties may gather, collect, or infer sensitive information about us from public
sources, data brokers, or other means that reveals competitively sensitive details about our organization and could be used to undermine
our competitive advantage or market position.

 The
materialization of any of these risks may impede the processing of data, the delivery of databases and services, and the day-to-day management
of our Aspira Labs business and could result in the corruption, loss or unauthorized disclosure of proprietary, confidential or other
data. In particular, severe ransomware attacks are becoming increasingly prevalent and can lead to significant interruptions in our operations,
ability to provide our products or services, loss of sensitive data and income, reputational harm, and diversion of funds. Extortion
payments may alleviate the negative impact of a ransomware attack, but we may be unwilling or unable to make such payments due to, for
example, applicable laws or regulations prohibiting such payments. Further, remote work has become more common and has increased risks
to our information technology systems and data, as more of our employees utilize network connections, computers and devices outside our
premises or network, including working at home, while in transit and in public locations.

 Our
mitigation efforts to date might not adequately protect us in the event of a system failure, cyber-attack, cyber-breach, data breach
or other adverse event. Despite any precautions we take, damage from fire, floods, hurricanes, the outbreak or escalation of war, acts
of terrorism, power loss, telecommunications failures, computer viruses, break-ins and similar events at our various computer facilities
or those of our third-party vendors could result in interruptions in the flow of data to us and from us to our customers. Corruption
or loss of data may result in the need to repeat a trial at no cost to the customer, but at significant cost to us, the termination of
a contract or damage to our reputation. As our business continues its efforts to expand globally, these types of risks may be further
increased by instability in the geopolitical climate of certain regions, underdeveloped and less stable utilities and communications
infrastructure, and other local and regional factors. Additionally, significant delays in system enhancements or inadequate performance
of new or upgraded systems could damage our reputation and harm our business.

 Unauthorized
disclosure of sensitive or confidential data, whether through systems failure or employee or distributor negligence, cyber-attacks, fraud
or misappropriation, could damage our reputation and cause us to lose customers and, to the extent any such unauthorized disclosure compromises
the privacy and security of individually identifiable health information, could also cause us to face sanctions and fines under HIPAA
of 1996 as amended by HITECH. Similarly, we have been and expect that we will continue to be subject to attempts to gain unauthorized
access to or through our information systems or those we internally or externally develop for our customers, including a cyber-attack
by computer programmers and hackers who may develop and deploy viruses, worms or other malicious software programs, process breakdowns,
denial-of-service attacks, malicious social engineering or other malicious activities, or any combination of the foregoing. These same
risks also apply to Aspira Labs. Successful attacks could result in negative publicity, significant remediation and recovery costs, legal
liability and damage to our reputation and could have an adverse effect on our business, financial condition and results of operations.

 We
use AI/ML to assist us in making certain decisions, which is regulated by certain privacy laws. Due to inaccuracies or flaws in the inputs,
outputs, or logic of the AI/ML, the model could be biased and could lead us to make decisions that could bias certain individuals (or
classes of individuals), and adversely impact their rights, employment, and ability to obtain certain pricing, products, services, or
benefits.

 Our
contracts may not contain limitations of liability, and even where they do, there can be no assurance that limitations of liability in
our contracts are sufficient to protect us from liabilities, damages, or claims related to our data privacy and security obligations.
We cannot be sure that our insurance coverage will be adequate or sufficient to protect us from or to mitigate liabilities arising out
of our privacy and security practices, that such coverage will continue to be available on commercially reasonable terms or at all, or
that such coverage will pay future claims.

 We
selectively explore acquisition opportunities and strategic alliances relating to other businesses, products or technologies. We may
not be successful in integrating other businesses, products or technologies with our business. Any such transaction also may not produce
the results we anticipate, which could adversely affect our business, financial condition and results of operations.

 We
selectively explore and may pursue acquisition and other opportunities to strengthen our business and grow our company. We may enter
into business combination transactions, make acquisitions or enter into strategic partnerships, joint ventures or alliances, any of which
may be material. The market for acquisition targets and strategic alliances is highly competitive, which could make it difficult to find
appropriate merger or acquisition opportunities. If we are required to raise capital by incurring debt or issuing additional equity for
any reason in connection with a strategic acquisition or investment, financing may not be available or the terms of such financing may
not be favorable to us and our stockholders, whose interests may be diluted by the issuance of additional stock.

 The
process of integration may produce unforeseen regulatory issues and operating difficulties and expenditures and may divert the attention
of management from the ongoing operation of our business and harm our reputation. We may not successfully achieve the integration objectives,
and we may not realize the anticipated cost savings, revenue growth and synergies in full or at all, or it may take longer to realize
them than expected, any of which could negatively impact our business, financial condition and results of operations.

 Future
litigation by or against us could be costly and time-consuming to prosecute or defend.

 We
are from time to time subject to legal proceedings and claims that arise in the ordinary course of business, such as claims brought by
our clients in connection with commercial disputes, employment claims made by current or former employees, and claims brought by third
parties alleging infringement of their intellectual property rights. In addition, we may bring claims against third parties for infringement
of our intellectual property rights. Litigation may result in substantial costs and may divert our attention and resources, which may
adversely affect our business, results of operations and financial condition.

 An
unfavorable judgment against us in any legal proceeding or claim could require us to pay monetary damages. In addition, an unfavorable
judgment in which the counterparty is awarded equitable relief, such as an injunction, could harm our business, results of operations
and financial condition.

 RISKS
RELATED TO OWNING OUR STOCK

 If
we fail to maintain compliance with the Nasdaq minimum listing requirements, our common stock will be subject to delisting. Our ability
to publicly or privately sell equity securities and the liquidity of our common stock could be adversely affected if our common stock
is delisted.

 Our
common stock is currently listed on The Nasdaq Capital Market. T he continued listing of our common stock on The Nasdaq Capital Market is contingent
on our continued compliance with a number of listing requirements. If we are unable to comply with the continued listing requirements
of The Nasdaq Capital Market, our common stock would be delisted from The Nasdaq Capital Market, which would limit investors'
ability to effect transactions in our common stock and subject us to additional trading restrictions. In order to maintain
our listing, we must maintain certain share prices, financial and share distribution targets, including maintaining a minimum amount
of stockholders' equity and a minimum number of public stockholders, as well as satisfying other listing requirements of The Nasdaq
Capital Market. In addition to these objective standards, The Nasdaq Capital Market may delist the securities of any issuer for other
reasons involving the judgment of The Nasdaq Capital Market.

 On
July 1, 2024, we received a deficiency letter (the "Notice") from the Listing Qualifications Department of The Nasdaq Stock
Market, LLC ("Nasdaq") stating that for the 30 consecutive business days prior to the date of the Notice, our Market Value
of Listed Securities was below the minimum of $35 million required for continued listing on Nasdaq pursuant to Nasdaq Listing Rule 5550(b)(2)
(the "MVLS Requirement"). To regain compliance with the MVLS Requirement, the market value of our common stock must have
met or exceeded $35.0 million for a minimum of 10 consecutive business days during the 180-day grace period ending on December 30, 2024
(the "MVLS Compliance Date"), unless the Staff of Nasdaq exercises its discretion to extend this 10 consecutive business
day period. As of December 30, 2024, we were unable to regain compliance by the MVLS Compliance Date. As such, on December 31,
2024, Nasdaq notified us that our securities are subject to delisting. While we requested an appeal of Nasdaq's delisting
determination and presented our plan at a hearing on February 18, 2025, no assurance can be provided that we will be successful in appealing
such determination and maintaining the listing of our common stock on The Nasdaq Capital Market.

 We
presented an appeal of Nasdaq's determination to delist our common stock. As a result of the hearing, on March 6, 2025, we received
written notice from Nasdaq that it would grant our request for continued listing on the Nasdaq Capital Market subject to certain conditions.
Although we have been granted the conditional exception to remain listed on the Nasdaq Capital Market, no assurance can be provided that
we will successfully meet the conditions of the exception and that our common stock will continue to be listed on The Nasdaq Capital
Market.

 Furthermore,
on October 17, 2024, we received written notice from Nasdaq that we were not in compliance with Nasdaq Listing Rule 5550(a)(2), as the
minimum bid price of our common stock had been below $1.00 per share for 30 consecutive business days. In accordance with Nasdaq Listing
Rule 5810, and assuming our common stock is not delisted for our failure to satisfy the MVLS Requirement by the MVLS Compliance Date,
we will have a period of 180 calendar days, or until April 15, 2025, to regain compliance with the minimum bid price requirement and
market value of common stock requirement. To regain compliance with the Nasdaq bid price requirement, the closing bid price of our common
stock must meet or exceed $1.00 per share for at least 10 consecutive business days during this 180- calendar day period. In the event
we do not regain compliance by April 15, 2025, we may be eligible for an additional 180 calendar day grace period.

 On February 11, 2025, we received
written notice from the Nasdaq Stock Market, LLC that based on the closing bid price per share immediately preceding entering into a binding
agreement to issue the securities for the Private Placement of $1.47 per share plus $0.125 attributable to the value of the warrants,
the market value of the transaction for purposes of Listing Rule 5625(c) was $1.595. Since the shares and warrants sold in the private
placement were issued below the market value, and we failed to obtain shareholder approval, we violated Listing Rule 5635(c). Accordingly,
this matter served as an additional basis for delisting our securities from The Nasdaq Stock Market.

 Subsequently,
on February 11, 2025, we completed amendments to the warrants prohibiting exercise until shareholder approval has been obtained. As a
result, the Staff of Nasdaq determined that we had regained compliance with Listing Rule 5635(c).

 There
is no assurance that we will be able to maintain compliance with The Nasdaq Capital Market continued listing standards and/or continue
our listing on The Nasdaq Capital Market in the future.

 If
the Nasdaq Capital Market delists our securities from trading on its exchange and we are not able to list our securities on another national
securities exchange, we expect our securities would qualify to be quoted on an over-the-counter market. If this were to occur, we could
face significant material adverse consequences, including:

 a
 limited availability of market quotations for our securities;

 reduced
 liquidity for our securities;

 substantially
 impair our ability to raise additional funds;

 the
 loss of institutional investor interest and a decreased ability to issue additional securities or obtain additional financing in
 the future;

 a
 determination that our common stock is a "penny stock," which will require brokers trading in our common stock to adhere
 to more stringent rules and possibly result in a reduced level of trading activity in the secondary trading market for our securities;

 a
 limited amount of news and analyst coverage; and

 potential
 breaches of representations or covenants of our agreements pursuant to which we made representations or covenants relating to our
 compliance with applicable listing requirements, which, regardless of merit, could result in costly litigation, significant liabilities
 and diversion of our management's time and attention and could have a material adverse effect on our financial condition, business
 and results of operations.

 Unless
our common stock continues to be listed on a national securities exchange it will become subject to the so-called "penny stock"
rules that impose restrictive sales practice requirements.

 If
we are unable to maintain the listing of our common stock on the Nasdaq Capital Market or another national securities exchange, our common
stock could become subject to the so-called "penny stock" rules if the shares have a market value of less than $5.00 per
share. The SEC has adopted regulations that define a penny stock to include any stock that has a market price of less than $5.00 per
share, subject to certain exceptions, including an exception for stock traded on a national securities exchange. The SEC regulations
impose restrictive sales practice requirements on broker-dealers who sell penny stocks to persons other than established customers and
accredited investors. For transactions covered by this rule, the broker-dealer must make a special suitability determination for the
purchaser and must have received the purchaser's written consent to the transaction prior to sale. This means that if we are unable
to maintain the listing of our common stock on a national securities exchange, the ability of stockholders to sell their common stock
in the secondary market could be adversely affected. If a transaction involving a penny stock is not exempt from the SEC's rule,
a broker-dealer must deliver a disclosure schedule relating to the penny stock market to each investor prior to a transaction. The broker-dealer
also must disclose the commissions payable to both the broker-dealer and its registered representative, current quotations for the penny
stock, and, if the broker-dealer is the sole market-maker, the broker-dealer must disclose this fact and the broker-dealer's presumed
control over the market. Finally, monthly statements must be sent disclosing recent price information for the penny stock held in the
customer's account and information on the limited market in penny stocks.

 The
liquidity and trading volume of our common stock may be low, and our ownership is concentrated, which could adversely impact the trading
price of our common stock and our stockholders' ability to obtain liquidity.

 The
liquidity and trading volume of our common stock has at times been low in the past and may again be low in the future. If the liquidity
and trading volume of our common stock is low, this could adversely impact the trading price of our common stock and our stockholders'
ability to obtain liquidity in their shares of our common stock.

 In
addition, pursuant to a stockholders agreement we entered into in connection with a May 2013 private placement, one of our stockholders
has the right to designate a director to be nominated by us to serve on our board of directors. Furthermore, this stockholder agreement
gives two investors the right to participate in future equity offerings, on the same terms as other investors. In addition, the stockholders
agreement prohibits us from taking certain material actions without the consent of at least one of the primary investors in the May 2013
private placement. These material actions include:

 making
 any acquisition with a value greater than $2 million;

 offering,
 selling or issuing securities senior to our common stock or any securities that are convertible into or exchangeable or exercisable
 for securities ranking senior to our common stock;

 taking
 any action that would result in a change in control of the Company or an insolvency event; and

 paying
 or declaring dividends on any of our securities or distributing any of our assets other than in the ordinary course of business or
 repurchasing any of our outstanding securities.

 The
foregoing rights terminate for a primary investor when that investor ceases to beneficially own less than 50% of the shares and warrants
(taking into account shares issued upon exercise of the warrants), in the aggregate, that were purchased at the closing of the 2013 private
placement. We believe that the rights of one of the primary investors have terminated. The interests of the parties to the stockholders
agreement could conflict with or differ from our interests or the interests of other stockholders.

 As
a result of the foregoing, a limited number of stockholders will be able to affect the outcome of, or exert significant influence over,
all matters requiring stockholder approval, including the election and removal of directors and any change in control involving us. In
addition, this concentration of ownership of our common stock could have the effect of delaying or preventing a change in control of
us or otherwise discouraging or preventing a potential acquirer from attempting to obtain control of us. This, in turn, could have a
negative effect on the market price of our common stock. It could also prevent our stockholders from realizing a premium over the market
prices for their shares of common stock. Moreover, the interests of this concentration of ownership may not always coincide with our
interests or the interests of other stockholders. In addition, the interests of the parties to the stockholders agreement could conflict
with or differ from our interests or the interests of other stockholders. The concentration of ownership also contributes to the low
trading volume and volatility of our common stock.

 Our
stock price has been, and may continue to be, highly volatile.

 The
trading price of our common stock has been highly volatile. Between January 1, 2024, and December 31, 2024, the
closing trading price of our common stock ranged from $5.63 to $0.70. The trading price of our common stock could continue to be subject
to wide fluctuations in price in response to various factors, many of which are beyond our control, including:

 failure
 to significantly increase revenue and volumes of OvaSuite or Aspira Synergy;

 actual
 or anticipated period-to-period fluctuations in financial results;

 failure
 to achieve, or changes in, financial estimates by securities analysts;

 announcements
 or introductions of new products or services or technological innovations by us or our competitors;

 failure
 to complete clinical studies that validate clinical utility sufficiently to increase positive medical policy among payers at large;

 publicity
 regarding actual or potential discoveries of biomarkers by others;

 comments
 or opinions by securities analysts or stockholders;

 the
 ability to maintain the listing of our securities on The Nasdaq Capital Market;

 conditions
 or trends in the pharmaceutical, biotechnology or life science industries;

 announcements
 by us of significant acquisitions and divestitures, strategic partnerships, joint ventures or capital commitments;

 developments
 regarding our patents or other intellectual property or that of our competitors;

 litigation
 or threat of litigation;

 additions
 or departures of key personnel;

 limited
 daily trading volume;

 our
 ability to continue as a going concern;

 economic
 and other external factors, disasters or crises; and

 our
 announcement of future fundraisings.

 In
addition, the stock market in general and the market for diagnostic technology companies, in particular, have experienced significant
price and volume fluctuations that have often been unrelated or disproportionate to the operating performance of those companies. These
broad market and industry factors may adversely affect the market price of our securities, regardless of our operating performance. In
the past, following periods of volatility in the market price of a company's securities, securities class action litigation has
often been instituted. A securities class action suit against us could result in substantial costs, potential liabilities and the diversion
of our attention and our resources.

 Anti-takeover
provisions in our charter, bylaws, other agreements and under Delaware law could make a third-party acquisition of the Company difficult.

provisions of our certificate of incorporation and bylaws may have the effect of making it more difficult for a third party to acquire,
or of discouraging a third party from attempting to acquire, control of us, even if a change of control might be deemed beneficial to
our stockholders. Such provisions could limit the price that certain investors might be willing to pay in the future for our securities.
Our certificate of incorporation eliminates the right of stockholders to call special meetings of stockholders or to act by written consent
without a meeting, and our bylaws require advance notice for stockholder proposals and director nominations, which may preclude stockholders
from bringing matters before an annual meeting of stockholders or from making nominations for directors at an annual meeting of stockholders.
Our certificate of incorporation authorizes undesignated preferred stock, which makes it possible for our board of directors, without
stockholder approval, to issue preferred stock with voting or other rights or preferences that could adversely affect the voting power
of holders of common stock. In addition, the likelihood that the holders of preferred stock will receive dividend payments and payments
upon liquidation could have the effect of delaying, deferring or preventing a change in control.

 In
connection with our private placement offering of common stock and warrants in May 2013 we entered into a stockholders agreement (the
"2013 Stockholders Agreement") which, among other things, includes agreements limiting our ability to effect a change in
control without the consent of at least one of the primary investors in that offering. These and other provisions may have the effect
of deferring hostile takeovers or delaying changes in control or management of us. The amendment of any of the provisions of either our
certificate of incorporation or bylaws described in the preceding paragraph would require not only approval by our board of directors
and the affirmative vote of at least 66 2/3% of our then outstanding voting securities, but also consent pursuant to the terms of the
2013 Stockholders Agreement. We are also subject to certain provisions of Delaware law that could delay, deter or prevent a change in
control of the Company. These provisions could make a third-party acquisition of the Company difficult and limit the price that investors
might be willing to pay in the future for shares of our common stock.

 If
we raise additional capital in the future, your ownership in us could be diluted.

 In
order to raise additional capital, we may offer additional shares of common stock or other securities convertible into or exchangeable
for our common stock. We may sell shares or other securities in any other offering at a price per share that is less than the price for
securities paid by investors in previous offerings, and investors purchasing shares or other securities in the future could have rights
superior to existing stockholders. The price per share at which we sell additional shares of common stock or securities convertible into
common stock in future transactions may be higher or lower than the price for securities offered in previous offerings.

 Until
such time, if ever, as we can generate substantial revenue from our operations, we anticipate financing our cash needs through a combination
of equity offerings, debt financings and license agreements. To the extent that we raise additional capital through the further sale
of equity securities or convertible debt securities, your ownership interest will be diluted.

 Sales
of a substantial number of our shares of common stock in the public market could cause our stock price to fall.

 We
may issue and sell additional shares of common stock in the public markets. Sales of a substantial number of shares of our common stock
in the public markets or the perception that such sales could occur could depress the market price of our securities and impair our ability
to raise capital through the sale of additional equity securities.

 Because
we do not currently intend to declare cash dividends on our shares of common stock in the foreseeable future, stockholders must rely
on appreciation of the value of our common stock for any return on their investment.

 We
have never declared or paid cash dividends on our capital stock. We currently intend to retain all of our future earnings, if any, to
finance the operation, development and growth of our business. Furthermore, any future debt agreements may also preclude us from paying
or place restrictions on our ability to pay dividends. As a result, capital appreciation, if any, of our common stock will be your sole
source of gain with respect to your investment for the foreseeable future.

 The
exercise of our outstanding options and warrants will dilute stockholders and could decrease our stock price.

 The
exercise of our outstanding options and warrants may adversely affect our stock price due to sales of a large number of shares or the
perception that such sales could occur. These factors also could make it more difficult to raise funds through future offerings of our
securities, and could adversely impact the terms under which we could obtain additional equity capital. Exercise of outstanding options
and warrants or any future issuance of additional shares of common stock or other equity securities, including, but not limited to, options,
warrants, restricted stock units or other derivative securities convertible into our common stock, may result in significant dilution
to our stockholders and may decrease our stock price.

 ITEM
 1B.

 UNRESOLVED
 STAFF COMMENTS

 None.

 ITEM
 1C.

 CYBERSECURITY

 Risk
Management and Strategy

 We
have implemented and maintain various information security processes designed to identify, assess and manage material risks from cybersecurity
threats to our critical computer networks, third party hosted services, communications systems, hardware and software, and our critical
data, including intellectual property, confidential information that is proprietary, strategic or competitive in nature, and trade secrets,
data we may collect about trial participants in connection with clinical trials, sensitive third-party data, business plans, transactions,
and financial information ("Information Systems and Data").

 Our
cybersecurity function, which comprises, in part, our IT department, legal team, human resources team and our audit committee, helps
identify, assess and manage our cybersecurity threats and risks. Our cybersecurity function identifies and assesses risks from cybersecurity
threats by monitoring and evaluating our threat environment using various methods including, for example, automated tools, subscribing
to and analyzing reports and services that identify cybersecurity threats, conducting vulnerability assessments to identify vulnerabilities,
and evaluating threats reported to us.

 Depending
on the environment, we implement and maintain various technical, physical, and organizational measures and processes designed to manage
and mitigate material risks from cybersecurity threats to our Information Systems and Data, including, for example: incident detection
and response, data encryption, network security controls, employee training, access controls, physical security, systems monitoring,
and asset management, tracking, and disposal.

 Our
assessment and management of material risks from cybersecurity threats are integrated into our overall risk management processes. For
example, the cybersecurity function works with management to prioritize our risk management processes and mitigate cybersecurity threats
that are more likely to lead to a material impact to our business.

 We
use third-party service providers to assist us from time to time to identify, assess, and manage material risks from cybersecurity threats,
including for example professional services firms (including legal counsel) and cybersecurity consultants. We also use third-party service
providers to perform a variety of functions throughout our business, such as hosting companies, application providers, and supply chain
resources. We manage cybersecurity risks associated with our use of these providers by, for example, requesting and analyzing responses
on a security questionnaire and conducting audits and risk assessments on certain vendors. In particular, our legal department performs
an assessment on each vendor and based on certain criteria will have our IT team perform a security assessment.

 For
a description of the risks from cybersecurity threats that may materially affect the Company and how they may do so, see our risk factors
under Part 1. Item 1A. Risk Factors in this Annual Report on Form 10-K, including "The operation of Aspira Labs and our Aspira
Synergy business depends on the effectiveness and availability of our information systems, including the information systems we use to
provide services to our customers and to store employee data, and failures of these systems, including in connection with cyber-attacks,
may materially limit our operations or have an adverse effect on our reputation."

 Governance

 Our
board of directors addresses our cybersecurity risk management as part of its general oversight function. The Audit Committee is responsible
for overseeing our cybersecurity risk management processes, including oversight and mitigation of risks from cybersecurity threats.

 Our
cybersecurity risk assessment and management processes are implemented and maintained by certain members of our management, including
our Manager of IT Infrastructure, who has over 20 years of experience in various IT administration roles, five of which have been in
cybersecurity.

 Our
HR department is responsible for hiring appropriate personnel, helping to integrate cybersecurity risk considerations into our overall
risk management strategy, and communicating key priorities to relevant personnel. Our CFO, working with our Manager of IT Infrastructure,
is responsible for approving budgets, helping prepare for cybersecurity incidents, approving cybersecurity processes, and reviewing security
assessments and other security-related reports. Our legal department is also responsible for performing a cyber risk assessment on each
new vendor.

 Our
response process to cybersecurity incidents is designed to escalate certain incidents to members of management depending on the circumstances,
including our Manager of IT Infrastructure. Our Manager of IT Infrastructure works with our incident response team to help us to mitigate
and remediate cybersecurity incidents of which they are notified. In addition, our incident response policy includes reporting to the
board of directors committee responsible for certain cybersecurity incidents.

 The
Audit Committee receives periodic reports from our cybersecurity function concerning our significant cybersecurity threats and risk and
the processes we have implemented to address them. The Audit Committee also has access to various reports, summaries or presentations
related to cybersecurity threats, risk and mitigation.

 ITEM
 2.

 PROPERTIES

 The
following chart indicates the facilities that we lease, the location and size of each facility and its designated use. We believe that
these facilities are suitable and adequate for our current needs.

 Location

 Approximate
 Square Feet

 Primary
 Functions

 Lease
 Expiration Date

 Austin,
 Texas

 8,203
 sq. ft.

 Labs facility, research and development, clinical and regulatory and administrative offices

 31, 2031

 Shelton,
 Connecticut

 4,614
 sq. ft.

 Administrative
 offices

 September
 30, 2028

 ITEM
 3.

 LEGAL
 PROCEEDINGS

 From
time to time, we are involved in legal proceedings and regulatory proceedings arising out of our operations. We establish reserves for
specific liabilities in connection with legal actions that we deem to be probable and estimable. As of the date of the filing of this
Form 10-K, we are not a party to any proceeding, the adverse outcome of which would have a material adverse effect on our financial position
or results of operations.

 ITEM
 4.

 MINE
 SAFETY DISCLOSURES

 Not
applicable.

 PART
II

 ITEM
 5.

 MARKET
 FOR REGISTRANT'S COMMON EQUITY, RELATED STOCKHOLDER MATTERS AND ISSUER PURCHASES OF EQUITY SECURITIES

 Market
Information

 Our
common stock is traded on The Nasdaq Capital Market under the symbol "AWH."

 Holders
of Common Stock

 On
March 25, 2025, there were 45 registered holders of record of our common stock.

 Dividends

 We
have never paid or declared any dividend on our common stock and we do not anticipate paying cash dividends on our common stock in the
foreseeable future. If we pay a cash dividend on our common stock, we also may be required to pay the same dividend on an as-converted
basis on any outstanding warrants or other securities. Moreover, any preferred stock or other senior debt or equity securities to be
issued and any future credit facilities might contain restrictions on our ability to declare and pay dividends on our common stock. We
intend to retain all available funds and any future earnings to fund the development and expansion of our business.

 Recent
Sales of Unregistered Securities

 None.

 Issuer
Purchases of Equity Securities

 None.

 Equity
Compensation Plan Information

 Information
about our equity compensation plans is incorporated herein by reference to Item 12 of Part III of this Annual Report on Form 10-K

 Stock
Performance Graph

 We
are a smaller reporting company as defined by Rule 12b-2 of the Exchange Act and are not required to provide the information required
under this item.

 ITEM
 6.

 [Reserved]

 ITEM
 7.

 MANAGEMENT'S
 DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS

 You
should read the following discussion and analysis in conjunction with our audited Consolidated Financial Statements and related Notes
thereto, included on pages F-1 through F-29 in this Annual Report on Form 10-K. The statements below contain forward-looking statements
based upon current plans, expectations, and beliefs that involve risks and uncertainties. Actual results may differ materially from those
contained in any forward-looking statement, due to a number of factors, including those discussed in the section of this Annual Report
on Form 10-K entitled "Forward-Looking Statements" and "Item 1A. Risk Factors" in this Form 10-K. You should
read these sections carefully.

 Overview

 We
are dedicated to the discovery, development, and commercialization of noninvasive, AI-powered tests to aid in the diagnosis of gynecologic
diseases, starting with ovarian cancer.

 We
plan to broaden our focus to the differential diagnosis of other gynecologic diseases that typically cannot be assessed through traditional
non-invasive clinical procedures. We expect to continue commercializing our existing and new technology and to distribute our tests through
our decentralized technology transfer service platform, Aspira Synergy. We also intend to continue to raise public awareness regarding
the diagnostic superiority of the Ova1Plus workflow as compared to CA-125 on its own for all women with adnexal masses, as well as the
superior performance of machine learning algorithms in detecting ovarian cancer in different racial and ethnic populations. We plan to
continue to expand access to our tests among Medicaid patients as part of our corporate mission to make the best care available to all
women, and we plan to advocate for legislation and the adoption of our technology in professional society guidelines to provide broad
access to our products and services.

 We
are focused on commercializing our products and have established medical and advisory support and a Key Opinion Leader Network aligned
with our territories in the U.S. In addition, we added to our direct salesforce, and in 2021, we put Ova1 on our global testing platform,
Aspira Synergy. This platform allows tests to be deployed internationally as well as run by clients in the United States at major customer
sites. In 2024, we plan to continue our efforts to commercialize the Ova1Plus workflow by utilizing select partnerships for distribution
and expanding our managed care coverage and contracts in select markets.

 To
continue our commercialization objectives and reach our financial and operational goals, we require skilled sales individuals with familiarity
in our industry. We have from time to time experienced, and may in the future experience, shortages of certain types of qualified employees.

 Critical
Accounting Policies and Estimates

 Our
significant accounting policies are described in Note 1, Basis for Presentation and Summary of Significant Accounting and Reporting Policies,
of the Notes to the Consolidated Financial Statements included in this Annual Report on Form 10-K. The Consolidated Financial Statements
are prepared in conformity with GAAP. Preparation of the financial statements requires us to make critical judgments, estimates, and
assumptions that affect the amounts of assets and liabilities in the financial statements and revenues and expenses during the reporting
periods (and related disclosures). We believe the policies discussed below are our critical accounting estimates, as they include the
more significant, subjective, and complex judgments and estimates made when preparing our consolidated financial statements.

 Revenue
Recognition

 We
recognize product revenue in accordance with the provisions of ASC Topic 606, Revenue from Contracts with Customers ("ASC
606"); all revenue is recognized upon completion of the OvaSuite tests based on estimates of amounts that will
ultimately be realized. In determining the amount to accrue for a delivered test result, we consider factors such as historical payment
history and amount, payer coverage, whether there is a reimbursement contract between the payer and us, and any current developments
or changes that could impact reimbursement. These estimates are subject to uncertainty and require significant judgment by management
because of the various inputs of the factors considered. We also review our patient account population and determine an appropriate distribution
of patient accounts by payer ( i.e., Medicare, patient pay, other third-party payer, etc.) into portfolios with similar
collection experience. When evaluated for collectability, this results in a materially consistent revenue amount for such portfolios
as if each patient account were evaluated on an individual contract basis.

 Stock-Based
Compensation

 We
record the fair value of non-cash stock-based compensation costs for stock options and stock purchase rights related to the 2010 and
2019 Plans. We estimate the fair value of stock options using a Black-Scholes option valuation model. This model requires the input of
subjective assumptions including expected stock price volatility, expected life and estimated forfeitures of each award. We use the straight-line
method to amortize the fair value over the vesting period of the award. These assumptions consist of estimates of future market conditions,
which are inherently uncertain, and therefore are subject to management's judgment.

 The
expected life of options is based on historical data of our actual experience with the options we have granted and represents the period
of time that the options granted are expected to be outstanding. This data includes employees' expected exercise and post-vesting
employment termination behaviors. The expected stock price volatility is estimated using our historical volatility in deriving the expected
volatility assumption. We made an assessment that our historic volatility is most representative of future stock price trends. The expected
dividend yield is based on the estimated annual dividends that we expect to pay over the expected life of the options as a percentage
of the market value of our common stock as of the grant date. The risk-free interest rate for the expected life of the options granted
is based on the United States Treasury yield curve in effect as of the grant date.

 There
is inherent uncertainty in our forecasts and projections and, if we had made different assumptions and estimates than those described
previously, the amount of our stock-based compensation expense, net loss and net loss per common stock amounts could have been materially
different.

 Liquidity

 As
discussed in Note 1 to the consolidated financial statements, we have incurred significant net losses and negative cash flows from operations
since inception, and as a result have an accumulated deficit of approximately $531,397,000 at December 31, 2024. We expect to incur a
net loss in 2025 as well. In order to continue our operations as currently planned through 2025 and beyond, we will need to raise additional
capital. Given the above conditions, there is substantial doubt about our ability to continue as a going concern. The consolidated financial
statements have been prepared on a going concern basis and do not include any adjustments that might result from these uncertainties.

 Recent
Accounting Pronouncements

 Refer
to Note 2 in our consolidated financial statements contained in Part II, Item 8, "Consolidated Financial Statements and Supplementary
Data," of this Annual Report on Form 10-K.

 Results
of Operations – Year Ended December 31, 2024 as compared to Year Ended December 31, 2023

 Our
selected summary financial and operating data for the years ended December 31, 2024 and 2023 were as follows:

 Increase (Decrease)

 (dollars in thousands)

 2024

 2023

 Amount

 %

 Revenue:

 $
 9,182

 $
 9,153

 $

 -

 Genetics

 -

 (1
)

 -

 Total revenue

 9,182

 9,154

 -

 Cost of revenue:

 3,703

 3,892

 (189
)

 (5
)

 Genetics

 -

 -

 -

 -

 Total cost of revenue

 3,703

 3,892

 (189
)

 (5
)

 Gross profit

 5,479

 5,262

 Operating expenses:

 3,266

 4,035

 (769
)

 (19
)

 8,146

 7,812

 10,345

 12,267

 (1,922
)

 (16
)

 Total operating expenses

 21,757

 24,114

 (2,357
)

 (10
)

 Loss from operations

 (16,278
)

 (18,852
)

 2,574

 (14
)

 Other income (expense), net:

 Change in fair value of warrant liabilities

 1,346

 Interest income (expense), net

 (33
)

 (81
)

 (169
)

 Forgiveness of DECD loan

 -

 1,000

 (1,000
)

 -

 Other income, net

 1,871

 1,386

 Total other income, net

 3,184

 2,162

 1,022

 $
 (13,094
)

 $
 (16,690
)

 $
 3,596

 (22
)

Revenue. Product revenue was $9,182,000 for the year ended December 31, 2024, compared to $9,153,000 for the same period in 2023.
Revenue is recognized when the test result is successfully delivered and is based on estimates of what we expect to ultimately realize.

 The
number of OvaSuite tests performed increased 1% to approximately 24,305 tests during the year ended December 31, 2024 compared to approximately
23,990 OvaSuite tests for the same period in 2023.

 The
volume and AUP for the year ended December 31, 2024 and 2023 were as follows:

 2024

 2023

 Product Volume:

 Ova1Plus

 19,202

 20,579

 OvaWatch

 5,103

 3,411

 Total OvaSuite

 24,305

 23,990

 Average Unit Price (AUP):

 Ova1Plus

 $

 $

 OvaWatch

 Total OvaSuite

 $

 $

 Cost
of Revenue – Product. Cost of product revenue was $3,703,000 for the year ended December 31, 2024 compared to $3,892,000
for the same period in 2023, representing a decrease of $189,000, or 5%. The decrease was primarily due to a decrease in consulting costs
and lab supplies, offset by an increase in shipping costs. We expect the cost of product to increase slightly in 2025 as the number of
tests performed continues to grow.

 Research
and Development Expenses. Research and development expenses represent costs incurred to develop our technology and carry out
clinical studies, and include personnel-related expenses, regulatory costs, reagents and supplies used in research and development laboratory
work, infrastructure expenses, contract services and other outside costs. Research and development expenses for the year ended December
31, 2024 decreased by $769,000, or 19%, compared to the same period in 2023. This decrease was primarily due to a decrease in employment-related
expenses of approximately $789,000 and a decrease in clinical trials of $227,000, offset by an increase to our lab supplies of $132,000,
as well as a one-time credit in 2023 related to collaborations of $200,000. We expect research and development expenses to decrease further
in 2025 due to recent personnel changes.

 Sales
and Marketing Expenses. Our sales and marketing expenses consist primarily of personnel-related expenses, education and promotional
expenses. These expenses include the costs of educating physicians and other healthcare professionals, medical meeting participation,
and dissemination of scientific and health economic publications. Sales and marketing expenses for the year ended December 31, 2024 increased
by $334,000, or 4%, compared to the same period in 2023. This increase was primarily due to costs
related to our contracted sales team of $740,000, increased personnel costs of $175,000 and travel expenses of $141,000, offset by decreased
consulting costs of $953,000, a decrease in other marketing costs of $230,000 and decreased subscription costs of $193,000. We expect
sales and marketing expenses to decrease in 2025 due to recent personnel changes.

 General
and Administrative Expenses. General and administrative expenses consist primarily of personnel-related expenses, professional
fees, including legal, finance and accounting expenses and other infrastructure expenses. General and administrative expenses for the
year ended December 31, 2024 decreased by $1,922,000, or 16%, compared to the same period in 2023. This
decrease was primarily due to a decrease in employment-related expenses of $422,000, a decrease in consulting costs of $519,000, a decrease
in outside legal costs of $247,000, decreased accounting costs of $204,000 and a decrease in public company expenses of $174,000. We
expect general and administrative expenses to decrease further in 2025 due to recent personnel changes.

 Change
in fair value of warrant liabilities. The fair values of the warrants as of December 31, 2024, and December 31, 2023 were
$60,000 and $1,651,000, respectively. This represents the change in fair value of warrants exercised of $245,000, as well as a net
change in fair value of $1,836,000, offset by an increase of $490,000 due to the modification of certain warrant
liabilities.

 Interest
Income (Expense), net. We had net interest expense of $33,000 and net interest income of $48,000, for the years ended December
31, 2024 and 2023, respectively. The change in the net interest expense was primarily related to a decrease in the interest earned on
our money market accounts, offset by the lower interest on the DECD loan after the forgiveness of $1,000,000.

 Forgiveness
of DECD loan. Forgiveness of the DECD loan decreased $1,000,000, compared to the same period in 2023. $1,000,000 of our loan
with the State of Connecticut Department of Economic and Community Development (the "DECD") was partially forgiven in 2023.

 Other
Income (Expense), net. Other income for the year ended December 31, 2024 increased by $1,386,000, compared to the same period
in 2023. The increase related primarily to one-time transactions, including an award received from
the federal government in the amount of $2,000,000. The increase was offset by the receipt of Employee Retention Tax Credits of
$347,000 and the receipt of insurance reimbursements of $250,000 in 2023.

 Cash
Flows The following table summarizes our cash flows for the periods ended December 31, 2024 and 2023.

 2024

 2023

 Net cash (used in) provided by:

 Operating activities

 $
 (12,113
)

 $
 (15,894
)

 Investing activities

 (37
)

 (24
)

 Financing activities

 11,064

 5,216

 Net decrease in cash, cash equivalents and restricted cash

 $
 (1,086
)

 $
 (10,702
)

 Net
cash used in operating activities was $12,113,000 for the year ended December 31, 2024, resulting primarily from the net loss reported
of $13,094,000 and changes in fair value of warrant liabilities in the amount of approximately $1,346,000 and $418,000 related to changes
in accrued liabilities, primarily offset by $1,494,000 related to non-cash stock compensation expense, $912,000 related to changes in
accounts payable and $469,000 related to changes in accounts receivable.

 Net
cash used in operating activities was $15,894,000 for the year ended December 31, 2023, resulting primarily from the net loss reported
of $16,690,000, the forgiveness of our DECD loan of $1,000,000 and changes in fair value of warrant liabilities in the amount
of approximately $629,000, primarily offset by $1,724,000 related to non-cash stock compensation expense and $577,000 related to changes
in prepaid expenses and other assets.

 Net
cash used in investing activities was $37,000 and $24,000 for the years ended December 31, 2024 and 2023, respectively, which consisted
primarily of property and equipment purchases.

 Net
cash provided by financing activities was $11,064,000 for the year ended December 31, 2024, related
primarily to a registered direct offering resulting in net proceeds of $4,830,000, after deducting placement agent costs and other
expenses of $733,000, net proceeds of $1,901,000 related to an equity line of credit agreement, net proceeds of $1,838,000 related
to a private placement offering, after deducting placement agent costs and other expenses of $72,000, net proceeds of $1,862,000
related to a warrant inducement agreement, after deducting placement agent costs and other expenses of $277,000 and net proceeds of
$715,000 related to an at the market offering, after deducting transaction-related offering costs of $189,000, partially offset by
principal payments on the DECD loan of $93,000.

 Net
cash provided by financing activities was $5,216,000 for the year ended December 31, 2023, related
primarily to a registered direct offering resulting in net proceeds of $4,119,000, after deducting placement agent costs and other
expenses of $597,000, net proceeds of $68,000 related to an at the market offering, after deducting transaction-related
offering costs of $134,000, and an equity line of credit offering of $1,177,000, partially offset by principal payments on the DECD
loan of $148,000.

 We
have significant NOL carryforwards as of December 31, 2024 which are subject to a full valuation allowance due to our history of operating
losses. Section 382 of the Internal Revenue Code of 1986, as amended ("Section 382"), as well as similar state provisions
restrict our ability to use our NOL credit carryforwards to offset taxable income due to ownership change limitations that have occurred
in the past or that could occur in the future. These ownership changes also may limit the amount of tax credit carryforwards that can
be utilized annually to offset tax liabilities.

 Our
pre- 2018 federal NOLs will expire in varying amounts from 2025 through 2037, if not utilized and can offset 100% of future taxable
income for regular tax purposes. Any federal NOLs arising on or after January 1, 2018, can be carried forward indefinitely but such federal
NOL carryforwards are permitted to be used in any taxable year to offset up to 80% of taxable income in such year. Portions of our state
NOLs will expire in varying amounts from 2025 through 2044 if not utilized. Our ability to use our NOLs will be dependent on our ability
to generate taxable income, and the portions of our NOLs could expire before we generate sufficient taxable income.

 Our
ability to use our NOL carryforwards to offset taxable income is restricted due to ownership change limitations that have occurred in
the past or that could occur in the future, as required by Section 382, as well as similar state specific provisions.

 Our
management believes that Section 382 ownership changes most recently occurred as a result of our follow-on public offerings in 2011 and
2013.

 These
limitations may result in the expiration of a portion of our NOL carryforwards before utilization. Due to the existence of a full valuation
allowance against our remaining NOLs, it is not expected that Section 382 limitations will have an impact on our results of operations
or financial position.

 Liquidity
and Capital Resources

 We
plan to continue to expend resources selling and marketing or ovarian cancer and endometriosis offerings and developing additional diagnostic
tests and service capabilities.

 We
do not believe our existing cash and cash equivalents balance and cash flow from operations will be sufficient to meet our working capital,
capital expenditures, and material cash requirements from known contractual obligations for the next twelve months and beyond. Our future
capital requirements, the adequacy of available funds, and cash flows from operations could be affected by various risks and uncertainties,
including, but not limited to, those detailed in Part I, Item 1A, Risk Factors in this Annual Report. We have incurred significant net
losses and negative cash flows from operations since inception, and as a result has an accumulated deficit of approximately $531,397,000
as of December 31, 2024. We also expect to incur a net loss and negative cash flows from operations for 2025. In order to continue our
operations as currently planned through 2025 and beyond, we will need to raise additional capital, which may include public or private
equity offerings, debt financing, collaborations, licensing arrangements. Given the above conditions, there is substantial doubt about
our ability to continue as a going concern. The consolidated financial statements have been prepared on a going concern basis and do
not include any adjustments that might result from these uncertainties.

 Contractual
Obligations

 Loan
Agreement

 In
March 2016, we entered into a loan agreement (as amended on March 7, 2018 and April 3, 2020, the "DECD Loan Agreement") with
the State of Connecticut Department of Economic and Community Development (the "DECD"), pursuant to which we may borrow up
to $4,000,000 from the DECD.

 The
loan may be prepaid at any time without premium or penalty. We received an initial disbursement of $2,000,000 on April 15, 2016 under
the DECD Loan Agreement. As we had achieved the target employment milestone necessary to receive an additional $1,000,000 under the DECD
Loan Agreement and the DECD determined to fund the remaining $1,000,000 under the DECD Loan Agreement after concluding that the required
revenue target would likely have been achieved in the first quarter of 2020 in the absence of the impacts of COVID-19, on December 3,
2020, we received a disbursement of the remaining $2,000,000 available under the DECD Loan Agreement.

 Under
the terms of the DECD Loan Agreement, we were eligible for forgiveness of up to $1,500,000 of the principal amount of the loan had we
achieved certain job creation and retention milestones by December 31, 2022. On June 26, 2023, we were notified by the DECD that we had
satisfied all job creation and retention requirements under the loan agreement to receive forgiveness of $1,000,000. If we fail to maintain
our Connecticut operations through March 22, 2026, the DECD may require early repayment of a portion or all of the loan plus a penalty
of 5% of the total funded loan. For additional information, see Note 6 of our consolidated financial statements. As of December 31, 2024,
the remaining balance outstanding under the DECD Loan Agreement is approximately $1,511,000, net of issuance costs.

 Operating
Leases

 As
of December 31, 2024, we are engaged in two lease agreements. Our Austin, Texas lease renewal agreement has a term of 81 months and expires
on August 31, 2031, with the option to extend the lease for an additional three years. Our Shelton, Connecticut lease renewal agreement
has a five-year term and expires on September 30, 2028, with a five-year renewal option.

 Non-cancelable
Royalty Obligations and Other Commitments

 We
are a party to an amended research collaboration agreement with The Johns Hopkins University School of Medicine under which we license
certain of its intellectual property directed at the discovery and validation of biomarkers in human subjects, including but not limited
to clinical application of biomarkers in the understanding, diagnosis and management of human disease. Under the terms of the amended
research collaboration agreement, Aspira is required to pay the greater of 4% royalties on net sales of diagnostic tests using the assigned
patents or annual minimum royalties of $57,500. Royalty expense for the years ended December 31, 2024 and 2023 totaled $293,000 and $324,000,
respectively, as recorded in cost of revenue in the consolidated statements of operations.

 Business
Agreements

 In
August 2022, we entered into a sponsored research agreement with Harvard's Dana-Farber Cancer Institute, Brigham & Women's
Hospital, and Medical University of Lodz for the generation of a multi-omic, non-invasive diagnostic aid to identify endometriosis based
on circulating miRNAs and proteins. The results of this collaboration will be advanced, co-developed technology to guide medical and
clinical management of women presenting with symptoms of endometriosis. This collaboration is expected to accelerate the development
and commercialization of future endometriosis products, such as ENDOinform. The contract requires payments to be made upon the achievement
of certain milestones. Under the terms of and as further described in the agreement, payments of approximately $1,252,000 are due from
us to the counterparties upon successful completion of certain deliverables. During the year ended December 31, 2024, approximately $118,000
has been recorded as research and development expense in our consolidated financial statement of operations for the project. During the
year ended December 31, 2023, approximately $215,000, was recorded as research and development expense in our consolidated financial
statement of operations for the project. From the inception of the Dana-Faber, Brigham, Lodz Research Agreement through December 31,
2024, research and development expenses in the cumulative amount of $1,202,000 have been recorded. From the inception of the Dana-Faber,
Brigham, Lodz Research Agreement through December 31, 2024, we made payments totaling $1,040,000. Additional payments of $212,000 are
due to the collaboration partners in 2025 upon completion of certain deliverables estimated to occur during 2025.

 On
March 20, 2023, we entered into a licensing agreement with Harvard's Dana-Farber Cancer Institute, Brigham & Women's
Hospital, and Medical University of Lodz (the "Ovarian Cancer License Agreement") under which the Company will license certain
of its intellectual property to be used in our OvaSuite product portfolio. Under the terms of the Ovarian Cancer License Agreement, we
paid an initial license fee of $75,000 and then will pay a license maintenance fee of $50,000 on each anniversary of the date, as well
as non-refundable royalty payments of up to $1,350,000 based on certain regulatory approvals and commercialization milestones and further
royalty payments based on the net sales of our products included. No milestones have been reached as of December 31, 2024, and no royalty
payments have been paid to date.

 Common
Stock

 On
February 10, 2023, we entered into a Controlled Equity Offering Sales Agreement (the "Cantor Sales Agreement"), with Cantor
Fitzgerald & Co. ("Cantor") as agent, pursuant to which it could offer and sell, from time to time, through Cantor, shares
of our common stock, par value $0.001 per share, having an aggregate offering price of up to $12.5 million (the "Placement Shares").

 Under the Cantor Sales Agreement,
Cantor could sell the Placement Shares by any method permitted by law and deemed to be an "at the market offering" as defined
in Rule 415 promulgated under the Securities Act of 1933, as amended, or the Securities Act, including sales made directly on the Nasdaq
Capital Market, on any other existing trading market for our common stock or to or through a market maker or in privately negotiated transactions.
Cantor received a Placement Fee of 3% for each completed sale of Placement Shares under the Cantor Sales Agreement.

 We were not obligated to make any
sales of the Placement Shares under the Cantor Sales Agreement.

the year ended December 31, 2023, we sold 35,552 shares of the Placement Shares,
for gross proceeds of approximately $211,000. For the year ended December 31, 2023, we recorded $134,000 as an offset to additional paid-in
capital representing transaction-related offering costs of the Placement Shares.

 In connection with a follow-on
equity offering on July 24, 2023, we delivered written notice to Cantor on July 19, 2023 that we were suspending the prospectus supplement,
dated February 10, 2023, related to our common stock issuable under the Cantor Sales Agreement. The 2023 At the Market Agreement was terminated
in August 2024.

 On
March 28, 2023, we entered into a purchase agreement (the "2023 Equity Line of Credit Agreement") with Lincoln Park Capital
Fund, LLC ("Lincoln Park") and a registration rights agreement (the "LPC Registration Rights Agreement"), pursuant
to which we have the right, in our sole discretion, to sell to Lincoln Park shares of our common stock, par value $0.001 per share (the
"Common Stock"), having an aggregate value of up to $10,000,000 (the "Purchase Shares"), subject to certain limitations
and conditions set forth in the 2023 Equity Line of Credit Agreement. We control the timing and amount of any sales of Purchase Shares
to Lincoln Park pursuant to the 2023 Equity Line of Credit Agreement.

 Under
the 2023 Equity Line of Credit Agreement, on any business day after March 28, 2023 selected by us over the 36-month term of the 2023
Equity Line of Credit Agreement (each, a "Purchase Date"), we may direct Lincoln Park to purchase up to 6,667 shares of Common
Stock on such Purchase Date (a "Regular Purchase"); provided, however, that (i) a Regular Purchase may be increased to up
to 13,333 shares, if the closing sale price per share of the Common Stock on The Nasdaq Capital Market is not below $7.50 on the applicable
Purchase Date; (ii) a Regular Purchase may be increased to up to 16,666 shares, if the closing sale price per share of the Common Stock
on The Nasdaq Capital Market is not below $11.25 on the applicable Purchase Date; and (iii) a Regular Purchase may be increased to up
to 20,000 shares, if the closing sale price per share of the Common Stock on The Nasdaq Capital Market is not below $15.00 on the applicable
Purchase Date. In any case, Lincoln Park's maximum obligation under any single Regular Purchase will not exceed $1,000,000. The
above-referenced share amount limitations and closing sale price thresholds are subject to adjustment for any reorganization, recapitalization,
non-cash dividend, stock split, reverse stock split or other similar transaction as provided in the 2023 Equity Line of Credit Agreement.
The purchase price per share for each such Regular Purchase will be equal to the lesser of:

 1.

 the
 lowest sale price for the Common Stock on The Nasdaq Capital Market on the date of sale; and

 2.

 the
 average of the three lowest closing sale prices for the Common Stock on The Nasdaq Capital Market during the 10 consecutive business
 days ending on the business day immediately preceding the purchase date.

 We
also have the right to direct Lincoln Park, on any business day on which we have properly submitted a Regular Purchase notice for the
maximum amount we are then permitted to sell to Lincoln Park in such Regular Purchase, to purchase an additional amount of the Common
Stock (an "Accelerated Purchase") of additional shares based on criteria established in the 2023 Equity Line of Credit Agreement.
An Accelerated Purchase, which is at our sole discretion, may be subject to additional requirements and discounts if certain conditions
are met as defined in the 2023 Equity Line of Credit Agreement.

 The
issuance of the Purchase Shares had been previously registered pursuant to the our effective shelf registration statement on Form S-3
(File No. 333-252267) (the "Old Registration Statement"), and the related base prospectus included in the Registration Statement,
as supplemented by a prospectus supplement filed on March 28, 2023, that has expired. On April 22, 2024, we filed a registration statement
on Form S-3 (File No. 333-278867) (the "Registration Statement"), and the related base prospectus included in the Registration
Statement, that was declared effective by the SEC on April 25, 2024.

the year ended December 31, 2023, we sold 472,312 shares of Common Stock under the 2023 Equity Line of Credit Agreement for gross
proceeds of approximately $1,578,000 under the Old Registration Statement. In addition, 47,733 shares of Common Stock were issued to
Lincoln Park as consideration for entering into the 2023 Equity Line of Credit Agreement.

the year ended December 31, 2024, we sold 949,574 shares under the 2023 Equity Line of Credit Agreement for gross proceeds of approximately
$1,900,000. Over the life of the 2023 Equity Line of Credit Agreement through December 31, 2024, we sold 1,310,517 shares for gross proceeds
of approximately $3,078,000. We incurred approximately $326,000 of costs related to the execution of the 2023 Equity Line of Credit Agreement,
all of which are reflected in the unaudited condensed consolidated financial statements. Of the total costs incurred, approximately $258,000
was paid in common stock to Lincoln Park for a commitment fee and $30,000 was paid for Lincoln Park expenses. These transaction costs
were included in other expense in our consolidated statement of operations for the year ended December 31, 2023. We incurred approximately
$249,000 and $41,000 for legal fees during the year ended December 31, 2024 and 2023, respectively, and included the costs in general
and administrative expenses on its consolidated statement of operations. Under the terms of the Warrant Inducement Agreement, we agreed
not to sell shares under the 2023 Equity Line of Credit Agreement for six months from the effective date of the Form S-3, which was September
3, 2024. As of March 25, 2025, the remaining availability under the 2023 Equity Line of Credit Agreement was $1,700,000 of shares of
Common Stock that can be sold to Lincoln Park under the 2023 Equity Line of Credit Agreement, subject to the terms of the 2023 Equity
Line of Credit Agreement.

 On
July 20, 2023, we entered into a securities purchase agreement (the "2023 Direct Offering Agreement"), with several investors
relating to the issuance and sale of 1,694,820 shares of our common stock, par value $0.001 per share (the "2023 Direct Offering").

to the 2023 Direct Offering Agreement, we issued 1,650,473 shares of common stock to certain investors at an offering price of $2.75
per share, and 44,347 shares of common stock to its directors and executive officers at an offering price of $3.98 per share, which was
the consolidated closing bid price of our common stock on The Nasdaq Capital Market on July 19, 2023. Our aggregate gross proceeds from
the 2023 Direct Offering were approximately $4.7 million, before deducting placement agent fees and other estimated expenses of $597,000
payable by us.

 We engaged Alliance Global Partners ("AGP")
to act as sole placement agent in the 2023 Direct Offering. We paid the placement agent a cash fee equal to 7.0% of the aggregate gross
proceeds generated from the 2023 Direct Offering, except that, with respect to proceeds from the sale of 182,447 shares of common stock
to certain investors, including our directors and executive officers, the placement agent's cash fee was 3.5%. We also reimbursed
the placement agent for its accountable offering-related legal expenses of $75,000 and a non-accountable expense allowance of $30,000.

 On
January 24, 2024, we entered into a securities purchase agreement (the "2024 Direct Offering Agreement"), with several investors
relating to the issuance and sale of 1,371,000 shares of our common stock, par value $0.001 per share, and pre-funded warrants to purchase
200,000 shares of Common Stock (the "Pre-Funded Warrants"), in a registered direct offering, together with accompanying warrants
to purchase 1,571,000 shares of Common Stock (the "Purchase Warrants", and together with the Pre-Funded Warrants, the "Warrants")
in a concurrent private placement (the "Concurrent Private Offering" and together with the registered direct offering, the
"2024 Direct Offering").

to the 2024 Direct Offering Agreement, we issued 1,368,600 shares of common stock to certain investors at an offering price of $3.50
per share, and 2,400 shares of common stock to an executive officer, at an offering price of $4.255 per share, which was the consolidated
closing bid price of our common stock on The Nasdaq Capital Market on January 24, 2024 of $4.13 per share plus $0.125 per Purchase Warrant.
The purchase price of each Pre-Funded Warrant is equal to the combined purchase price at which a share of Common Stock and the accompanying
Purchase Warrant is sold in this 2024 Direct Offering, minus $0.0001. Our gross proceeds from the 2024 Direct Offering were approximately
$5,563,000, before deducting placement agent fees and other expenses of approximately $733,000 payable by us. The 2024 Direct Offering
closed on January 26, 2024.

 All of the Pre-Funded Warrants were exercised on February 6, 2024 for gross proceeds of $20.

 The
Purchase Warrants have an exercise price of $4.13 per share and were exercisable beginning six months after issuance. 1,400,000 of the
Purchase Warrants were exercised on August 1, 2024 under the Warrant Inducement Agreement at a reduced price of $1.25 per share.

 We
engaged AGP to act as sole placement agent in the 2024 Direct Offering. We paid the placement agent a cash fee equal to 7.0% of the aggregate
gross proceeds generated from the 2024 Direct Offering, except that, with respect to proceeds raised in this 2024 Direct Offering from
certain designated persons, AGP's cash fee is reduced to 3.5% of such proceeds, and to reimburse certain fees and expenses of the
placement agent in connection with the 2024 Direct Offering. We also reimbursed the placement agent for its accountable offering-related
legal expenses of $75,000 and a non-accountable expense allowance of $30,000. Costs related to the 2024 Direct Offering were recorded
as an offset to additional paid-in capital on our balance sheet as of December 31, 2024.

 Effective
upon the closing of the 2024 Direct Offering, we also amended certain existing warrants (the "August 2022 Warrants") to purchase
up to an aggregate of 366,664 shares at an exercise price of $13.20 per share and a termination date of August 25, 2027, so that the
amended August 2022 Warrants have a reduced exercise price of $4.13 per share and a new termination date of January 26, 2029. The other
terms of the amended August 2022 Warrants remain unchanged. We performed an analysis of the fair value of the August 2022 Warrants
immediately before and after the modification and the increase in fair value of the August 2022 Warrants of $490,000 was recorded as
a change in fair value of warrant liabilities in our consolidated statement of operations.

 Approximately
$106,000 of the costs related to the 2024 Direct Offering were allocated to the August 2022 Warrants and were recorded as other expense
in our consolidated statement of operations.

 On
July 1, 2024, we entered into a securities purchase agreement with certain investors in a private placement (the "2024 Private
Placement Offering"). Pursuant to the 2024 Private Placement Offering, we issued an aggregate of 1,248,529 shares of our common
stock and accompanying warrants (the "July 2024 Warrants") to purchase an equal number of shares of common stock at a price
of $1.53 per share and accompanying warrant. The July 2024 Warrants have an exercise price of $2.25 per share and are exercisable until
their expiration on the third anniversary of the issuance date. Our gross proceeds from the 2024 Private Placement Offering were approximately
$1,909,000, before deducting expenses of approximately $72,000 payable by us.

 In
February 2025, certain July 2024 Warrants were modified to require shareholder approval of the July 2024 Warrants prior to their becoming
exercisable.

 On
August 2, 2024, we entered into an agreement with H.C. Wainwright in connection with an At the Market offering agreement (the "2024
At the Market Offering") to sell shares of our common stock ("Common Stock"), having an aggregate sales price of up
to $4,450,000, from time to time, through an "at the market offering" program under which H.C. Wainwright acts as sales agent.
We pay Wainwright a commission rate equal to 3.0% of the aggregate gross proceeds from each sale of shares under the 2024 At the Market
Offering. We have also reimbursed H.C. Wainwright for certain specified expenses in connection with entering into the 2024 At the Market
Offering.

the year ended December 31, 2024, we sold 1,073,050 shares under the 2024 At the Market Offering for gross proceeds of approximately
$903,000. We incurred approximately $240,000 of costs related to the execution of the 2024 At the Market Offering, all of which were
recorded as an offset to additional paid-in capital on our balance sheet as of December 31, 2024.

 Subsequent
to December 31, 2024 and through March 25, 2025, we sold 12,277,441 shares under the 2024 At the Market Offering Agreement for gross
proceeds of approximately $3,483,000 before deducting expenses of approximately $146,000. As of March 25, 2025, the remaining availability
under the 2024 At the Market Offering Agreement was approximately $62,000 of shares of Common Stock that can be sold to H.C. Wainwright
under the 2024 At the Market Offering Agreement, subject to the terms of the 2024 At the Market Offering Agreement.

 In
August 2024, we entered into securities purchase agreements with two shareholders under which we sold a total of 9,733 shares of common
stock and received proceeds of approximately $11,000.

 On
July 31, 2024, we entered into a warrant inducement agreement (the "Warrant Inducement Agreement") with a certain holder
(the "Holder") of (i) warrants to purchase 311,111 shares of Common Stock dated August 22, 2022 (the "August 2022 Warrants")
and (ii) warrants to purchase 1,400,000 shares of Common Stock dated January 26, 2024 (the "January 2024 Warrants"), pursuant
to which the Holder agreed to exercise in cash the warrants held at a reduced exercise price of $1.25 per share (reduced from $4.13 per
share for the August 2022 Warrants and $4.13 for the January 2024 Warrants).

 As
an inducement to such exercise, we agreed to issue to the Holder new Common Stock warrants (collectively, the "August 2024 Warrants"),
to purchase up to 2,566,667 shares of Common Stock. The August 2024 Warrants were exercisable immediately after issuance and will expire
5 years from the initial exercise date.

 The
transaction, which closed on August 1, 2024, resulted in net proceeds of approximately $1,862,000. The Warrant Inducement Agreement was
entered into to encourage the exercise of the August 2022 Warrants and January 2024 Warrants in order to obtain capital for operations.
The $1,323,000 incremental value transferred for the modification to both the August 2022 Warrants and January 2024 Warrants as a result
of the Warrant Inducement Amendment was accounted for as an equity issuance cost and recognized within additional paid in capital in
the audited consolidated balance sheets.

 In
connection with a private placement offering of common stock and warrants we completed in May 2013, we entered into the 2013 Stockholders
Agreement which, among other things, gives two of the primary investors in that offering the right to participate in any of our future
equity offerings on the same price and terms as other investors. In addition, the 2013 Stockholders Agreement prohibits us from taking
certain material actions without the requisite consent. These material actions include:

 Making
 any acquisition with a value greater than $2 million;

 Offering,
 selling or issuing any securities senior to our common stock or any securities that are convertible into or exchangeable or exercisable
 for securities ranking senior to our common stock;

 Taking
 any action that would result in a change in control of the Company or an insolvency event; and

 Paying
 or declaring dividends on any of our securities or distributing any of our assets other than in the ordinary course of business or
 repurchasing any of our outstanding securities.

 The
foregoing rights terminate for a primary investor when that investor ceases to beneficially own less than 50% of the shares and warrants
(taking into account shares issued upon exercise of the warrants), in the aggregate, that were purchased at the closing of the 2013 private
placement. We believe that the rights of one of the primary investors have so terminated.

 We
have incurred significant net losses and negative cash flows from operations since inception. At December 31, 2024 we had an accumulated
deficit of $531,397,000 and stockholders' deficit of $2,563,000. As of December 31, 2024, we had $1,769,000 of cash and cash equivalents,
and $5,468,000 of current liabilities. Our working capital deficit was $1,285,000 at December 31, 2024. There can be no assurance that
we will achieve or sustain profitability or positive cash flow from operations. In addition, while we expect to grow revenue through
Aspira Labs, there is no assurance of our ability to generate substantial revenues and cash flows from Aspira Labs' operations.
We expect revenue from our products to be our only material, recurring source of cash in 2025.

 We
expect to incur a net loss and negative cash flows from operations in 2025.

 Our
future liquidity and capital requirements will depend upon many factors, including, among others:

 resources
 devoted to sales, marketing and distribution capabilities;

 the
 rate of product adoption by physicians and patients;

 the
 rate of product adoption by healthcare systems and large physician practices of the decentralized distribution agreements;

 the
 insurance payer community's acceptance of and reimbursement for our products;

 our
 plans to acquire or invest in other products, technologies and businesses; and

 the
 potential need to add study sites to access additional patients to maintain clinical timelines;

 In
the event that our existing cash on hand is not sufficient to fund our near or long term operations, meet our capital requirements or
satisfy our anticipated obligations as they become due, we expect to take further action to protect our liquidity position. Such actions
may include, but are not limited to:

 raising
 capital through an equity offering either in the public markets or via a private placement offering (however, no assurance can be
 given that capital will be available on acceptable terms, or at all);

 reducing
 executive bonuses or replacing cash compensation with equity grants;

 reducing
 professional services and consulting fees and eliminating non-critical projects;

 reducing
 travel and entertainment expenses; and

 reducing,
 eliminating or deferring discretionary marketing programs.

 ITEM
 7A.

 QUANTITATIVE
 AND QUALITATIVE DISCLOSURES ABOUT MARKET RISK

to Item 305(e) of Regulation S-K, the information called for by Item 7A is not required.

 ITEM
 8.

 CONSOLIDATED
 FINANCIAL STATEMENTS AND SUPPLEMENTARY DATA

 Our
consolidated financial statements, including consolidated balance sheets as of December 31, 2024 and 2023, consolidated statements of
operations for the years ended December 31, 2024 and 2023, consolidated statements of changes in stockholders' equity for the years
ended December 31, 2024 and 2023, consolidated statements of cash flows for the years ended December 31, 2024 and 2023 and notes to our
consolidated financial statements, together with a report thereon of our independent registered public accounting firm are attached hereto
as pages F-1 through F-29.

 ITEM
 9.

 CHANGES
 IN AND DISAGREEMENTS WITH ACCOUNTANTS ON ACCOUNTING AND FINANCIAL DISCLOSURE

 None.

 ITEM
 9A.

 CONTROLS
 AND PROCEDURES

 Evaluation
of Disclosure Controls and Procedures

 Our
senior management is responsible for establishing and maintaining a system of disclosure controls and procedures (as defined in Rule
13a-15(e) and 15d-15(e) under the Securities Exchange Act of 1934, as amended (the "Exchange Act") designed to ensure that
information required to be disclosed by us in the reports that we file or submit under the Exchange Act is recorded, processed, summarized
and reported within the time periods specified in the SEC's rules and forms. Disclosure controls and procedures include, without
limitation, controls and procedures designed to ensure that information required to be disclosed by an issuer in the reports that it
files or submits under the Exchange Act is accumulated and communicated to the issuer's management, including its principal executive
officer and principal financial officer, or persons performing similar functions, as appropriate to allow timely decisions regarding
required disclosure.

 Management,
including our Chief Executive Officer and Vice President of Finance, performed an evaluation of our disclosure controls and procedures
(as defined in Rules 13a-15(e) and 15d-15(e) under the Exchange Act) as of December 31, 2024. Based on this evaluation, our Chief
Executive Officer and Vice President of Finance have concluded that as of December 31, 2024, our disclosure controls and procedures
were not effective.

 Management's
Annual Report on Internal Control over Financial Reporting

 We
are responsible for establishing and maintaining adequate internal control over our financial reporting, as such term is defined in Exchange
Act Rules 13a-15(f) and 15d-15(f). Our management has assessed the effectiveness of internal control over financial reporting as of December
31, 2024. Our assessment was based on criteria set forth by the Committee of Sponsoring Organizations of the Treadway Commission ("COSO")
entitled "Internal Control - Integrated Framework (2013)."

 Our
internal control over financial reporting is a process designed to provide reasonable assurance regarding the reliability of financial
reporting and the preparation of financial statements for external purposes in accordance with GAAP. Our internal control over financial
reporting includes those policies and procedures that:

 (i)

 pertain
 to the maintenance of records that, in reasonable detail, accurately and fairly reflect our transactions and dispositions of our
 assets;

 (ii)

 provide
 reasonable assurance that transactions are recorded as necessary to permit preparation of financial statements in accordance with
 GAAP, and that our receipts and expenditures are being made only in accordance with authorizations of our management and board of
 directors; and

 (iii)

 provide
 reasonable assurance regarding prevention or timely detection of unauthorized acquisition, use, or disposition of our assets that
 could have a material effect on the financial statements.

 Because
of its inherent limitations, internal control over financial reporting may not prevent or detect misstatements. Also, projections of
any evaluation of effectiveness to future periods are subject to the risk that controls may become inadequate because of changes in conditions,
or that the degree of compliance with the policies or procedures may deteriorate.

 Based on using the COSO criteria, management concluded our internal
control over financial reporting as of December 31, 2024 was not effective.

 This Form 10-K does not include
an attestation report of our independent registered public accounting firm regarding internal control over financial reporting. Management's
assessment of the effectiveness of our internal control over financial reporting as of December 31, 2024 was not subject to attestation
by our independent registered public accounting firm pursuant to rules of the SEC that permit a smaller reporting company to provide only
management's report in our Annual Report on Form 10-K.

the year ended December 31, 2024, we identified a material weakness in internal control over financial reporting related to the
operation of internal controls related to our contract review processes and the accounting for such contracts. This material
weakness related to the accounting for complex financial transactions, including the technical accounting conclusions reached.
During the year ended December 31, 2024, we entered into two transactions that were considered to be significant, non-routine or
complex transactions, including a warrant inducement and a government grant. While the control was adequately designed, the operation of the control was not
effective for either transaction.

 The aggregation of these two deficiencies
resulted in material weaknesses in our internal control over financial reporting as of December 31, 2024. A material weakness is a deficiency,
or a combination of deficiencies, in internal control over financial reporting, such that there is a reasonable possibility that a material
misstatement of our annual or interim financial statements will not be prevented or detected on a timely basis. As a result, our management
concluded that as of December 31, 2024, our internal control over financial reporting was not effective.

 Remediation Activities

 In
order to address the material weaknesses in internal control over financial reporting as of December 31, 2024, described above,
management implemented remediation activities, with direction from the audit committee. The activities that we have taken include
retaining outside accounting assistance from a nationally recognized firm for certain significant, non-routine or complex
transactions, including warrant valuation, beginning in the first quarter of 2025.

 Limitations
on Effectiveness of Controls and Procedures and Internal Control over Financial Reporting

 In
designing and evaluating the disclosure controls and procedures and internal control over financial reporting, management recognizes
that any controls and procedures, no matter how well designed and operated, can provide only reasonable assurance of achieving the desired
control objectives. In addition, the design of disclosure controls and procedures and internal control over financial reporting must
reflect the fact that there are resource constraints, and that management is required to apply judgment in evaluating the benefits of
possible controls and procedures relative to their costs.

 Remediation of Previously Identified Material
Weaknesses

 Management previously identified material weaknesses
in our internal control over financial reporting as of December 31, 2023 related to:

 1)

 Information technology general controls ("ITGCs") that are used to process and record certain revenue and expense transactions and support our financial reporting processes. This resulted in the lack of certain internal controls over these IT systems and over data and reports accumulated in such IT systems

 2)

 The design and implementation of our control activities over our revenue process. We did not adequately design controls to validate the delivery of the lab results to ordering physicians to ensure that revenue is being appropriately recognized.

 As of December 31, 2024, management implemented the
following to address the previously identified material weaknesses related to ITCGs and our revenue process:

 Retained
 an internal controls specialist to complement the skills of the existing accounting and financial reporting staff, as well as implement
 key controls to improve business processes, including revenue and the IT environment.

 Identified
 all information technology applications that support our financial reporting processes and assessed the risk of misstatement associated
 with each.

 Performed
 a comprehensive review of the design and performance of internal controls related to information technology applications, including
 user access and program change controls.

 Enhanced
 controls that require the assessment of service organization controls prior to implementation and on an annual basis.

 Enhanced the design of and implemented controls around the rigor of the
review process, and retention of sufficient appropriate evidence over the revenue process.

 Management determined these controls were in place
and operating for a sufficient period of time as of December 31, 2024 and, therefore, the previously identified material weaknesses related
to ITCGs and our revenue process were remediated as of December 31, 2024.

 Limitations
on Effectiveness of Controls and Procedures and Internal Control over Financial Reporting

 In designing and evaluating the
disclosure controls and procedures and internal control over financial reporting, management recognizes that any controls and procedures,
no matter how well designed and operated, can provide only reasonable assurance of achieving the desired control objectives. In addition,
the design of disclosure controls and procedures and internal control over financial reporting must reflect the fact that there are resource
constraints, and that management is required to apply judgment in evaluating the benefits of possible controls and procedures relative
to their costs.

 Changes in Internal Control over Financial Reporting

 Other than as described above,
there were no changes in our internal control over financial reporting during the quarter ended December 31, 2024 that materially affected,
or are reasonably likely to materially affect, our internal control over financial reporting.

 ITEM
 9B.

 OTHER
 INFORMATION

 None.

 ITEM
 9C.

 DISCLOSURE
 REGARDING FOREIGN JURISDICTIONS THAT PREVENT INSPECTIONS

 Not
applicable.

 PART
III

 ITEM
 10.

 DIRECTORS,
 EXECUTIVE OFFICERS AND CORPORATE GOVERNANCE

 The
information regarding our directors, committees of our board of directors, our director nomination process, and our executive officers
appearing under the heading "Election of Directors," "Corporate Governance," "Management", "Security
Ownership of Certain Beneficial Owners and Management" and "Delinquent Section 16(a) Reports" of the Company's
proxy statement relating to our annual meeting of stockholders to be held in 2025 (the "2025 Proxy Statement") is incorporated
by reference.

 ITEM
 11.

 EXECUTIVE
 COMPENSATION

 The
information appearing under the headings "Board Compensation," and "Executive Officer Compensation," of the 2025
Proxy Statement is incorporated by reference.

 ITEM
 12.

 SECURITY
 OWNERSHIP OF CERTAIN BENEFICIAL OWNERS AND MANAGEMENT AND RELATED STOCKHOLDER MATTERS

 The
information appearing under the heading "Security Ownership of Certain Beneficial Owners and Management" of the 2024 Proxy
Statement is incorporated by reference.

 The
information required by Item 201(d) of Regulation S-K will be set forth in the section titled "Equity
Compensation Plan Information" in the 2025 Proxy Statement and is incorporated herein by reference.

 ITEM
 13.

 CERTAIN
 RELATIONSHIPS AND RELATED TRANSACTIONS, AND DIRECTOR INDEPENDENCE

 The
information appearing under the headings "Certain Relationships and Related Transactions" and "Corporate Governance"
of the 2025 Proxy Statement is incorporated by reference.

 ITEM
 14.

 PRINCIPAL
 ACCOUNTANT FEES AND SERVICES

 The
information appearing under the heading "Ratification of the Selection of the Independent Registered Public Accounting Firm"
of the 2025 Proxy Statement is incorporated by reference.

 PART
IV

 ITEM
 15.

 EXHIBITS,
 FINANCIAL STATEMENT SCHEDULES

 (a)

 LIST
 OF DOCUMENTS FILED AS PART OF THIS REPORT:

 1.

 Financial
 Statements

 The
financial statements and notes thereto, and the report of the independent registered public accounting firm thereon, are set forth on
pages F-1 through F-29.

 2.

 Financial
 Statement Schedules

 All
financial statement schedules have been omitted as the information is not required under the related instructions or is not applicable
or because the information required is already included in the financial statements or the notes to those financial statements.

 (b)

 EXHIBITS

 Exhibit

 Incorporated
 by Reference

 Filed

 Number

 Exhibit
 Description

 Form

 File
 No.

 Exhibit

 Filing
 Date

 Herewith

 3.1

 Fourth Amended and Restated Certificate of Incorporation of Aspira Women's Health Inc. dated January 22, 2010

 000-31617

 3.1

 25, 2010

 3.2

 Certificate of Amendment of Fourth Amended and Restated Certificate of Incorporation, effective June 19, 2014

 3.2

 14, 2014

 3.3

 Certificate of Amendment to Fourth Amended and Restated Certificate of Incorporation of Vermillion, Inc. dated June 11, 2020

 3.1

 June
 11, 2020

 3.4

 Certificate of Amendment to Fourth Amended and Restated Certificate of Incorporation of Aspira Women's Health Inc, dated February 7, 2023

 3.1

 February
 7, 2023

 3.5

 Certificate of Designations, Preferences and Rights of Series B Convertible Preferred Stock

 4.1

 April
 17, 2018

 3.6

 Amended and Restated Bylaws of Aspira Women's Health Inc., effective February 23, 2022

 3.1

 February
 28, 2022

 3.7

 Certificate of Amendment to the Fourth Amended and Restated Certificate of Incorporation of Aspira Women's Health Inc., as amended May 11, 2023

 3.1

 May
 11, 2023

 4.1

 Form of Aspira Women's Health Inc.'s (formerly Ciphergen Biosystems, Inc.) Common Stock Certificate

 S-1/A

 333-32812

 4.1

 24, 2000

 4.2

 Securities Purchase Agreement dated May 8, 2013, by and among Aspira Women's Health Inc. (formerly Vermillion, Inc.) and the purchasers identified therein

 10.1

 May
 14, 2013

 4.3

 Stockholders Agreement dated May 13, 2013, by and among Vermillion, Inc., Oracle Partners, LP, Oracle Ten Fund Master, LP, Jack W. Schuler and other purchasers named therein

 10.2

 May
 14, 2013

 4.4

 Amended and Restated Promissory Note #1 by Vermillion, Inc. in favor of the State of Connecticut, acting by and through the Department of Economic and Community Development, effective April 3, 2020

 4.4

 April
 7, 2020

 4.5

 Amended and Restated Promissory Note #2 by Vermillion, Inc. in favor of the State of Connecticut, acting by and through the Department of Economic and Community Development, effective April 3, 2020

 4.5

 April
 7, 2020

 4.6

 Form of Indenture

 S-3

 333-252267

 4.7

 20, 2021

 4.7

 Description of Aspira Women's Health Inc.'s Securities Pursuant to Section 12 of the Securities Exchange Act of 1934

 4.7

 April
 1, 2024

 4.8

 Form of Warrant 2022

 4.1

 24, 2022

 4.9

 Form of Warrant Amendment to Common Stock Purchase Warrant_2022

 4.3

 26, 2024

 4.10

 Form of Pre-Funded Warrant 2024

 4.1

 26, 2024

 4.11

 Form of Warrant 2024

 4.2

 26, 2024

 4.12

 Form of Common Warrant June 2024

 4.1

 July
 2, 2024

 4.13

 Form of Warrant July 2024

 4.1

 July
 31, 2024

 4.14

 Form of Warrant Amendment to Common Stock Purchase Warrant June 2024

 4.15

 Form of Senior Convertible Note

 4.1

 March 11, 2025

 4.16

 Form of Warrant

 4.2

 March 11, 2025

 4.17

 Form of Securities Purchase Agreement

 10.1

 March 11, 2025

 10.1

 Securities Purchase Agreement, dated July 20, 2023, by and between Aspira Women's Health Inc. and the purchasers identified therein

 10.1

 July
 24, 2023

 10.2

 Form of Aspira Women's Health Inc Stock Option Award Agreement #

 10.4

 10, 2022

 10.3

 Form of Aspira Women's Health Inc Restricted Stock Award Agreement #

 10.5

 10, 2022

 10.4

 Aspira Women's Health Inc. 2019 Stock Incentive Plan #

 10.3

 10, 2022

 10.5

 Form of Aspira Women's Health Inc. Stock Option Award Agreement (non-employee) #

 10.7

 10, 2022

 10.6

 Assistance Agreement by and between the State of Connecticut, acting by and through the Department of Economic and Community Development and Vermillion, Inc. effective March 22, 2016

 10.1

 May
 16, 2016

 10.7

 Patent Security Agreement by Vermillion, Inc. in favor of the State of Connecticut, acting by and through the Department of Economic and Community Development, effective March 22, 2016

 10.3

 May
 16, 2016

 10.8

 Security Agreement by Vermillion, Inc. in favor of the State of Connecticut, acting by and through the Department of Economic and Community Development, effective March 22, 2016

 10.4

 May
 16, 2016

 10.9

 First Amendment to the Assistance Agreement by and between the State of Connecticut, acting by and through the Department of Economic and Community Development and Vermillion, Inc. dated March 7, 2018

 10.21

 March
 13, 2018

 10.10

 Second Amendment to the Assistance Agreement by and between the State of Connecticut, acting by and through the Department of Economic and Community Development and Vermillion, Inc. dated April 3, 2020

 10.22

 April
 7, 2020

 10.11

 Consulting Agreement between Aspira Women's Health Inc. and Nicole Sandford effective December 16, 2024 #

 10.12

 License Agreement between Aspira Women's Health Inc. and Dana-Farber Cancer Institute, Inc. effective March 20, 2023

 10.28

 April 1, 2024

 10.13

 Form of Securities Purchase Agreement June 2024

 10.1

 July
 2, 2024

 10.14

 Form of Warrant Inducement Agreement June 2024

 10.1

 July
 31, 2024

 10.15

 At The Market Agreement between Aspira Women's Health Inc. and H. C. Wainwright & Co., LLC dated August 2, 2024

 000-31617

 1.1

 2, 2024

 19.1

 Aspira Women's Health Inc. Insider Trading Policy

 21.0

 Subsidiaries of Registrant

 21.0

 March
 31, 2022

 23.1

 Consent of BDO USA, P.C., Independent Registered Public Accounting Firm

 24.1

 Power of Attorney (included on signature page hereto)

 31.1

 Certification of the Chief Executive Officer Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

 31.2

 Certification of the Chief Financial Officer Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002

 32.1

 Certification of the Chief Executive Officer and Chief Financial Officer pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

 97.1

 Aspira Women's Health Incentive Compensation Recoupment Policy

 97.1

 March 29, 2024

 Interactive

 Cover

 Filed
 herewith

 #

 Management
 contract or compensatory plan or arrangement.

 Confidential
 treatment has been granted with respect to certain provisions of this agreement. Omitted portions have been filed separately with
 the SEC.

 ITEM
 16.

 FORM
 10-K SUMMARY

 None.

 ASPIRA
WOMEN'S HEALTH INC.

TO CONSOLIDATED FINANCIAL STATEMENTS

 Page
 No.

 Report of Independent Registered Public Accounting Firm (BDO USA, P.C.; Boston, Massachusetts; PCAOB ID 243)

 Consolidated Balance Sheets as of December 31, 2024 and 2023

 Consolidated Statements of Operations for the years ended December 31, 2024 and 2023

 Consolidated Statements of Changes in Stockholders' (Deficit) Equity for the years ended December 31, 2024 and 2023

 Consolidated Statements of Cash Flows for the years ended December 31, 2024 and 2023

 Notes to Consolidated Financial Statements

 Report
of Independent Registered Public Accounting Firm

 Shareholders and Board of Directors

 Aspira Women's Health Inc.

 Austin, TX

 Opinion
on the Consolidated Financial Statements

 We
have audited the accompanying consolidated balance sheets of Aspira Women's Health Inc. (the "Company") as of December
31, 2024 and 2023, the related consolidated statements of operations, changes in stockholders' equity (deficit), and cash flows
for each of the years then ended, and the related notes (collectively referred to as the "consolidated financial statements").
In our opinion, the consolidated financial statements present fairly, in all material respects, the financial position of the Company
at December 31, 2024 and 2023, and the results of its operations and its cash flows for the years then ended, in conformity with
accounting principles generally accepted in the United States of America.

 Going
Concern Uncertainty

 The
accompanying consolidated financial statements have been prepared assuming that the Company will continue as a going concern. As discussed
in Note 1 to the consolidated financial statements, the Company has suffered recurring losses from operations and expects to continue
to incur substantial losses in the future, which raise substantial doubt about its ability to continue as a going concern. Management's
plans in regard to these matters are also described in Note 1. The consolidated financial statements do not include any adjustments that
might result from the outcome of this uncertainty.

 Basis
for Opinion

 These
consolidated financial statements are the responsibility of the Company's management. Our responsibility is to express an opinion
on the Company's consolidated financial statements based on our audits. We are a public accounting firm registered with the Public
Company Accounting Oversight Board (United States) ("PCAOB") and are required to be independent with respect to the Company
in accordance with the U.S. federal securities laws and the applicable rules and regulations of the Securities and Exchange Commission
and the PCAOB.

 We
conducted our audits in accordance with the standards of the PCAOB. Those standards require that we plan and perform the audit to obtain
reasonable assurance about whether the consolidated financial statements are free of material misstatement, whether due to error or fraud.
The Company is not required to have, nor were we engaged to perform, an audit of its internal control over financial reporting. As part
of our audits we are required to obtain an understanding of internal control over financial reporting but not for the purpose of expressing
an opinion on the effectiveness of the Company's internal control over financial reporting. Accordingly, we express no such opinion.

 Our
audits included performing procedures to assess the risks of material misstatement of the consolidated financial statements, whether
due to error or fraud, and performing procedures that respond to those risks. Such procedures included examining, on a test basis, evidence
regarding the amounts and disclosures in the consolidated financial statements. Our audits also included evaluating the accounting principles
used and significant estimates made by management, as well as evaluating the overall presentation of the consolidated financial statements.
We believe that our audits provide a reasonable basis for our opinion.

 Critical Audit Matters

 The critical audit matters communicated below are
matters arising from the current period audit of the consolidated financial statements that were communicated or required to be communicated
to the audit committee and that: (1) relate to accounts or disclosures that are material to the consolidated financial statements and
(2) involved our especially challenging, subjective, or complex judgments. The communication of these critical audit matters does not
alter in any way our opinion on the consolidated financial statements, taken as a whole, and we are not, by communicating these critical
audit matters below, providing separate opinions on the critical audit matters or on the accounts or disclosures to which they relate.

 Revenue
Recognition – Determination of Transaction Price for Product Revenue

 As
described in Note 1 to the consolidated financial statements, the Company recognizes product revenue upon completion of the test and
delivery of results to the physician based on estimates of the amounts that will ultimately be realized. When determining the amount
of revenue to be recognized for a delivered test result, management applies judgment to determine the transaction price, which affects the amount of revenue
recognized. The Company's product revenue for the year ended December 31, 2024 was $9.2 million.

 We
identified the auditing of management's determination of the transaction price as a critical audit matter. Management's determination
of the transaction price considers certain inputs, such as payment history, including amount and timing of payment and payer coverage.
Auditing these inputs required challenging auditor judgment due to the nature and extent of audit effort required.

 The primary procedures we performed to address this critical audit matter
included:

 Assessing the reasonableness of management's
 inputs used to estimate the transaction price by testing on a sample basis the underlying data by:

 Inspecting
 the supporting documentation related to the determination of payer grouping, and

 Reviewing
 historical cash collections used in determining the transaction price.

 Recalculating the average unit price using
 the historical cash collections for the period for each payer group.

 Accounting for July 2024 Warrant Inducement Agreement

 As described in Note 7 to the consolidated financial
statements, the Company entered into a warrant inducement agreement with a certain holder of (i) warrants to purchase 311,111 shares of
Common Stock dated August 22, 2022 and (ii) warrants to purchase 1,400,000 shares of Common Stock dated January 26, 2024, pursuant to
which the holder agreed to exercise in cash the warrants held at a reduced exercise price of $1.25 per share reduced from $4.13 per share.

 We identified the assessment of the accounting treatment
of these warrants as a critical audit matter due to the complexity in assessing the warrant features, which requires management to make
significant judgments in the interpretation of the terms of the agreements and in the application of appropriate accounting guidance.
Auditing management's application of the appropriate accounting treatment required challenging and complex auditor judgment due
to the nature and extent of audit effort required, including the use of firm personnel with expertise in technical accounting.

 The primary procedures we performed to address this
critical audit matter included:

 Reviewing
 and evaluating (i) the terms of the agreement, (ii) the completeness and accuracy of the Company's technical accounting analysis,
 and (iii) the application of the relevant accounting guidance.

 Utilizing
 firm personnel with expertise in the relevant technical accounting to assist in evaluating (i) the relevant contract terms of the
 issuances in relation to the appropriate accounting guidance, and (ii) the appropriateness of conclusions reached by the Company.

 /s/
 BDO USA, P.C.

 We
have served as the Company's auditor since 2012.

 Boston,
Massachusetts

 March

 F- 1

 Consolidated
Balance Sheets

 (Amounts
in Thousands, Except Share and Par Value Amounts)

 2024

 2023

 Assets

 Current assets:

 Cash and cash equivalents

 $
 1,769

 $
 2,597

 Accounts receivable, net of reserves of $ 0 and $ 15, as of December 31, 2024 and December 31, 2023, respectively

 1,459

 Prepaid expenses and other current assets

 1,098

 Inventories

 Total current assets

 4,183

 5,280

 Property and equipment, net

 Right-of-use assets

 1,194

 Restricted cash

 -

 Other assets

 Total assets

 $
 5,491

 $
 6,262

 Liabilities and Stockholders' (Deficit) Equity

 Current liabilities:

 Accounts payable

 $
 2,173

 $
 1,261

 Accrued liabilities

 2,445

 2,863

 Current portion of long-term debt

 Short-term debt

 Current maturities of lease liabilities

 Total current liabilities

 5,468

 5,119

 Non-current liabilities:

 Long-term debt

 1,278

 1,430

 Non-current maturities of lease liabilities

 1,248

 Warrant liabilities

 1,651

 Total liabilities

 8,054

 8,627

 Commitments and contingencies (Note 6)

 Stockholders' (deficit) equity:

 Common stock, par value $ 0.001 per share, 200,000,000 and 200,000,000 shares authorized at December 31, 2024 and December 31, 2023, respectively; 17,407,120 and 10,645,049 shares issued and outstanding at December 31, 2024 and December 31, 2023, respectively

 Additional paid-in capital

 528,817

 515,927

 Accumulated deficit

 ( 531,397
)

 ( 518,303
)

 Total stockholders' deficit

 ( 2,563
)

 ( 2,365
)

 Total liabilities and stockholders' deficit

 $
 5,491

 $
 6,262

 See
accompanying notes to consolidated financial statements

 F- 2

 Consolidated
Statements of Operations

 (Amounts
in Thousands, Except Share and Per Share Amounts)

 2024

 2023

 2024

 2023

 Revenue:

 $
 9,182

 $
 9,153

 Genetics

 -

 Total revenue

 9,182

 9,154

 Cost of revenue:

 3,703

 3,892

 Genetics

 -

 -

 Total cost of revenue

 3,703

 3,892

 Gross profit

 5,479

 5,262

 Operating expenses:

 3,266

 4,035

 8,146

 7,812

 10,345

 12,267

 Total operating expenses

 21,757

 24,114

 Loss from operations

 ( 16,278
)

 ( 18,852
)

 Other income, net:

 Change in fair value of warrant liabilities

 1,346

 Interest income (expense), net

 ( 33
)

 Forgiveness of DECD loan

 -

 1,000

 Other income, net

 1,871

 Total other income, net

 3,184

 2,162

 $
)

 $
)

 Net loss per share - basic and diluted

 $
 ( 0.93
)

 $
 ( 1.81
)

 Weighted average common shares used to compute basic and diluted net loss per common share

 14,134,626

 9,233,306

 See
accompanying notes to consolidated financial statements

 F- 3

 Consolidated
Statements of Changes in Stockholders' (Deficit) Equity

 (Amounts
in Thousands, Except Share Amounts)

 Shares

 Amount

 Capital

 Deficit

 (Deficit)

 Common Stock

 Additional

 Paid-In

 Accumulated

 Total

 Stockholders'

 Equity

 Shares

 Amount

 Capital

 Deficit

 (Deficit)

 Balance at December 31, 2022

 8,306,326

 $

 $
 508,584

 $
 ( 501,613
)

 $
 6,979

 -

 -

 -

)

)

 Common stock issued under 2023 At the Market Offering Agreement, net of issuance
 costs

 35,552

 -

 -

 Common stock issued under 2023 Equity Line of Credit Agreement, net of issuance costs

 360,943

 -

 1,177

 -

 1,177

 Common stock issued for entering into 2023 Equity Line of Credit Agreement

 47,733

 -

 -

 Common stock issued under 2023 Direct Offering, net of issuance costs

 1,694,820

 4,117

 -

 4,119

 Common stock issued for vested restricted stock awards

 199,699

 -

 Stock-based compensation expense

 -

 -

 -

 Fractional shares adjustment related to reverse stock split

 ( 24
)

 -

 -

 -

 -

 Balance at December 31, 2023

 10,645,049

 515,927

 ( 518,303
)

 ( 2,365
)

 Balance

 10,645,049

 $

 $
 515,927

 $
 ( 518,303
)

 $
 ( 2,365
)

 -

 -

 -

)

)

 Common stock issued under 2023 Equity Line of Credit Agreement

 949,574

 1,900

 -

 1,901

 Common stock issued under 2024 Direct Offering, net of issuance costs of $733

 1,371,000

 4,829

 -

 4,830

 Common stock issued under Warrant Inducement Agreement, net of issuance costs of $277

 1,711,111

 1,860

 -

 1,862

 Reclassification of Warrant Liability upon Exercise

 -

 -

 -

 Common stock issued under 2024 At the Market Agreement, net of issuance costs of $189

 1,073,050

 -

 Common stock issued under 2024 Private Placement Offering, net of issuance costs of $72

 1,248,527

 1,837

 -

 1,838

 Common stock issued under 2024 Securities Purchase Agreements

 9,733

 -

 -

 Warrant Exercise

 200,000

 -

 -

 -

 -

 Common stock issued for vested restricted stock awards

 199,076

 -

 -

 Stock-based compensation expense

 -

 -

 1,333

 -

 1,333

 Balance at December 31, 2024

 17,407,120

 $

 $
 528,817

 $
 ( 531,397
)

 $
 ( 2,563
)

 Balance

 17,407,120

 $

 $
 528,817

 $
 ( 531,397
)

 $
 ( 2,563
)

 See
accompanying notes to consolidated financial statements

 F- 4

 Consolidated
Statements of Cash Flows

 (Amounts
in Thousands)

 2024

 2023

 2024

 2023

 Cash flows from operating activities:

 $
)

 $
)

 Adjustments to reconcile net loss to net cash used in operating activities:

 -

 Non-cash lease expense

 ( 9
)

 Depreciation and amortization

 Stock-based compensation expense

 1,494

 1,724

 Change in fair value of warrant liabilities

 ( 1,346
)

 ( 629
)

 Loss on impairment and disposal of property and equipment

 Forgiveness of DECD loan

 -

 ( 1,000
)

 Financing expense for entering into equity line of credit with Lincoln Park

 -

 Changes in operating assets and liabilities:

 Accounts receivable

 ( 214
)

 Prepaid expenses and other assets

 ( 115
)

 Inventories

 ( 99
)

 Accounts payable

 Accrued liabilities

 ( 418
)

 ( 539
)

 Other liabilities

 ( 52
)

 ( 68
)

 Net cash used in operating activities

 ( 12,113
)

 ( 15,894
)

 Cash flows from investing activities:

 Purchase of property and equipment

 ( 37
)

 ( 24
)

 Net cash used in investing activities

 ( 37
)

 ( 24
)

 Cash flows from financing activities:

 Principal repayment of DECD loan

 ( 93
)

 ( 148
)

 Proceeds from 2023 At the Market Offering Agreement

 -

 Payment of issuance costs for 2023 At the Market Offering Agreement

 -

 ( 134
)

 Proceeds from 2023 Equity Line of Credit Agreement

 1,901

 1,177

 Proceeds from 2023 Direct Offering

 -

 4,716

 Payment of issuance costs for 2023 Direct Offering

 -

 ( 597
)

 Proceeds from 2024 Direct Offering

 5,563

 -

 Payment of issuance costs for 2024 Direct Offering

 ( 733
)

 -

 Proceeds from Warrant Inducement Agreement

 2,139

 -

 Payment of issuance costs for Warrant Inducement Agreement

 ( 277
)

 -

 Proceeds from 2024 Securities Purchase Agreements

 -

 Proceeds from 2024 At the Market Offering Agreement

 -

 Payment of issuance costs for 2024 At the Market Offering Agreement

 ( 189
)

 -

 Proceeds from 2024 Private Placement Offering

 1,910

 -

 Proceeds from issuance of common stock

 1,910

 -

 Payment of issuance costs for 2024 Private Placement Offering

 ( 72
)

 -

 Payment of stock issuance costs

 ( 72
)

 -

 Net cash provided by financing activities

 11,064

 5,216

 Net decrease in cash, cash equivalents and restricted cash

 ( 1,086
)

 ( 10,702
)

 Cash, cash equivalents and restricted cash, beginning of year

 2,855

 13,557

 Cash, cash equivalents and restricted cash, end of year

 $
 1,769

 $
 2,855

 Reconciliation to Consolidated Balance Sheet:

 Cash and cash equivalents

 $
 1,769

 $
 2,597

 Restricted cash

 -

 Unrestricted and restricted cash and cash equivalents

 $
 1,769

 $
 2,855

 Supplemental disclosure of cash flow information:

 Cash paid for interest

 $

 $

 Supplemental disclosure of noncash investing and financing activities:

 Forgiveness of DECD loan

 $
 -

 $
 ( 1,000
)

 Warrants modification - incremental value

 $
 1,323

 $
 -

 Commitment shares for equity line of credit

 $
 -

 $

 Increase in right-of-use assets

 $

 $

 See
accompanying notes to consolidated financial statements

 F- 5

 Notes
to Consolidated Financial Statements

 NOTE
1: BASIS OF PRESENTATION AND SUMMARY OF SIGNIFICANT ACCOUNTING AND REPORTING POLICIES

 Organization
and Basis of Presentation

Women's Health Inc. ("Aspira," and together with its wholly-owned subsidiaries,
the "Company") is incorporated in the state of Delaware, and is engaged in the business of developing and commercializing
diagnostic tests for gynecologic disease. The Company currently markets and sells the following products and related services: (1) the
Ova1Plus workflow, which uses Ova1, a qualitative serum test intended as an aid to further assess the likelihood of malignancy in women
with an ovarian adnexal mass for which surgery is planned when the physician's independent clinical and radiological evaluation
does not indicate malignancy, as the primary test and Overa, a second-generation biomarker test intended to maintain Ova1's high
sensitivity while improving specificity, as a reflex for Ova1 intermediate range results, leveraging the strengths of Ova1's MIA
sensitivity and Overa's (MIA2G) specificity to reduce incorrectly elevated results; and (2) OvaWatch, an LDT intended to assist
in the initial clinical assessment of malignancy risk in all women thought to have an indeterminate or benign adnexal mass. Overa is
currently not offered except as a reflex test performed as part of the Ova1Plus workflow. Collectively, these tests are referred to and
marketed as OvaSuite. The Company's products are distributed through its own national sales force, through its proprietary decentralized testing platform
and cloud service marketed as Aspira Synergy, and through marketing and distribution agreements with BioReference Health, LLC and ARUP
Laboratories.

 Operating segments are defined
as components of a business about which separate discrete information is available and used for evaluation by the chief operating decision
maker (the "CODM") in deciding how to allocate resources and assess performance. The company's chief executive officer
alone is the Company's CODM. Refer to Note 12 – Segment Reporting for more information.

 Going
Concern

 The
Company has incurred significant net losses and negative cash flows from operations since inception, and as a result has an accumulated
deficit of approximately $ 531,397,000 and working capital deficit of $ 1,285,000 as of December 31, 2024. For the year ended December
31, 2024, the Company incurred a net loss of $ 13,094,000 and used cash in operations of $ 12,113,000. The Company also expects to incur
a net loss and negative cash flows from operations for 2025. In order to continue its operations as currently planned through 2025 and
beyond, the Company will need to raise additional capital. The Company expects to take further action to protect its liquidity position.
Such actions may include, but are not limited to:

 Raising
 capital through an equity offering either in the public markets or via a private placement
 offering (to the extent that the Company raises additional funds by issuing equity securities,
 the Company's stockholders may experience significant dilution. However, no assurance
 can be given that capital will be available on acceptable terms, or at all);

 Securing
 debt, however, no assurance can be given that debt will be available on acceptable terms
 or at all;

 Reducing
 executive bonuses or replacing cash compensation with equity grants;

 Reducing
 professional services and consulting fees and eliminating non-critical projects;

 Reducing
 travel and entertainment expenses; and

 Reducing,
 eliminating or deferring discretionary marketing programs.

 The
Company also has outstanding warrants to purchase shares of its common stock that may be exercised; although there can be no assurance
that the warrants will be exercised.

 There
can be no assurance that the Company will achieve or sustain profitability or positive cash flow from operations. Management expects
cash from product sales and licensing to be the Company's only material, recurring source of cash in 2025. Given the above conditions,
there is substantial doubt about the Company's ability to continue as a going concern within one year after the date these financial
statements are filed. The consolidated financial statements have been prepared on a going concern basis and do not include any adjustments
that might result from these uncertainties.

 F- 6

 On
July 1, 2024, the Company received a deficiency letter from the Listing Qualifications Department of the Nasdaq Stock Market ("Nasdaq")
notifying the Company that, for the 30 consecutive business days prior to the date of the deficiency letter, the Company's Market
Value of Listed Securities was below the minimum of $ 35
 million requirement for continued inclusion on
The Nasdaq Capital Market pursuant to Nasdaq Listing Rule 5550(b)(2) (the "MVLS Requirement"). In accordance with Nasdaq
Listing Rule 5810(c)(3)(C), Nasdaq provided the Company with 180 calendar days, or until December 30, 2024, to regain compliance with
the MVLS Requirement. On December 31, 2024, the Company received written notice from the Staff of Nasdaq notifying it that the Company
failed to regain compliance with the MVLS Requirement by the Compliance Date. As such, the Company requested an appeal of Nasdaq's
determination to delist the Company's common stock from The Nasdaq Capital Market and paid Nasdaq a hearing fee of $ 20,000.
The hearing was held on February 18, 2025.

 The Company presented an appeal of Nasdaq's determination to delist its common stock. As a result of the hearing,
on March 6, 2025, the Company received written notice from Nasdaq that it would grant the Company's request for continued listing
on the Nasdaq Capital Market subject to certain conditions. Although the Company has been granted the conditional exception to remain
listed on the Nasdaq Capital Market, no assurance can be provided that it will successfully meet the conditions of the exception and that
its common stock will continue to be listed on The Nasdaq Capital Market.

 On October
17, 2024, the Company received a second deficiency letter from the Listing Qualifications Department of The Nasdaq Stock Market
stating that, for the preceding 30 consecutive business days, the closing bid price for Aspira common stock was below the minimum
$ 1.00 per share
requirement for continued inclusion on The Nasdaq Capital Market pursuant to Nasdaq Listing Rule 5550(a)(2). As provided in the
Nasdaq rules, the Company has 180 calendar days, or until April 15, 2025, to regain compliance with the Minimum Bid Price Rule. The
Company may achieve compliance during this period if the closing bid price of Aspira common stock is at least $ 1.00
per share for a minimum of 10 consecutive business days. If the Company fails to regain compliance on or prior to April 15, 2025,
the Company may be eligible for an additional 180-calendar day compliance period, which would extend the deadline until October 12,
2025. There is no assurance that the Company will be able to regain compliance by the April 15, 2025 deadline or the additional
180-calendar day extended deadline, and there is no assurance that the Company will otherwise maintain compliance with this or any
of the other Nasdaq continued listing requirements.

 On February 11, 2025, the Company
received written notice from the Nasdaq Stock Market, LLC ("Nasdaq") that based on the closing bid price per share immediately
preceding entering into a binding agreement to issue the securities for the Private Placement of $ 1.47 per share plus $ 0.125 attributable
to the value of the warrants, the market value of the transaction for purposes of Listing Rule 5625(c) was $ 1.595. Since the shares and
warrants sold in the private placement were issued below the market value, and the Company failed to obtain shareholder approval, the
Company violated Listing Rule 5635(c). Accordingly, this matter served as an additional basis for delisting the Company's securities
from The Nasdaq Stock Market.

 Subsequently, on February 11,
2025, the Company completed amendments to the warrants prohibiting exercise until shareholder approval has been obtained. As a result,
the Staff of Nasdaq has determined that the Company has regained compliance with Listing Rule 5635(c) and subject to the disclosure requirements
below, this matter is now closed.

 Basis
of Consolidation

 The
consolidated financial statements include the accounts of the Company and its wholly-owned subsidiaries. All intercompany transactions
have been eliminated in consolidation.

 Use
of Estimates

 The
preparation of consolidated financial statements in accordance with generally accepted accounting principles in the U.S. ("GAAP")
requires management to make estimates and assumptions that affect the amounts reported in the consolidated financial statements and accompanying
notes. The primary estimates underlying the Company's consolidated financial statements include assumptions regarding revenue recognition
as well as variables used in calculating the fair value of the Company's equity awards, warrants, income taxes and contingent liabilities.
Changes in estimates and assumptions are reflected in reported results in the period in which they become known. Actual results could
differ from those estimates.

 Cash
and Cash Equivalents

 Cash
and cash equivalents consist of cash and highly liquid investments with maturities of three months or less from the date of purchase,
which are readily convertible into known amounts of cash and are so near to their maturity that they present an insignificant risk of
changes in value because of interest rate changes. Highly liquid investments that are considered cash equivalents include money market
funds.

 Restricted
Cash

 Restricted
cash consists of a security deposit for a credit card financing arrangement. The restriction on the cash was removed when the Company
closed its credit card account.

 Fair
Value Measurements

 Accounting
Standards Codification ("ASC") Topic 820, Fair Value and Measurements ("ASC 820"), defines fair value
as the exchange price that would be received for an asset or paid to transfer a liability (an exit price) in the principal or most advantageous
market for the asset or liability in an orderly transaction between market participants on the measurement date. ASC 820 also establishes
a fair value hierarchy which requires an entity to maximize the use of observable inputs and minimize the use of unobservable inputs
when measuring fair value. The standard describes three levels of inputs that may be used to measure fair value:

 Level
1 – Quoted prices in active markets for identical assets or liabilities.

 F- 7

 Level
2 – Observable inputs other than Level 1 prices such as quoted prices for similar assets or liabilities, quoted prices in markets
that are not active, or other inputs that are observable or can be corroborated by observable market data for substantially the full
term of the assets or liabilities.

 Level
3 – Unobservable inputs that are supported by little or no market activity and that are significant to the fair value of the assets
or liabilities.

 If
a financial instrument uses inputs that fall within different levels of the hierarchy, the instrument will be categorized based upon the
lowest level of input that is significant to the fair value calculation.

 Financial
instruments of the Company consist primarily of cash and cash equivalents, restricted cash, accounts receivable, accounts payable, debt
and warrant liabilities.

 The estimated fair
value of financial instruments has been determined using available market information or other appropriate valuation methodologies. However,
considerable judgment is required in interpreting market data to develop estimates of fair value; therefore, the estimates are not necessarily
indicative of the amounts that could be realized or would be paid in a current market exchange. The effect of using different market
assumptions and/or estimation methodologies may be material to the estimated fair value amounts.

 Cash
and cash equivalents, restricted cash, accounts receivable, and accounts payable are considered Level 1 and are carried at cost due
to their short-term nature and their market interest rates. Warrant liabilities are considered Level 2 and are recorded at fair value at
the end of each reporting period. Debt is considered Level 3, which the Company does not record at fair value. The carrying value of
debt approximates fair value due to its interest rate approximating market rates of interest available to the Company for similar
instruments.

 Concentration
of Credit Risk

 Financial
instruments that potentially subject the Company to a concentration of credit risk consist of cash and cash equivalents and accounts
receivable. The Company maintains cash and cash equivalents in recognized financial institutions in the United States. The funds are
insured by the FDIC up to a maximum of $ 250,000 but are otherwise unprotected. The Company has not experienced any losses associated
with deposits of cash and cash equivalents. The Company does not invest in derivative instruments or engage in hedging activities.

 Accounts
Receivable

 Virtually
all accounts receivable are derived from sales made to customers located in North America. The Company grants credit to customers in
the normal course of business and the resulting trade receivables are stated at their net realizable value. The Company maintains an
allowance for credit losses based upon the expected collectability of accounts receivable, such as the historical collection cycle. Amounts
are written off against the allowances for credit losses when the Company determines that a customer account is not collectable. We believe
our exposure to concentrations of credit risk is limited due to the diversity of our payer base.

 Inventory

 The
Company has inventory consisting primarily of kit inventory for specimen delivery as well as reagents used for specimen testing and miscellaneous
inventory such as pipettes, gloves and other non-reagent items.

 At
each reporting period the Company reviews its inventories for obsolescence and writes down obsolete or otherwise unmarketable inventory
to its estimated net realized value, which is primarily related to kit inventory when kits expire. Inventory is valued at cost using
the first-in-first-out method.

 Property
and Equipment

 Property
and equipment are carried at cost less accumulated depreciation and amortization. Property and equipment are depreciated when placed
into service using the straight-line method over the estimated useful lives, generally three to five years. Leasehold improvements are
amortized using the straight-line method over the shorter of the estimated useful life of the asset or the remaining term of the lease.
Maintenance and repairs are charged to operations as incurred. Upon sale or retirement of assets, the cost and related accumulated depreciation
are removed from the balance sheet and the resulting gain or loss is reflected in operations.

 Property
and equipment are reviewed for impairment when events or changes in circumstances indicate the carrying amount of an asset may not be
recoverable. If property and equipment are considered to be impaired, an impairment loss is recognized.

 F- 8

 Revenue
Recognition

Revenue – OvaSuite: The Company recognizes product revenue in accordance with the provisions of ASC Topic 606, Revenue from
Contracts with Customers ("ASC 606"). Product revenue is recognized upon completion of the OvaSuite test and delivery
of results to the physician based on estimates of amounts that will ultimately be realized. In determining the amount of revenue to be
recognized for a delivered test result, the Company considers factors such as payment history and amount, payer coverage, whether there
is a reimbursement contract between the payer and the Company, and any developments or changes that could impact reimbursement. These
estimates require significant judgment by management as the collection cycle on some accounts can be as long as one year. The effect
of any change made to an estimated input component and, therefore revenue recognized, would be recorded as a change in estimate at the
time of the change.

 The
Company also reviews its patient account population and determines an appropriate distribution of patient accounts by payer (i.e., Medicare,
patient pay, other third-party payer, etc.) into portfolios with similar collection experience. The Company has elected this practical
expedient that, when evaluated for collectability, results in a materially consistent revenue amount for such portfolios as if each patient
account were evaluated on an individual contract basis. During the years ended December 31, 2024 and 2023, there were no adjustments
to estimates of variable consideration to derecognize revenue for services provided in a prior period; however, additional revenue of approximately $ 4,000 and $ 87,000 was recognized
during the years ended December 31, 2024 and 2023, respectively. There were no impairment losses
on accounts receivable recorded during the years ended December 31, 2024 and 2023.

 Research
and Development Costs

 Research
and development costs are expensed as incurred. Research and development costs consist primarily of payroll and related costs, materials
and supplies used in the development of new products, and fees paid to third parties that conduct certain research and development activities
on behalf of the Company. In addition, acquisitions of assets to be consumed in research and development, with no alternative future
use, are expensed as incurred as research and development costs. Software development costs incurred in the research and development
of new products are expensed as incurred until technological feasibility is established.

 Patent
Costs

 Costs
incurred in filing, prosecuting and maintaining patents (principally legal fees) are expensed as incurred and recorded within general
and administrative expenses on the Company's consolidated statements of operations. Such costs aggregated to approximately $ 274,000
and $ 341,000
for the years ended December 31, 2024 and 2023, respectively.

 Stock-Based
Compensation

 The
Company records the fair value of non-cash stock-based compensation costs for stock options related to the 2019 Stock Incentive Plan
("2019 Plan"). The Company estimates the fair value of stock options using a Black-Scholes option valuation model. This model
requires the input of subjective assumptions including expected stock price volatility, expected life and estimated forfeitures of each
award. These assumptions consist of estimates of future market conditions, which are inherently uncertain, and therefore are subject
to management's judgment. The Company uses the straight-line method to amortize the fair value over the requisite service period
of the award, which is generally equal to the vesting period.

 The
expected life of options is based on historical data of actual experience with the options granted and represents the period of time
that the options granted are expected to be outstanding. This data includes employees' expected exercise and post-vesting employment
termination behaviors. The expected stock price volatility is estimated using Company historical volatility in deriving the expected
volatility assumption. The Company made an assessment that Company historic volatility is most representative of future stock price trends.
The expected dividend yield is based on the estimated annual dividends that are expected to be paid over the expected life of the options
as a percentage of the market value of the Company's common stock as of the grant date. The risk-free interest rate for the expected
life of the options granted is based on the United States Treasury yield curve in effect as of the grant date. The Company records stock-based
compensation net of estimated forfeitures.

 F- 9

 2023
Reverse Stock Split

 At
the Company's annual meeting on May 9, 2023, the stockholders of the Company approved the proposal to authorize the board of directors
in its discretion, without further authorization of the Company's stockholders, to amend the Company's Certificate of Incorporation
to effect a reverse split of the Company's common stock by a ratio of between one-for-ten and one-for-twenty. On May 9, 2023, the
Company's board of directors approved a one-for-fifteen reverse stock split of the Company's common stock without any change
to its par value, which became effective on May 12, 2023. All references to share and per share amounts for all periods presented in
these consolidated financial statements have been retrospectively restated to reflect the Reverse Stock Split and proportional adjustment
of the preferred stock conversion ratio. Par values were not adjusted.

 Government
Assistance

 On
March 27, 2020, the U.S. government enacted the Coronavirus Aid, Relief, and Economic Security Act ("CARES Act"). One provision
within the CARES Act provided an Employee Retention Credit ("ERC"), which allows for employers to claim a refundable
tax credit against the employer share of Social Security tax equal to 50% of the qualified wages paid to employees from March 13, 2020
through December 31, 2020. The ERC was subsequently expanded in 2021 for employers to claim a refundable tax credit for 70% of the qualified
wages paid to employees from January 1, 2021 through September 30, 2021.

 The
Company qualified for federal government assistance through the ERC. During the years ended December 31, 2024 and 2023, the Company received
approximately $ 38,000 and $ 347,000, respectively, from the Internal Revenue Service for payroll tax refunds for 2020. The Company recorded
the receipt as other income in its consolidated statements of operations.

 In
October 2024, the Company was selected as an awardee of a federal health an initiative to address critical unmet challenges in women's
health, champion transformative innovations, and tackle health conditions that uniquely or disproportionately affect women. Under this
initiative, the Company expects to receive up to $ 10,000,000 in milestone-based funding over two years to develop its multi-marker blood test to
aid in the detection of endometriosis. The test will rely on an AI-powered algorithm that combines protein and microRNA biomarkers
and patient data, and leverage technology that the Company pioneered for its ovarian cancer risk assessment blood tests.

 The Company
met the first milestone for payment in the fourth quarter of 2024 and received a payment of $ 2,000,000. The award also provides for access
to a team of subject matter experts and advisors to support the successful completion and commercial launch of the test before
the end of the two-year contract term. The Company will work with a Program Manager in the design, development, and commercial launch of this
first-of-its kind endometriosis diagnostic test.

 Applying
guidance from IAS 20, the Company accounts for each milestone in the contract as an individual obligation. The Company recognizes income
when there is reasonable assurance that the entity will meet the conditions and that the grant will be received. Notwithstanding Aspira's
adoption of IAS 20 deferred income approach, due to the uncertainty posed by the current political climate, particularly with respect
to government awards, Aspira will recognize income for each milestone only upon receipt of payments. During the year ended December 31,
2024, the Company recorded other income of $ 2,000,000 in its consolidated statement of operations related to the award.

 Contingencies

 The
Company accounts for contingencies in accordance with ASC 450 Contingencies ("ASC 450") which requires that an estimated
loss from a loss contingency be accrued when (i) information available prior to issuance of the financial statements indicates that it
is probable that an asset has been impaired or a liability has been incurred at the date of the financial statements and (ii) when the
amount of the loss can be reasonably estimated. Accounting for contingencies such as legal and contract dispute matters requires the
use of management's judgment. Management believes that the Company's accruals for these matters are adequate. Nevertheless,
the actual loss from a loss contingency might differ from management's estimates.

 Income
Taxes

 The
Company accounts for income taxes using the asset and liability method. Under this method, deferred tax assets and liabilities are determined
based on the difference between the financial statement and the tax bases of assets and liabilities using the current tax laws and rates.
A valuation allowance is established when necessary to reduce deferred tax assets to the amounts more likely than not expected to be
realized.

 F- 10

 ASC
Topic 740, Accounting for Uncertainty in Income Taxes, clarifies the accounting for uncertainty in income taxes recognized in
the financial statements and provides that a tax benefit from an uncertain tax position may be recognized when it is more likely than
not that the position will be sustained upon examination, including resolutions of any related appeals or litigation processes, based
on the technical merits. This interpretation also provides guidance on measurement, derecognition, classification, interest and penalties,
accounting in interim periods, and disclosure.

 The
Company recognizes interest and penalties related to unrecognized tax benefits within the interest expense line and other expense line,
respectively, in its consolidated statements of operations. Accrued interest and penalties are included within the related liability
lines in the Company's consolidated balance sheet.

 Net
Loss Per Share

 Basic
net loss per share is computed by dividing the net loss by the weighted average number of shares of common stock outstanding during the
period. Diluted loss per share is computed by dividing the net loss by the weighted average number of shares of common stock adjusted
for the dilutive effect of common stock equivalent shares outstanding during the period. Common stock equivalents consist of stock options,
restricted stock units and stock warrants. Common equivalent shares are excluded from the computation in periods in which they have an
anti-dilutive effect on earnings per share.

 Leases

 The
Company determines if a contract, at its inception, is a lease or contains a lease based on whether the contract conveys the right to
control the use of identified property, plant, or equipment (an identified asset) for a period of time in exchange for consideration.
To determine whether a contract conveys the right to control the use of an identified asset for a period of time, the Company assesses
whether, throughout the period of use, it has both the right to obtain substantially all of the economic benefits from use of the identified
asset, and the right to direct the use of the identified asset.

 Lease
classification, recognition, and measurement are then determined at the lease commencement date. For arrangements that contain a lease
the Company (i) identifies lease and non-lease components, (ii) determines the consideration in the contract, (iii) determines whether
the lease is an operating or financing lease; and (iv) recognizes lease right-of-use assets and liabilities. Lease liabilities and their
corresponding right-of-use assets are recorded based on the present value of lease payments over the expected lease term. The interest
rate implicit in lease contracts is typically not readily determinable and as such, the Company uses an incremental borrowing rate based
on the information available at the lease commencement date, which represents a rate that would be incurred to borrow, on a collateralized
basis, over a similar term, an amount equal to the lease payments in a similar economic environment.

 The
Company enters into contracts that contain both lease and non-lease components. Non-lease components may include items such as maintenance,
utilities, or other operating costs. For leases of real estate, the Company combines the lease and associated non-lease components in
its lease arrangements as a single lease component. Variable costs, such as utilities or maintenance costs, are not included in the measurement
of right-of-use assets and lease liabilities, but rather are expensed when the event determining the amount of variable consideration
to be paid occurs.

 F- 11

 Additionally,
the Company has elected the short-term lease exemption and, therefore, does not recognize a right-of-use asset or corresponding liability
for lease arrangements with an original term of 12 months or less.

 Operating
leases are included in right-of-use operating assets, current lease liabilities, and noncurrent lease liabilities in the consolidated
balance sheets as of December 31, 2024 and 2023.

 Segment
Reporting

 The
Company's Chief Operating Decision Maker ("CODM") is its Chief Executive Officer. The Company views its operations
and manages its business in a single operating segment, which is the discovery, development, and commercialization of noninvasive diagnostic
tests. As a result, the CODM evaluates the business on a consolidated basis. The Company's CODM uses the net loss that is reported on the Company's
consolidated statement of operations as a consolidated net loss for the purpose of allocating resources. The Company also monitors its
cash and cash equivalents as reported on its consolidated balance sheets to determine its liquidity needs and to allocate resources. For
additional information, see Note 12, Segment Information.

 NOTE
2: RECENT ACCOUNTING PRONOUNCEMENTS

 In
August 2020, the FASB issued ASU No. 2020-06, Debt – Debt with Conversion and Other Options (Subtopic 470-20) and Derivatives
and Hedging – Contracts in Entity's Own Equity (Subtopic 815-40): Accounting for Convertible Instruments and Contracts in
an Entity's Own Equity ("ASU 2020-06"). This update was issued to assist in simplifying the accounting for convertible
instruments. The Company adopted ASU 2020-06 on January
1, 2024. The adoption
 of this standard did not
 have a material impact on the Company's
consolidated results of operations, financial position, or cash flows.

 In
June 2022, the FASB issued ASU No. 2022-03, Fair Value Measurement (Topic 820): Fair Value Measurement of Equity Securities Subject
to Contractual Sale Restrictions ("ASU 2022-03") to clarify guidance in Topic 820 on the fair value measurement of an
equity security that is subject to a contractual sale restriction and also requires specific disclosures related to an equity security.
ASU 2022-03 is scheduled to be effective for fiscal years beginning after December 15, 2024, including interim periods within those fiscal
years. Early adoption is permitted. The Company does not expect a material impact as a result of this standard on its results of operations,
financial position, or cash flows.

 F- 12

 In
March 2023, the FASB issued ASU No. 2023-01, Leases (Topic 842): Common Control Arrangements ("ASU 2023-01").
ASU 2023-01 clarified the accounting for leasehold improvements for leases under common control. The guidance is scheduled to be effective
for fiscal years beginning after December 15, 2024, including interim periods within those fiscal years. Early adoption is permitted.
The Company does not expect the adoption of this standard to have a material impact on its results of operations, financial position,
or cash flows.

 In
October 2023, the FASB issued ASU No. 2023-06, D isclosure Improvements: Codification Amendments in Response to the SEC's Disclosure
Update and Simplification Initiative ("ASU 2023-06"). The amendments in this ASU are expected to clarify or improve disclosure
and presentation requirements of a variety of ASC topics by aligning them with the SEC's regulations. ASU 2023-06 will become effective
for each amendment on the effective date of the SEC's corresponding disclosure rule changes. The Company does not expect ASU 2023-06
to have a material impact on its results of operations, financial position, or cash flows.

 In
November 2023, the FASB issued ASU No. 2023-07, Segment Reporting (Topic 280): Improvements to Reportable Segment Disclosures
("ASU 2023-07") to update reportable segment disclosure requirements, primarily through enhanced disclosures about significant
segment expenses and information used to assess segment performance. This ASU requires disclosure of significant segment expenses that
are regularly provided to the CODM and included within the reported measure of a segment's profit or loss, requires interim disclosures
about a reportable segment's profit or loss and assets that are currently required annually, requires disclosure of the position
and title of the CODM, clarifies circumstances in which an entity can disclose multiple segment measures of profit or loss, and contains
other disclosure requirements. ASU 2023-07 was adopted by the Company on January 1, 2024. The adoption of this standard did not have
a material impact on the Company's consolidated results of operations, financial position, or cash flows. For additional information, see Note 12, Segment Information.

 In
December 2023, the FASB issued ASU No. 2023-09, Income Taxes (Topic 740): Improvements to Income Tax Disclosures ("ASU 2023-09")
that addresses requests for improved income tax disclosures from investors that use the financial statements to make capital allocation
decisions. Public entities must adopt the new guidance for fiscal years beginning after December 15, 2024. The amendments in this ASU
must be applied on a retrospective basis to all prior periods presented in the financial statements and early adoption is permitted.
The Company does not expect ASU 2023-09 will have a material impact on its results of operations, financial position, or cash flows.

 In
November 2024, the FASB issued ASU No. 2024-03, Income Statement – Reporting Comprehensive Income-Expense Disaggregation Disclosures
(Subtopic 220-40): Disaggregation of Income Statement Expenses ("ASU 2024-03") that requires disaggregation of certain
expense captions into specified categories in disclosures within the footnotes to the financial statements. The standard will become
effective for annual periods beginning after December 15, 2026. The Company does not expect the adoption of ASU 2024-03 to have a material
impact on its results of operations, financial position or cash flows.

 NOTE
3: STRATEGIC ALLIANCE WITH QUEST DIAGNOSTICS INCORPORATED

 In
March 2015, the Company reached an agreement with Quest Diagnostics Incorporated ("Quest Diagnostics"). Pursuant to this
agreement, all Ova1 U.S. testing services for Quest Diagnostics customers were transferred to Aspira's wholly-owned subsidiary,
Aspira Labs, as of August 2015. Pursuant to this agreement, as amended as of December 8, 2022, Quest Diagnostics has continued to provide
blood draw and logistics support by transporting specimens to Aspira Labs for testing and is billed by Quest Diagnostics for services
performed. The purpose of the 2022 amendment was to add OvaWatch to the U.S testing services for Quest Diagnostics customers and to extend
the term of the agreement from March 11, 2023 to December 31, 2023. Under the terms of the agreement, as amended, the Company was required
to pay an annual fee of $ 75,000 for the services of a part-time Quest Diagnostics project manager. The parties are currently negotiating
a renewal agreement. As of December 31, 2024, the Company has $ 331,051 accrued and payable to Quest Diagnostics for phlebotomy services
rendered on behalf of the Company under the terms of the agreements.

 F- 13

 Note
4: PROPERTY AND EQUIPMENT

 The
components of property and equipment as of December 31, 2024 and 2023 were as follows:

 SCHEDULE OF PROPERTY AND EQUIPMENT

 Useful Life

 2024

 2023

 Estimated

 Useful Life

 2024

 2023

 Machinery and equipment

 3 - 5 years

 $

 $

 Computer equipment and software

 3 years

 1,377

 Furniture and fixtures

 5 years

 Leasehold improvements (1)

 (1)

 Gross property and equipment

 1,463

 1,981

 Accumulated depreciation and amortization

 ( 1,394
)

 ( 1,816
)

 Property and equipment, net

 $

 $

 (1)
 Lesser
 of remaining lease term or estimated useful life

 Depreciation
expense for property and equipment was $ 92,000 and $ 199,000 for the years ended December 31, 2024 and 2023, respectively.

 NOTE
5: ACCRUED LIABILITIES

 The
components of accrued liabilities as of December 31, 2024 and 2023 were as follows:

 SCHEDULE OF COMPONENTS OF ACCRUED LIABILITIES

 2024

 2023

 Payroll and benefits related expenses

 $
 1,448

 $
 1,189

 Collaboration and research agreements expenses

 Professional services

 Other accrued liabilities

 Total accrued liabilities

 $
 2,445

 $
 2,863

 NOTE
6: COMMITMENTS, CONTINGENCIES AND DEBT

 Loan
Agreement

 On
March 22, 2016, the Company entered into a loan agreement (as amended, the "DECD Loan Agreement") with the DECD, pursuant
to which the Company may borrow up to $ 4,000,000 from the DECD. The loan bears interest at a fixed rate of 2.0 % per annum and requires
equal monthly payments of principal and interest until maturity, which would have occurred on April 15, 2026. As security for the loan,
the Company has granted the DECD a blanket security interest in the Company's personal and intellectual property. The DECD's
security interest in the Company's intellectual property may be subordinated to a qualified institutional lender.

 The
loan may be prepaid at any time without premium or penalty. An initial disbursement of $ 2,000,000 ("Loan 1") was made to
the Company on April 15, 2016 under the DECD Loan Agreement. On December 3, 2020, the Company received a disbursement of the remaining
$ 2,000,000 ("Loan 2") under the DECD Loan Agreement, as the Company had achieved the target employment milestone necessary
to receive an additional $ 1,000,000 under the DECD Loan Agreement and the DECD determined to fund the remaining $ 1,000,000 under the
DECD Loan Agreement after concluding that the required revenue target would likely have been achieved in the first quarter of 2020 in
the absence of the impacts of COVID-19.

 F- 14

 Under
the terms of the DECD Loan Agreement, the Company was eligible for forgiveness of up to $ 1,500,000 of the principal amount of the loan
if it was able to achieve certain job creation and retention milestones by December 31, 2022. On June 26, 2023, the Company was notified
by the DECD that the Company satisfied all job creation and retention requirements under the loan agreement to receive forgiveness of
$ 1,000,000. During the year ended December 31, 2023, the Company recorded the $ 1,000,000 as other income in its consolidated statement of operations.
If the Company fails to maintain its Connecticut operations through March 22, 2026, the DECD may require early repayment of a portion
or all of the loan plus a penalty of 5 % of the total funded loan.

 On
June 6, 2023, the Company was granted a deferral of interest and principal payments on a portion of the remaining outstanding balances
through December 1, 2023. On January 30, 2024, the Company was granted an additional deferral of interest and principal payments on a
portion of the remaining outstanding balances through June 1, 2024. The Company determined the loan deferrals met the definition of a
troubled debt restructuring under ASC 470-60, Troubled Debt Restructurings by Debtors, as the Company was experiencing financial
difficulties, and the lenders granted a concession. The future undiscounted cash flows of the DECD loan after the loan deferrals exceeded
the carrying value of the DECD loan prior to the loan deferrals. As such no gain was recognized as a result of the deferrals.

 On
October 2, 2024, the Company executed an additional deferral agreement (the "October 2 Deferral"), which provided for both
the interest and principal payments on Loan 1 to be deferred for August and September 2024. Payments resumed in October 2024. The October
2 Deferral also provides for both the interest and principal payments on Loan 2 to be deferred from August 2024 to May 2027, with payments
resuming in June 2027. The Company determined these loan deferrals also met the definition of a troubled debt restructuring under ASC
470-60, Troubled Debt Restructurings by Debtors, as the Company was experiencing financial difficulties, and the lenders granted
a concession. The future undiscounted cash flows of the DECD loan after the loan deferrals exceeded the carrying value of the DECD loan
prior to the loan deferrals. As such, no gain was recognized as a result of the deferrals.

 Long-term
debt consisted of the following:

 SCHEDULE OF LONG-TERM DEBT

 2024

 2023

 DECD loan, net of issuance costs

 $
 1,507

 $
 1,596

 Less: Current portion, net of issuance costs

 ( 229
)

 ( 166
)

 Total long-term debt, net of issuance costs

 $
 1,278

 $
 1,430

 As
of December 31, 2024, the annual amounts of future minimum principal payments due under the Company's contractual obligation
are shown in the table below. Unamortized debt issuance costs for the DECD loan were $ 4,000 as
of December 31, 2024. Debt related to the insurance promissory note of $ 614,000,
as described below, is not included in the following table due to the insurance promissory note being cancelable.

OF ANNUAL AMOUNTS OF FUTURE MINIMUM PRINCIPAL PAYMENTS DUE UNDER CERTAIN CONTRACTUAL OBLIGATIONS

 Payments Due by Period

 Total

 2025

 2026

 2027

 2028

 2029

 Thereafter

 DECD Loan

 $
 1,511

 $

 $

 $

 $

 $

 $

 Total

 $
 1,511

 $

 $

 $

 $

 $

 $

 F- 15

 The
DECD loan is classified within Level 3 of the fair value hierarchy. The following table presents the carrying value and fair value of
the DECD loan. The fair value is estimated based on the discounted cash flows using the prevailing marketing interest rates.

OF CARRYING VALUE AND FAIR VALUE OF DECD LOAN

 2024

 2023

 Fair Value Hierarchy

 Carrying Value

 Fair Value

 Carrying Value

 Fair Value

 DECD loan

 Level 3

 $
 1,511

 $
 1,169

 $
 1,604

 $
 1,255

 Insurance
Notes

2024 and 2023, the Company entered into insurance promissory notes for the payment of insurance premiums at an interest rate of 7.52 %
and 7.79 % respectively, with an aggregate principal amount outstanding of approximately $ 614,000 and $ 670,000 as of December 31, 2024
and 2023, respectively. The amount outstanding in 2024 could be substantially offset by the cancellation of the related insurance coverage
which is classified in prepaid insurance. The 2024 notes are payable in nine monthly installments with a maturity date of August 1, 2025.
The 2024 notes are payable in nine monthly installments with a maturity date of August 1, 2025.

 The
carrying value of the Company's insurance promissory note approximates fair value at December 31, 2024 and 2023, due to the short-term
nature of the insurance note and are classified as Level 2 within the fair value hierarchy.

the year ended December 31, 2023, the Company received a $ 250,000 insurance reimbursement check related to research samples lost in a
power outage in the Trumbull, Connecticut office in January 2023. The Company recorded the reimbursement as other income in its consolidated
statement of operations.

 Operating
Leases

 The
Company leases facilities to support its business of discovering, developing and commercializing diagnostic tests in the fields of gynecologic
disease. The Company's principal facility, including the CLIA laboratory used by Aspira Labs, is located in Austin, Texas, and
an administrative office is also located in Shelton, Connecticut.

 In
December 2024, the Company extended the Austin, Texas lease for an additional 54 months. The lease renewal also expands the leased space. The Company's renewed lease expires on August 31, 2031, with the option to extend the lease for an additional
three years. The Company had previously extended the lease by 37 months in July 2023. Prior to the renewal in 2023, the Company's
Texas lease had a term of 12 months, and the Company has elected the policy of not recording leases on the balance sheet when the leases
have terms of 12 months or less. Through June 30, 2023, the Company recognized the lease payments in profit and loss on a straight-line
basis over the term of the lease, and variable lease payments in the period in which the obligation for the payments was incurred. Variable
lease costs represent our share of the landlord's operating expenses. Beginning in the third quarter of 2023, the Company added
the extended Austin, Texas lease to its balance sheet as a right-of-use asset. The Company is not reasonably certain that it will exercise
the three year renewal option beginning on September 1, 2031.

 In
October 2015, the Company entered into a lease agreement for a facility in Trumbull, Connecticut, which was renewed in September 2020.
On May 30, 2023, the Company entered into an agreement with the owner of its Trumbull, Connecticut offices to move to a more economical
location in Shelton, Connecticut. The new lease in Shelton, Connecticut cancelled and replaced the Trumbull, Connecticut office lease.
The new lease term is for five years, and its commencement date was October 1, 2023. Continuation of the lease after the lease term would
be on a month-to-month basis.

 In
January 2023, the Company entered into a new sublease agreement for an administrative facility in Palo Alto, California. The
Company's sublease term commenced in April 2023 and expired on May 31, 2024. The Company did not renew its lease with the
sublessor. The sublessor, Invitae, filed for bankruptcy on February 15, 2024. The Company has applied for a refund of its
approximately $ 10,000 security deposit with the bankruptcy court.

 F- 16

 The
expense associated with these operating leases for the years ended December 31, 2024 and 2023 is shown in the table below (in thousands).
Included in the amounts below are $ 58,000 of short term lease expenses related to rent and variable costs for one lease during 2023 prior
to its treatment as a right-of-use asset and $ 114,000 related to rent and variable costs during
2023.

OF EXPENSE ASSOCIATED WITH OPERATING LEASES

 Lease Cost

 Classification

 2024

 2023

 Operating rent expense

 Cost of revenue

 $

 $

 Variable rent expense

 Cost of revenue

 $

 $

 Cost of Revenue [Member]

 Based
on the Company's leases as of December 31, 2024, the table below sets forth the approximate future lease payments related to operating
leases with initial terms of one year or more (in thousands).

OF FUTURE LEASE PAYMENTS RELATED TO OPERATING LEASES

 Year

 Payments

 2025

 $

 2026

 2027

 2028

 2029

 Thereafter

 Total Operating Lease Payments

 1,773

 Less: Imputed Interest

 ( 358
)

 Present Value of Lease Liabilities

 1,415

 Less: Unused Tennant Improvement Allowance

 ( 160

)

 Less: Operating Lease Liability, current portion

 ( 7
)

 Operating Lease Liability, non-current portion

 $
 1,248

 Supplemental
disclosure of cash flow information related to leases for the years ended December 31, 2024 and 2023 is shown in the table below (in
thousands).

 SCHEDULE OF WEIGHTED-AVERAGE LEASE TERM AND DISCOUNT RATE

 2024

 2023

 Cash paid for amounts included in measurement of lease liabilities:

 Operating cash outflows relating to operating leases

 $

 $

 Weighted-average remaining lease term (in years)

 5.9

 3.6

 Weighted-average discount rate

 7.59
 %

 7.30
 %

 Non-cancelable
Royalty Obligations and Other Commitments

 The
Company is a party to an amended research collaboration agreement with The Johns Hopkins University School of Medicine under which
the Company licenses certain of its intellectual property directed at the discovery and validation of biomarkers in human subjects,
including but not limited to clinical application of biomarkers in the understanding, diagnosis and management of human disease.
Under the terms of the amended research collaboration agreement, Aspira is required to pay the greater of 4 %
royalties on net sales of diagnostic tests using the assigned patents or annual minimum royalties of $ 57,500.
Royalty expense for the years ended December 31, 2024 and 2023 totaled $ 293,000
and $ 324,000,
respectively, and is recorded in cost of revenue in the Company's consolidated statements of operations.

 F- 17

 On
December 16, 2024, the Company announced that its former Chief Executive Officer would be leaving the Company. The Company recorded $ 393,000
on its consolidated statement of operations as severance expense, including $ 375,000 representing cash severance payments for nine months,
which will be paid in equal biweekly installments for a period of nine months and $ 18,000 representing health and dental insurance payments
for nine months. As of December 31, 2024, the Company had approximately $ 356,000 accrued for severance and $ 18,000 accrued for health
and dental insurance.

 Business
Agreements

 In
August 2022, the Company entered into a sponsored research agreement with Harvard's Dana-Farber Cancer Institute, Brigham &
Women's Hospital, and Medical University of Lodz for the generation of a multi-omic, non-invasive diagnostic aid to identify endometriosis
based on circulating miRNAs and proteins. The results of this collaboration will be advanced, co-developed technology to guide medical
and clinical management of women presenting with symptoms of endometriosis. This collaboration is expected to accelerate the development
and commercialization of future endometriosis products, such as ENDOinform. The contract requires payments to be made upon the achievement
of certain milestones. Under the terms of and as further described in the agreement, payments of approximately $ 1,252,000 have or will
become due from the Company to the counterparties upon successful completion of certain deliverables in 2022 and 2023 as follows: 68 %
was paid in 2022, 15 % was paid in 2023, and the remaining 17 % will become payable upon completion of certain deliverables estimated to
occur in 2025. During the year ended December 31, 2024, no expense was recorded as research and development expense in the Company's
consolidated financial statement of operations for the project. During the year ended December 31, 2023, approximately $ 215,000, was
recorded as research and development expense in the Company's consolidated financial statement of operations for the project. From
the inception of the Dana-Faber, Brigham, Lodz Research Agreement through December 31, 2024, research and development expenses in the
cumulative amount of $ 1,083,000 have been recorded. From the inception of the Dana-Faber, Brigham, Lodz Research Agreement through December
31, 2024, the Company made payments totaling $ 1,040,000. Additional payments of $ 212,000 are due to the collaboration partners in 2025
under the terms of the agreement.

 On
March 20, 2023, the Company entered into a licensing agreement ("Dana-Faber, Brigham, Lodz License Agreement") with Harvard's
Dana-Farber Cancer Institute, Brigham & Women's Hospital, and Medical University of Lodz under which the Company will license
certain of its intellectual property to be used in the Company's OvaSuite product portfolio. Under the Dana-Faber, Brigham, Lodz
License Agreement, the Company paid an initial license fee of $ 75,000, which was recorded as research and development expense on the
Company's consolidated financial statement of operations, and then will pay a license maintenance fee of $ 50,000 on each anniversary
of the date of the Dana-Faber, Brigham, Lodz License Agreement. The Dana-Faber, Brigham, Lodz License Agreement also requires non-refundable
royalty payments of up to $ 1,350,000 based on certain regulatory approvals and commercialization milestones and further royalty payments
based on the net sales of the Company's products included under the Dana-Faber, Brigham, Lodz License Agreement. No milestones
have been reached as of December 31, 2024.

 Contingent
Liabilities

 From
time to time, the Company is involved in legal proceedings and regulatory proceedings arising from operations. The Company establishes
reserves for specific liabilities in connection with legal actions that management deems to be probable and estimable. The Company is
not currently a party to any proceeding, the adverse outcome of which would have a material adverse effect on the Company's financial
position or results of operations.

 NOTE
7: COMMON STOCK

 Additional
Shares Authorized

 On
February 6, 2023, the Company filed with the Secretary of State of the State of Delaware a Certificate of Amendment to the Company's
Fourth Amended and Restated Certificate of Incorporation, as amended, to increase the authorized number of shares of the Company's
common stock from 150,000,000 shares to 200,000,000 shares.

 F- 18

 2023
At the Market Offering

 On
February 10, 2023, the Company entered into a Controlled Equity Offering Sales Agreement (the "Cantor Sales Agreement"),
with Cantor Fitzgerald & Co. ("Cantor") as agent, pursuant to which it could offer and sell, from time to time, through
Cantor, shares of the Company's common stock, par value $ 0.001 per share, having an aggregate offering price of up to $ 12.5 million
(the "Placement Shares").

 Under
the Cantor Sales Agreement, Cantor could sell the Placement Shares by any method permitted by law and deemed to be an "at the market
offering" as defined in Rule 415 promulgated under the Securities Act of 1933, as amended, or the Securities Act, including sales
made directly on the Nasdaq Capital Market, on any other existing trading market for the Company's common stock or to or through
a market maker or in privately negotiated transactions. Cantor received a Placement Fee of 3 %
for each completed sale of Placement Shares under the Cantor Sales Agreement. The Company was not obligated to make any sales
of the Placement Shares under the Cantor Sales Agreement.

the year ended December 31, 2023, the Company sold 35,552 shares of the Placement Shares, for gross proceeds of approximately $ 211,000.
For the year ended December 31, 2023, the Company recorded $ 134,000 as an offset to additional paid-in capital representing transaction-related
offering costs of the Placement Shares.

 In
connection with a follow-on equity offering on July 24, 2023, the Company delivered written notice to Cantor on July 19, 2023 that it
was suspending the prospectus supplement, dated February 10, 2023, related to the Company's common stock issuable under the Cantor
Sales Agreement. The 2023 At the Market Agreement was terminated in August 2024.

 2023
Equity Line of Credit

 On
March 28, 2023, the Company entered into a purchase agreement (the "2023 Equity Line of Credit Agreement") with Lincoln Park
Capital Fund, LLC ("Lincoln Park") and a registration rights agreement (the "LPC Registration Rights Agreement"),
pursuant to which the Company has the right, in its sole discretion, to sell to Lincoln Park shares of the Company's common stock,
par value $ 0.001 per share (the "Common Stock"), having an aggregate value of up to $ 10,000,000 (the "Purchase Shares"),
subject to certain limitations and conditions set forth in the 2023 Equity Line of Credit Agreement. The Company will control the timing
and amount of any sales of Purchase Shares to Lincoln Park pursuant to the 2023 Equity Line of Credit Agreement.

 Under
the 2023 Equity Line of Credit Agreement, on any business day after March 28, 2023 selected by the Company over the 36 -month term of
the 2023 Equity Line of Credit Agreement (each, a "Purchase Date"), the Company may direct Lincoln Park to purchase up to
 6,667 shares of Common Stock on such Purchase Date (a "Regular Purchase"); provided, however, that (i) a Regular Purchase
may be increased to up to 13,333 shares, if the closing sale price per share of the Common Stock on The Nasdaq Capital Market is not
below $ 7.50 on the applicable Purchase Date; (ii) a Regular Purchase may be increased to up to 16,666 shares, if the closing sale price
per share of the Common Stock on The Nasdaq Capital Market is not below $ 11.25 on the applicable Purchase Date; and (iii) a Regular Purchase
may be increased to up to 20,000 shares, if the closing sale price per share of the Common Stock on The Nasdaq Capital Market is not
below $ 15.00 on the applicable Purchase Date. In any case, Lincoln Park's maximum obligation under any single Regular Purchase
will not exceed $ 1,000,000. The above-referenced share amount limitations and closing sale price thresholds are subject to adjustment
for any reorganization, recapitalization, non-cash dividend, stock split, reverse stock split or other similar transaction as provided
in the 2023 Equity Line of Credit Agreement. The purchase price per share for each such Regular Purchase will be equal to the lesser
of:

 1.

 the
 lowest sale price for the Common Stock on The Nasdaq Capital Market on the date of sale; and

 F- 19

 2.

 the
 average of the three lowest closing sale prices for the Common Stock on The Nasdaq Capital Market during the 10 consecutive business
 days ending on the business day immediately preceding the purchase date.

 The
Company also has the right to direct Lincoln Park, on any business day on which the Company has properly submitted a Regular Purchase
notice for the maximum amount the Company is then permitted to sell to Lincoln Park in such Regular Purchase, to purchase an additional
amount of the Common Stock (an "Accelerated Purchase") of additional shares based on criteria established in the 2023 Equity
Line of Credit Agreement. An Accelerated Purchase, which is at the Company's sole discretion, may be subject to additional requirements
and discounts if certain conditions are met as defined in the 2023 Equity Line of Credit Agreement.

 The
issuance of the Purchase Shares had been previously registered pursuant to the Company's effective shelf registration statement
on Form S-3 (File No. 333-252267) (the "Old Registration Statement"), and the related base prospectus included in the Registration
Statement, as supplemented by a prospectus supplement filed on March 28, 2023, that has expired. On April 22, 2024, the Company filed
a registration statement on Form S-3 (File No. 333-278867) (the "Registration Statement"), and the related base prospectus
included in the Registration Statement, that was declared effective by the SEC on April 25, 2024.

the year ended December 31, 2023, the Company sold 472,312
shares of Common Stock under the 2023 Equity Line of Credit Agreement for gross proceeds of approximately $ 1,578,000
under the Old Registration Statement. In addition, 47,733
shares of Common Stock were issued to Lincoln Park as consideration for entering into the 2023 Equity Line of Credit
Agreement.

the year ended December 31, 2024, the Company sold 949,574
shares under the 2023 Equity Line of Credit Agreement for gross proceeds of approximately $ 1,900,000.
Over the life of the 2023 Equity Line of Credit Agreement through December 31, 2024, the Company sold 1,310,517
shares for gross proceeds of approximately $ 3,078,000.
The Company incurred approximately $ 326,000
of costs related to the execution of the 2023 Equity Line of Credit Agreement, all of which are reflected in the unaudited condensed
consolidated financial statements. Of the total costs incurred, approximately $ 258,000
was paid in common stock to Lincoln Park for a commitment fee and $ 30,000
was paid for Lincoln Park expenses. These transaction costs were included in other expense in the Company's consolidated
statement of operations for the year ended December 31, 2023. The Company incurred approximately $ 249,000
and $ 41,000
for legal fees during the year ended December 31, 2024 and 2023, respectively, and included the costs in general and administrative
expenses on its consolidated statement of operations. Under the terms of a Warrant Inducement Agreement, the Company agreed not to sell
shares under the 2023 Equity Line of Credit Agreement for six months from the effective date of the Form S-3, which was September 3,
2024. As of March 25, 2025, the remaining availability under the 2023 Equity Line of Credit Agreement was $ 1,700,000
of shares of Common Stock that can be sold to Lincoln Park under the 2023 Equity Line of Credit Agreement, subject to the terms of
the 2023 Equity Line of Credit Agreement.

 2023
Registered Direct Offering

 On
July 20, 2023, the Company entered into a securities purchase agreement (the "2023 Direct Offering Agreement"), with several
investors relating to the issuance and sale of 1,694,820 shares of its common stock, par value $ 0.001 per share (the "2023 Direct
Offering").

to the 2023 Direct Offering Agreement, the Company issued 1,650,473 shares of common stock to certain investors at an offering price
of $ 2.75 per share, and 44,347 shares of common stock to its directors and executive officers at an offering price of $ 3.98 per share,
which was the consolidated closing bid price of the Company's common stock on The Nasdaq Capital Market on July 19, 2023. The aggregate
gross proceeds to the Company from the 2023 Direct Offering were approximately $ 4.7 million, before deducting placement agent fees and
other estimated expenses of $ 597,000 payable by the Company.

 The
Company engaged Alliance Global Partners ("AGP") to act as sole placement agent in the 2023 Direct Offering. The Company
paid the placement agent a cash fee equal to 7.0 % of the aggregate gross proceeds generated from the 2023 Direct Offering, except that,
with respect to proceeds from the sale of 182,447 shares of common stock to certain investors, including directors and executive officers
of the Company, the placement agent's cash fee was 3.5 %. The Company also reimbursed the placement agent for its accountable offering-related
legal expenses of $ 75,000 and a non-accountable expense allowance of $ 30,000.

 F- 20

 2024
Registered Direct Offering

 On
January 24, 2024, the Company entered into a securities purchase agreement (the "2024 Direct Offering Agreement"), with
several investors relating to the issuance and sale of 1,371,000
shares of its common stock, par value $ 0.001
per share, and pre-funded warrants to purchase 200,000
shares of Common Stock (the "Pre-Funded Warrants"), in a registered direct offering, together with accompanying warrants
to purchase 1,571,000
shares of Common Stock (the "January 2024 Purchase Warrants", and together with the Pre-Funded Warrants, the
"January 2024 Warrants") in a concurrent private placement (the "Concurrent Private Offering" and together
with the registered direct offering, the "2024 Direct Offering").

to the 2024 Direct Offering Agreement, the Company issued 1,368,600
shares of common stock to certain investors at an offering price of $ 3.50
per share, and 2,400
shares of common stock to an executive officer, at an offering price of $ 4.255
per share, which was the consolidated closing bid price of the Company's common stock on The Nasdaq Capital Market on January
24, 2024 of $ 4.13
per share plus $ 0.125
per January 2024 Purchase Warrant. The purchase price of each Pre-Funded Warrant is equal to the combined purchase price at which a
share of Common Stock and the accompanying January 2024 Purchase Warrant is sold in this 2024 Direct Offering, minus $ 0.0001.
The gross proceeds to the Company from the 2024 Direct Offering were approximately $ 5,563,000,
before deducting placement agent fees and other expenses of approximately $ 733,000
payable by the Company. The 2024 Direct Offering closed on January 26, 2024.

 The
Pre-Funded Warrants were exercisable at any time after the date of issuance and had an exercise price of $ 0.0001 per share. A holder
of Pre-Funded Warrants could not exercise the warrant if the holder, together with its affiliates, would beneficially own more than 9.99 %
of the number of shares of Common Stock outstanding immediately after giving effect to such exercise. A holder of Pre-Funded Warrants
may increase or decrease this percentage to a percentage not in excess of 9.99 % by providing at least 61 days' prior notice to
the Company. All of the Pre-Funded Warrants were exercised on February 6, 2024 for gross proceeds of $ 20.

 The January 2024 Purchase Warrants
have an exercise price of $4.13 per share and were exercisable beginning six months after issuance. 1,400,000 of the January 2024 Purchase
Warrants were exercised on August 1, 2024 under a Warrant Inducement Agreement at a reduced price of $1.25 per share.

 The
Company engaged AGP to act as sole placement agent in the 2024 Direct Offering. The Company paid the placement agent a cash fee equal
to 7.0 % of the aggregate gross proceeds generated from the 2024 Direct Offering, except that, with respect to proceeds raised in this
2024 Direct Offering from certain designated persons, AGP's cash fee is reduced to 3.5 % of such proceeds, and to reimburse certain
fees and expenses of the placement agent in connection with the 2024 Direct Offering. The Company also reimbursed the placement agent
for its accountable offering-related legal expenses of $ 75,000 and a non-accountable expense allowance of $ 30,000. Costs related to the
2024 Direct Offering were recorded as an offset to additional paid-in capital on the Company's balance sheet as of December 31,
2024.

 The
Company evaluated the Pre-Funded Warrants and the January 2024 Purchase Warrants and concluded that they met the criteria to be
classified as equity within additional paid-in-capital.

 The
Pre-Funded Warrants are equity classified because they (1) are freestanding financial instruments that are legally detachable and separately
exercisable from the common stock, (2) are immediately exercisable, (3) do not embody an obligation for the Company to repurchase its
shares, (4) permit the holder to receive a fixed number of shares of common stock upon exercise, (5) are indexed to the Company's
common stock and (6) meet the equity classification criteria.

 The
January 2024 Purchase Warrants are equity classified because they (1) are freestanding financial instruments that are legally
detachable and separately exercisable from the common stock, (2) do not embody an obligation for the Company to repurchase its
shares, (3) permit the holder to receive a fixed number of shares of common stock upon exercise, (4) are indexed to the
Company's common stock and (5) meet the equity classification criteria.

 Effective
upon the closing of the 2024 Direct Offering, the Company also amended certain existing warrants (the "August 2022
Warrants") to purchase up to an aggregate of 366,664
shares at an exercise price of $ 13.20
per share and a termination date of August 25, 2027, so that the amended August 2022 Warrants have a reduced exercise price of
$ 4.13
per share and a new termination date of January 26, 2029. The other terms of the amended August 2022 Warrants remain unchanged. The
Company performed an analysis of the fair value of the August 2022 Warrants immediately before and after the modification and the
increase in fair value of the August 2022 Warrants of $ 490,000
was recorded as a change in fair value of warrant liabilities in the Company's consolidated statement of
operations.

 F- 21

 Approximately
$ 106,000
of the costs related to the 2024 Direct Offering were allocated to the August 2022 Warrants and were recorded as other expense in
the Company's consolidated statement of operations.

 2024
Private Placement Offering

 On
July 1, 2024, the Company entered into a securities purchase agreement with certain investors in a private placement (the
"2024 Private Placement Offering"). Pursuant to the 2024 Private Placement Offering, the Company issued an aggregate of 1,248,529
shares of its common stock and accompanying warrants (the "July 2024 Purchase Warrants") to purchase an equal number of
shares of common stock at a price of $ 1.53
per share and accompanying warrant. The July 2024 Purchase Warrants have an exercise price of $ 2.25
per share and are exercisable until their expiration on the third anniversary of the issuance date. The gross proceeds to the
Company from the 2024 Private Placement Offering were approximately $ 1,909,000,
before deducting expenses of approximately $ 72,000
payable by the Company.

 The
Company evaluated the July 2024 Purchase Warrants and concluded that they met the criteria to be classified as equity within
additional paid-in-capital.

 The
July 2024 Purchase Warrants are equity classified because they (1) are freestanding financial instruments that are legally
detachable and separately exercisable from the common stock, (2) do not embody an obligation for the Company to repurchase its
shares, (3) permit the holder to receive a fixed number of shares of common stock upon exercise, (4) are indexed to the
Company's common stock and (5) meet the equity classification criteria.

 In February 2025, certain July 2024 Purchase Warrants were modified to require shareholder approval of the July 2024
Purchase Warrants prior to their becoming exercisable. Refer to Note 13 – Subsequent Events for more information.

 2024
At the Market Offering

 On
August 2, 2024, the Company entered into an agreement with H.C. Wainwright in connection with an At the Market offering agreement
(the "2024 At the Market Offering") to sell shares of its common stock ("Common Stock"), having an aggregate
sales price of up to $ 4,450,000,
from time to time, through an "at the market offering" program under which H.C. Wainwright acts as sales agent. The
Company pays Wainwright a commission rate equal to 3.0 %
of the aggregate gross proceeds from each sale of shares under the 2024 At the Market Offering. The Company has also reimbursed
H.C. Wainwright for certain specified expenses in connection with entering into the 2024 At the Market Offering.

the year ended December 31, 2024, the Company sold 1,073,050
shares under the 2024 At the Market Offering for gross proceeds of approximately $ 903,000.
The Company incurred approximately $ 240,000
of costs related to the execution of the 2024 At the Market Offering, all of which were recorded as an offset to additional paid-in
capital on the Company's balance sheet as of December 31, 2024.

 2024
Securities Purchase Agreements

 In
August 2024, the Company entered into securities purchase agreements with two shareholders under which it sold a total of 9,733 shares
of common stock and received proceeds of approximately $ 11,000.

 2024
Warrant Inducement Agreement

 On
July 31, 2024, the Company entered into a warrant inducement agreement (the "Warrant Inducement Agreement") with a certain
holder (the "Holder") of (i) warrants to purchase 311,111 shares of Common Stock dated August 22, 2022 (the "August
2022 Warrants") and (ii) warrants to purchase 1,400,000 shares of Common Stock dated January 26, 2024 (the "January 2024
Warrants"), pursuant to which the Holder agreed to exercise in cash the warrants held at a reduced exercise price of $ 1.25 per
share (reduced from $ 4.13 per share for the August 2022 Warrants and $ 4.13 for the January 2024 Warrants).

 As
an inducement to such exercise, the Company agreed to issue to the Holder new Common Stock warrants (collectively, the "August
2024 Purchase Warrants"), to purchase up to 2,566,667
shares of Common Stock. The August 2024 Purchase Warrants were exercisable immediately after issuance and will expire 5
years from the initial exercise date.

 F- 22

 The
transaction, which closed on August 1, 2024, resulted in net proceeds of approximately $ 1,862,000.
The Warrant Inducement Agreement was entered into to encourage the exercise of the August 2022 Warrants and January 2024 Purchase
Warrants in order to obtain capital for operations. The $ 1,323,000
incremental value transferred for the modification to both the August 2022 Warrants and January 2024 Purchase Warrants as a result
of the Warrant Inducement Amendment was accounted for as an equity issuance cost and recognized within additional paid in capital in
the audited consolidated balance sheets.

 The
Company evaluated the August 2024 Purchase Warrants and concluded that they met the criteria to be classified as equity within
additional paid-in-capital.

 The
August 2024 Purchase Warrants are equity classified because they (1) are freestanding financial instruments that are legally
detachable and separately exercisable from the common stock, (2) do not embody an obligation for the Company to repurchase its
shares, (3) permit the holder to receive a fixed number of shares of common stock upon exercise, (4) are indexed to the
Company's common stock and (5) meet the equity classification criteria.

 Under
the terms of the Warrant Inducement Agreement, the Company was prohibited from selling shares under the 2024 At the Market Offering
until September 30, 2024. It was also prohibited from selling shares under our 2023 equity line of credit for a period of six months
from the effective date of the Form S-3, which was September 3, 2024.

 Warrants

of the Company's Warrants are classified as a long-term Warrant liability on the Company's balance sheet. The fair values
of the Warrants as of December 31, 2024 and December 31, 2023 were $ 61,000 and $ 1,651,000, respectively. The fair value of the Warrants
was estimated using Black-Scholes pricing model based on the following assumptions:

OF FAIR VALUE OF WARRANTS USING BLACK-SCHOLES PRICING MODEL

 2024

 2023

 Unmodified Warrants

 Modified Warrants

 Dividend yield

 -
 %

 -
 %

 -
 %

 Volatility

 111.3
 %

 103.6
 %

 105.1
 %

 Risk-free interest rate

 4.22
 %

 4.28
 %

 3.93
 %

 Expected lives (years)

 2.64

 4.07

 3.64

 Weighted average fair value

 $
 0.101

 $
 0.162

 $
 2.064

 The
fair value of the Warrants, which were deemed to be derivative instruments due to the certain contingent put feature, was determined
using the Black-Scholes option pricing model. The Black-Scholes option pricing model was deemed to be an appropriate model due to the terms of the Warrants issued, including a
fixed term and exercise price.

 The
fair value of Warrants was affected by changes in inputs to the Black-Scholes option pricing model including the Company's stock
price, expected stock price volatility, the contractual term, and the risk-free interest rate. This model uses Level 2 inputs, including
stock price volatility, in the fair value hierarchy established by ASC 820 Fair Value Measurement.

 F- 23

 Warrants
outstanding as of December 31, 2024 and 2023 were as follows:

OF WARRANTS OUTSTANDING

 Number of Warrants Outstanding and Common Stock Underlying Warrants

 Exercise Price

 Issuance Date

 Expiration Date

 per Share

 2024

 2023

 Unmodified August 2022 Warrants (1)

 August 25, 2022

 August 25, 2027

 $
 13.20

 433,321

 799,985

 Modified August 2022 Warrants (1)

 August 25, 2022

 August 25, 2027

 $
 4.13

 55,553

 -

 January 2024 Purchase Warrants (2)

 January 26, 2024

 July 26, 2029

 $
 4.13

 171,000

 -

 July 2024 Purchase Warrants (2)

 July 9, 2024

 July 9, 2027

 $
 2.25

 1,248,527

 -

 August 2024 Purchase Warrants (2)

 August 1, 2024

 August 1, 2029

 $
 1.36

 2,566,667

 -

 4,475,068

 799,985

 (1)
 Liability
 classified

 (2)
 Equity
 classified

 NOTE
8: LOSS PER SHARE

 The
Company calculates basic loss per share using the weighted average number of shares of Aspira common stock outstanding during the
period. The Company considers the August 2022 Warrants, the January 2024 Purchase Warrants, the July 2024 Purchase Warrants and the
August 2024 Purchase Warrants to be participating securities, because holders of such instruments participate in the event a
dividend is paid on common stock. The holders of the August 2022 Warrants, the January 2024 Purchase Warrants, the July 2024 Purchase Warrants and the August 2024 Purchase Warrants do not have a contractual obligation to share in the
Company's losses. As such, losses are attributed entirely to common stockholders and for periods in which the Company has
reported a net loss, diluted loss per common share is the same as basic loss per common share.

OF RECONCILIATION OF NUMERATORS AND DENOMINATORS OF BASIC AND DILUTED LOSS PER SHARE

 2024

 2023

 2024

 2023

 Numerator:

 Net Loss

 $
)

 $
)

 Denominator:

 Shares used in computing net loss per share, basic and diluted

 14,134,626

 9,233,306

 Net loss per share, basic and diluted

 $
 ( 0.93
)

 $
 ( 1.81
)

 Due
to net losses for the years ended December 31, 2024 and 2023, diluted loss per share is calculated using the weighted average number
of common shares outstanding and excludes the effects of potential shares of common stock that are antidilutive.

 The
potential shares of common stock that have been excluded from the diluted loss per share calculation above for the years ended December
31, 2024 and 2023 were as follows:

OF POTENTIAL SHARES OF COMMON STOCK EXCLUDED FROM DILUTED LOSS PER SHARE CALCULATION

 2024

 2023

 Stock options

 876,249

 759,922

 Restricted stock units

 149,061

 59,463

 Warrants

 4,475,068

 799,985

 Potential common shares

 5,500,378

 1,619,370

 F- 24

 NOTE
9: EMPLOYEE SHARE BASED COMPENSATION AND BENEFIT PLANS

 2010
Stock Incentive Plan

 The
Company's employees, directors, and consultants were eligible to receive awards under the Vermillion, Inc. Second Amended and Restated
2010 Stock Incentive Plan, which was replaced by the 2019 Plan (as defined below) with respect to future equity grants. As of December
31, 2024, there were no shares of Aspira common stock available for future grants under the 2010 Plan.

 As
of December 31, 2024, a total of 14,907 shares were reserved for issuance with respect to outstanding stock options.

 2019
Stock Incentive Plan

 At
the Company's 2019 annual meeting of stockholders, the Company's stockholders approved the Vermillion, Inc. 2019 Stock Incentive
Plan, which was later amended to the Aspira Women's Health Inc. (the "2019 Plan"). The purposes of the 2019 Plan are
(i) to align the interests of the Company's stockholders and recipients of awards under the 2019 Plan by increasing the proprietary
interest of such recipients in the Company's growth and success; (ii) to advance the interests of the Company by attracting and
retaining non-employee directors, officers, other employees, consultants, independent contractors and agents; and (iii) to motivate such
persons to act in the long-term best interests of the Company and its stockholders. The 2019 Plan allows the Company to grant stock options,
stock appreciation rights, restricted stock, restricted stock units and performance awards to participants.

 Subject
to the terms and conditions of the 2019 Plan, the initial number of shares authorized for grants under the 2019 Plan is 699,485. In May
2023, the Company's stockholders approved an increase of 333,333 to the number of shares available for issuance under the 2019
Plan for a total of 1,032,818. To the extent an equity award granted under the 2019 Plan expires or otherwise terminates without having
been exercised or paid in full, or is settled in cash, the shares of common stock subject to such award will become available for future
grant under the 2019 Plan. As of December 31, 2024, there were 530,613 shares of Aspira common stock available for future grants under
the 2019 Plan.

 The
stock option activity under the 2010 and 2019 Plan for the years ended December 31, 2024 and 2023 was as follows:

OF STOCK OPTION ACTIVITY UNDER THE 2010 AND 2019 PLAN

 Number of Shares

 Weighted Average Exercise Price

 Aggregate Intrinsic Value

 Weighted Average Remaining Contractual Term

 Options outstanding at December 31, 2023

 759,922

 17.48

 $
 113,929

 6.06

 Options granted

 677,424

 2.24

 Options forfeited or expired

 ( 561,097
)

 16.84

 Options outstanding (1) at December 31, 2024

 876,249

 $
 6.11

 $
 -

 7.71

 Exercisable options at December 31, 2024

 542,685

 $
 7.24

 $
 -

 6.89

 (1)
 Options outstanding include options vested and expected to vest

 There
no options exercised during the years ended December 31, 2024 and 2023.

the year ended December 31, 2024, the Company granted option awards under the 2019 Plan with a weighted average grant date fair value
of $ 1.36.

 Assumptions
included in the fair value per share calculations during the year ended December 31, 2024, were (i) expected terms of one to three years,
(ii) one to three year treasury interest rates of 3.58 % to 4.96 % and (iii) market close prices ranging from $ 0.75 to $ 4.87. The Company
recorded $ 13,000 in forfeitures for the year ended December 31, 2024.

the year ended December 31, 2023, the Company granted option awards under the 2019 Plan with a weighted average grant date fair value
of $ 4.16.

 F- 25

 Assumptions
included in the fair value per share calculations during the year ended December 31, 2023, were (i) expected terms of one to five years,
(ii) one to five year treasury interest rates of 3.89 % to 5.49 % and (iii) market close prices ranging from $ 2.40 to $ 8.70. The Company
recorded $ 60,000 in forfeitures for the year ended December 31, 2023.

 The following table summarizes RSU activity for the 2019 Plan during the years ended December 31, 2024 and 2023.

 SUMMARY
OF RSU ACTIVITY

 Number of Shares

 Weighted Average Grant Date Fair Value per Share

 RSUs outstanding at December 31, 2023

 59,463

 $
 3.19

 RSUs granted

 318,951

 1.13

 RSUs vested and issued

 ( 199,076
)

 1.49

 RSUs forfeited or expired

 ( 30,277
)

 3.16

 RSUs outstanding at December 31, 2024

 149,061

 $
 1.02

 During the year ended December 31, 2023, the Company granted RSUs with a weighted average grant date fair value of
$ 947,950. The total fair value of RSUs vested was $ 326,000 and $ 811,000 for the year ended December 31, 2024 and 2023, respectively.

 Stock-based Compensation

 The
allocation of non-cash stock-based compensation expense by functional area for the year ended December 31, 2024 and 2023 was as follows.

OF ALLOCATION OF EMPLOYEE STOCK-BASED COMPENSATION EXPENSE BY FUNCTIONAL AREA

 2024

 2023

 Cost of revenue

 $

 $

 1,110

 1,319

 Total

 $
 1,494

 $
 1,724

 As
of December 31, 2024, total unrecognized compensation cost related to unvested stock option awards was approximately $ 294,000, and the
related weighted average period over which it is expected to be recognized was 1.85 years. As of December 31, 2024, there was $ 73,000
in unrecognized compensation costs related to restricted stock units, and the related weighted average period over which it is expected
to be recognized is 0.49 years.

 401(k)
Plan

 The
Company's 401(k) Plan allows eligible employees to defer up to an annual limit of the lesser of 90.0 % of eligible compensation
or a maximum contribution amount subject to the Internal Revenue Service annual contribution limit. The Company is not required to make
Company contributions under the 401(k) Plan. During the years ended December 31, 2024 and 2023, the Company did no t make Company contributions
to the 401(k) Plan.

 NOTE
10: INCOME TAXES

 There
was no current
income tax expense or benefit for the years ended December 31, 2024 or 2023 because of net losses during those years. These net losses
were generated from domestic operations. Loss from continuing operations before income taxes for the years ended December 31, 2024 and
2023 were $ 13,094,000
and $ 16,690,000,
respectively.

 Based
on the available objective evidence, management believes it is more likely than not that the net deferred tax assets will not be fully
realizable. Accordingly, the Company has provided a full valuation allowance against its net deferred tax assets at December 31, 2024
and 2023. Therefore, there was no deferred income tax expense or benefit for the years ended
December 31, 2024 or 2023.

 F- 26

 The
components of net deferred tax assets at December 31, 2024 and 2023 were as follows:

OF COMPONENTS OF NET DEFERRED TAX ASSETS (LIABILITIES)

 2024

 2023

 Deferred tax assets:

 Net operating losses

 $
 55,899

 $
 52,540

 Capitalized research expenses

 3,120

 3,432

 Fixed asset depreciation

 Other

 ASC 842 Right of Use Liability

 Total deferred tax assets

 60,340

 57,293

 Valuation allowance

 ( 60,055
)

 ( 57,170
)

 Deferred tax assets

 $

 $

 Deferred tax liabilities:

 ASC 842 Right of Use Asset

 $
 ( 285
)

 $
 ( 123
)

 Deferred tax liabilities

 $
 ( 285
)

 $
 ( 123
)

 Net deferred tax assets

 $
 -

 $
 -

 The Company's gross
deferred tax asset for the state net operating losses and the valuation allowance for the year ended December 31, 2024 have each been
reduced by $ 2,350,000 to apply Section 382 of the Internal Revenue Code of 1986, as amended ("Section 382") net operating
loss utilization limitation to the state of California net operating leases.

 The
reconciliation of the statutory federal income tax rate to the Company's effective tax rate for the years ended December 31, 2024
and 2023 was as follows:

 SCHEDULE OF RECONCILIATION OF
STATUTORY FEDERAL INCOME TAX RATE

 2024

 2023

 Tax at federal statutory rate

 %

 %

 State tax, net of federal benefit

 -

 Change in valuation allowance

 ( 22
)

 ( 17
)

 Change in warrant valuation

 Net operating loss reduction due to Section 382 limitation

 ( 1
)

 ( 1
)

 Permanent items

 ( 1
)

 ( 1
)

 Deferred adjustments, return to provision

 ( 2
)

 ( 3
)

 Effective income tax rate

 -
 %

 -
 %

 The
Company's ability to use its net operating loss and credit carryforwards to offset future taxable income is restricted due to ownership
change limitations that have occurred in the past, as required by Section 382, as well as similar state provisions. Net operating losses which are limited from offsetting any future taxable income under
Section 382 are not included in the gross deferred tax assets presented above.

 Legislation commonly referred
to as the Tax Cut and Jobs Act (H.R. 1) was enacted on December 22, 2017. As a result of the Tax Cuts and Jobs Act of 2017, federal net
operating losses ("NOLs") arising before January 1, 2018, and federal NOLs arising after January 1, 2018 are subject to different
rules.

 The
Company's pre-2018 federal NOLs of $ 66,980,000, which are not limited from offsetting future taxable income under Section 382,
will expire in varying amounts from 2025 through 2037, if not utilized, and can offset 100% of future taxable income for regular tax
purposes. The Company also has pre-2018 federal NOLs
of approximately $ 30,512,000 that will expire if not utilized within 20 years of being generated that are limited in offsetting future
taxable income under Section 382. A portion may still potentially be utilized before they expire, but the portion which will not be able
to be utilized prior to expiration has been removed from gross deferred tax assets. The Company's
federal NOLs of $ 129,547,000 arising on or after January 1, 2018, can generally be carried forward indefinitely but such federal NOL
carryforwards are permitted to be used in any table year to offset up to 80% of future taxable income annually. State NOLs will expire
in varying amounts from 2025 through 2044 if not utilized. The Company's ability to use its NOLs during this period will be dependent
on the Company's ability to generate taxable income, and portions of the Company's NOLs could expire before the Company generates
sufficient taxable income.

 The
valuation allowance was $ 60,096,000
and $ 57,170,000
at December 31, 2024 and 2023, respectively. The increase of approximately $ 2,926,000
between 2024 and 2023 is primarily due to adjustments to the domestic deferred tax assets related to net operating losses.

 F- 27

 The
Company files income tax returns in the U.S. and in various state jurisdictions with varying statutes of limitations. The Company has
not been audited by the Internal Revenue Service or any state income or franchise tax agency. As of December 31, 2024, the Company's
federal returns for the years ended 2022 through the current period and most state returns for the years ended 2021 through the current
period are still open to examination. In addition, all of the net operating losses and research and development credits generated in
years earlier than 2022 and 2021, respectively, are still subject to Internal Revenue Service audit. The federal and California tax returns
for the year ended December 31, 2023 reflect research and development carryforwards of $ 4,657,000
and $ 6,067,000,
respectively. The Company does not anticipate claiming any additional research and development credits for the year ended December 31,
2024.

 As
of December 31, 2024, the Company's gross unrecognized tax benefits are approximately $ 9,257,000
which are entirely attributable to research and development credits. A reconciliation of the change in the Company's
unrecognized tax benefits is as follows:

 SCHEDULE OF
RECONCILIATION OF CHANGES IN THE COMPANY'S UNRECOGNIZED TAX BENEFITS

 Federal Tax

 State Tax

 Total

 Balance at December 31, 2022

 $
 4,375

 $
 5,856

 $
 10,231

 Increase in tax position during 2023

 Balance at December 31, 2023

 4,657

 6,067

 10,724

 Balance

 $
 4,657

 $
 6,067

 $
 10,724

 Return to provision true up

 ( 282
)

 ( 211
)

 ( 493
)

 Increase in tax position

 ( 282)

 ( 211)

 ( 493)

 Decrease due to expirations during 2024

 ( 974
)

 -

 ( 974
)

 Decrease due to expirations

 ( 974)

 -

 ( 974)

 Balance at December 31, 2024

 $
 3,401

 $
 5,856

 $
 9,257

 Balance

 $
 3,401

 $
 5,856

 $
 9,257

 The
increase for the year ended December 31, 2024 is related to positions taken in that year. If the $ 9,257,000
of unrecognized income tax benefit is recognized, approximately $ 9,257,000
would impact the effective tax rate in the period in which each of the benefits is recognized.

 The
Company does not expect its unrecognized tax benefits to change significantly over the next 12 months. The Company recognizes interest
and penalties related to unrecognized tax benefits within the interest expense line and other expense line, respectively, in its consolidated
statement of operations and comprehensive loss. The Company has no t recorded any interest or penalties as a result of uncertain tax positions
as of December 31, 2024 and 2023. Accrued interest and penalties would be included within the related liability in the consolidated balance
sheet.

 NOTE
11: RELATED PARTY TRANSACTIONS

 On
December 1, 2023, the Company entered into a consulting agreement with Biodesix, Inc. (the "Biodesix Agreement") to
assist with our miRNA product pipeline. Jack Schuler, a beneficial owner of more than 10 %
of the Company's stock, is also a beneficial owner of more than 10 %
of the stock of Biodesix, Inc. Since the inception of the Biodesix Agreement, the Company has recorded $ 105,000
in costs under the Biodesix Agreement as research and development expense in our consolidated financial statement
of operations. As of December 31, 2024, the Company had $ 53,000
recorded as a current liability in its consolidated balance sheet.

 NOTE
12: SEGMENT REPORTING

 The
CODM for the Company is the Chief Executive Officer, who reviews financial information presented on a consolidated basis for purposes
of allocating resources and assessing financial performance.

 In
2024, the Company was managed as a single reporting unit associated with the discovery, development and commercialization of noninvasive
diagnostic tests. The accounting policies of the Company's single segment are the same as those described in the summary of significant
accounting policies in Note 1 to the consolidated financial statements. The CODM assesses the performance of the Company's single segment
and decides how to allocate resources based on consolidated net income. Under the current organizational structure, this measure is not
discreetly available or required individually for any of the Company's business activities and is only available at the consolidated
level. The monitoring of budgeted versus actual results are used in assessing performance of the Company's single segment, allocating
resources and in establishing management's compensation. The measure of segment assets is reported on the consolidated balance
sheet as total consolidated assets. Consolidated revenue does not include any inter-segment sales or transfers.

 The
following table summarizes financial statement line items regularly reviewed by the CODM (in thousands).

OF FINANCIAL STATEMENT

 2024

 2023

 Total Assets

 $
 5,491

 $
 6,262

 Total Revenue

 $
 9,182

 $
 9,154

 Net Loss

 $
)

 $
)

 F- 28

 NOTE
13: SUBSEQUENT EVENTS

 On
February 11, 2025, the Company received written notice from the Nasdaq Stock Market, LLC ("Nasdaq")
that based on the closing bid price per share immediately preceding entering into a binding agreement to issue the securities for the
Private Placement of $ 1.47 per share plus $ 0.125 attributable to the value of the warrants, the market value of the transaction for purposes
of Listing Rule 5625(c) was $ 1.595. Since the shares and warrants sold in the private placement were issued below the market value, and
the Company failed to obtain shareholder approval, the Company violated Listing Rule 5635(c). Accordingly, this matter served as an additional
basis for delisting the Company's securities from Nasdaq.

 Subsequently,
on February 11, 2025, the Company completed amendments to the warrants prohibiting exercise until shareholder approval has been
obtained. As a result, the Staff of Nasdaq has determined that the Company has regained compliance with Listing Rule 5635(c) and
subject to the disclosure requirements below, this matter is now closed.

 On March 5, 2025, the Company
entered into a securities purchase agreement with certain existing accredited shareholders ("the "Purchasers") for the
issuance and sale in a private placement (the "March 2025 Private Placement") of an aggregate principal amount of $ 1,365,000
in the form of Senior Secured Convertible Promissory Notes (the "Convertible Notes"), before deducted estimated costs of $ 50,000.

 The Convertible Notes,
which are convertible into units consisting of one share of common stock and 2.25 warrants (the "March 2025 Warrants"), will
be senior, secured obligations of the Company. Interest will accrue and be payable on a quarterly basis in kind at 3.4 %, the applicable
federal rate at the time of the transaction. The Convertible Notes will mature on March 6, 2030, unless earlier converted in accordance
with the terms of the Convertible Notes. In addition, the Company shall have the option to convert the Convertible Notes into units at
the Conversion Price if the sum of the net proceeds from the sale of the Convertible Notes and the net proceeds from the sale of
any shares of common stock and warrants by the Company subsequent to the March 2025 Private Placement equals or exceeds $ 4
 million.

 The March 2025 Warrants are exercisable
for five years at $ 0.25 per share for the first 24 months after issuance, and $ 0.50 per share thereafter.

 The holders of the Notes are entitled
to three representatives on the Company's board of directors.

 In addition, the
Company granted the Purchasers of the Convertible Notes certain customary registration rights with respect to the shares of common stock
and shares of common stock underlying the March 2025 Warrants. On March 6, 2025, the Company received wr itten
notice from Nasdaq that it would grant the Company's request for continued listing on the Nasdaq Capital Market subject to certain
conditions. Although the Company has been granted the conditional exception to remain listed on the Nasdaq Capital Market, no assurance
can be provided that it will be successfully meet the conditions of the exception and that its common stock will continue to be listed
on The Nasdaq Capital Market.

 Subsequent
to December 31, 2024 and through March 25, 2025, the Company sold 12,277,441 shares under the 2024 At the Market Offering Agreement
for gross proceeds of approximately $ 3,483,000 before deducting expenses of approximately $ 146,000. As of March 25, 2025, total
gross proceeds to the Company over the life of the At the Market Offering Agreement is $ 4,388,000 and the value of the remaining
availability was approximately $ 62,000 that could be sold to H.C. Wainwright under the 2024 At the
Market Offering Agreement, subject to the terms of the 2024 At the Market Offering Agreement.

 F- 29

 SIGNATURES

to the requirements of Section 13 or 15(d) of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed
on its behalf by the undersigned, thereunto duly authorized.

 Date:
 March 27, 2025

 /s/
 Michael Buhle

 Michael
 Buhle

 President
 and Chief Executive Officer (Principal Executive Officer)

 Date:
 March 27, 2025

 /s/
 James Crawford

 James
 Crawford

 Vice
 President of Finance (Principal Financial Officer and Principal Accounting Officer)

 POWER
OF ATTORNEY

 Each
of the undersigned officers and directors of Aspira Women's Health Inc., hereby constitutes and appoints Michael Buhle and James Crawford, and each or any of them, as their true and lawful attorney-in-fact and agent, for them and in their name, place and stead, in
any and all capacities, to sign their name to any and all amendments to this Report on Form 10-K, and other related documents, and to
cause the same to be filed with the Securities and Exchange Commission, granting unto said attorneys, full power and authority to do
and perform any act and thing necessary and proper to be done in the premises, as fully to all intents and purposes as the undersigned
could do if personally present, and the undersigned for himself hereby ratifies and confirms all that said attorney shall lawfully do
or cause to be done by virtue hereof.

to the requirements of the Securities Exchange Act of 1934, this report has been signed below by the following persons on behalf of the
registrant and in the capacities and on the dates indicated.

 Name

 Title

 Date

 /s/
 Michael Buhle

 President
 and Chief Executive Officer

 Michael
 Buhle

 (Principal
 Executive Officer) and Director

 March

 /s/
 James Crawford

 Vice
 President of Finance

 James
 Crawford

 (Principal
 Financial Officer and Principal Accounting Officer)

 March

 /s/
 Jannie Herchuk

 Chair
 of the Board of Directors

 March

 Jannie
 Herchuk

 /s/
 Ellen Beausang

 March

 Ellen
 Beausang

 /s/
 Stefanie Cavanaugh

 March

 Stefanie
 Cavanaugh

 /s/
 Celeste Fralick

 March

 Celeste
 Fralick

 /s/
 Ellen O'Connor-Vos

 March

 Ellen
 O'Connor-Vos

 /s/
 Winfred Parnell

 March

 Winfred
 Parnell

 /s/
 John Ragard

 March

 John
 Ragard